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Protective Effect of Solanum nigrum Leaves Extract on Immobilization Stress Induced Changes in Rat's Brain.

Zaidi SK, Hoda MN, Tabrez S, Ansari SA, Jafri MA, Shahnawaz Khan M, Hasan S, Alqahtani MH, Mohammed Abuzenadah A, Banu N - Evid Based Complement Alternat Med (2014)

Bottom Line: Treatment of S. nigrum extract and its active constituents to both pre- and poststressed rats resulted in significant modulation in the above mentioned parameters towards their control values with a relative dominance by the latter.Brain is vulnerable to stress induced prooxidant insult due to high levels of fat content.Thus, as a safe herbal medication the S. nigrum leaves extract or its isolated constituents can be used as nutritional supplement for scavenging free radicals generated in the brain due to physical or psychological stress or any neuronal diseases per se.

View Article: PubMed Central - PubMed

Affiliation: Center of Excellence in Genomic Medicine Research, King Abdulaziz University, Jeddah, Saudi Arabia.

ABSTRACT
The prophylactic or curative antioxidant efficacy of crude extract and the active constituent of S. nigrum leaves were evaluated in modulating inherent antioxidant system altered due to immobilization stress in rat brain tissues, in terms of measurement of glutathione (GSH), lipid peroxidation (thiobarbituric acid reactive substances, TBARS), and free radical scavenging enzymes activities. Rats were treated with single dose of crude extract of S. nigrum prior to and after 6 h of immobilization stress exposure. Exposure to immobilization stress resulted in a decrease in the brain levels of glutathione, SOD, GST, and catalase, with an increase in thiobarbituric acid reactive substances (TBARS) levels. Treatment of S. nigrum extract and its active constituents to both pre- and poststressed rats resulted in significant modulation in the above mentioned parameters towards their control values with a relative dominance by the latter. Brain is vulnerable to stress induced prooxidant insult due to high levels of fat content. Thus, as a safe herbal medication the S. nigrum leaves extract or its isolated constituents can be used as nutritional supplement for scavenging free radicals generated in the brain due to physical or psychological stress or any neuronal diseases per se.

No MeSH data available.


Related in: MedlinePlus

Extraction of active constituents from S. nigrum leaves extract.
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Related In: Results  -  Collection


getmorefigures.php?uid=PMC3934381&req=5

fig1: Extraction of active constituents from S. nigrum leaves extract.

Mentions: To elucidate the effect of S. nigrum extract on immobilization stress induced prooxidant changes [17], seventy rats were selected and divided into 14 groups of 5 rats each (5 groups for crude extract and 9 groups for active constituents). A flow diagram depicting extraction methodology of active constituents of S. nigrum has been provided (Figure 1). Dose and time dependent pilot study was performed to decide the dose and time of S. nigrum treatment (results not shown). It was observed that a single dose of 100 mg/kg body weight (bw) of extract prior to and after 1 h had the best preventive/curative effect on oxidative stress changes in the rat brain. The first group of animals received normal saline orally and served as controls. The second group of animals were given immobilization stress, the third group received a single dose of S. nigrum aqueous extract (100 mg/kg of bw) orally, and the fourth and fifth groups received extracts of the same dose 1 h prior to (prestress treatment) and 1 h after (poststress treatment) the 6 h session of stress. The effect of active constituent of S. nigrum on stress was studied separately and the groupings of those animals are provided in Table 1.


Protective Effect of Solanum nigrum Leaves Extract on Immobilization Stress Induced Changes in Rat's Brain.

Zaidi SK, Hoda MN, Tabrez S, Ansari SA, Jafri MA, Shahnawaz Khan M, Hasan S, Alqahtani MH, Mohammed Abuzenadah A, Banu N - Evid Based Complement Alternat Med (2014)

Extraction of active constituents from S. nigrum leaves extract.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3934381&req=5

fig1: Extraction of active constituents from S. nigrum leaves extract.
Mentions: To elucidate the effect of S. nigrum extract on immobilization stress induced prooxidant changes [17], seventy rats were selected and divided into 14 groups of 5 rats each (5 groups for crude extract and 9 groups for active constituents). A flow diagram depicting extraction methodology of active constituents of S. nigrum has been provided (Figure 1). Dose and time dependent pilot study was performed to decide the dose and time of S. nigrum treatment (results not shown). It was observed that a single dose of 100 mg/kg body weight (bw) of extract prior to and after 1 h had the best preventive/curative effect on oxidative stress changes in the rat brain. The first group of animals received normal saline orally and served as controls. The second group of animals were given immobilization stress, the third group received a single dose of S. nigrum aqueous extract (100 mg/kg of bw) orally, and the fourth and fifth groups received extracts of the same dose 1 h prior to (prestress treatment) and 1 h after (poststress treatment) the 6 h session of stress. The effect of active constituent of S. nigrum on stress was studied separately and the groupings of those animals are provided in Table 1.

Bottom Line: Treatment of S. nigrum extract and its active constituents to both pre- and poststressed rats resulted in significant modulation in the above mentioned parameters towards their control values with a relative dominance by the latter.Brain is vulnerable to stress induced prooxidant insult due to high levels of fat content.Thus, as a safe herbal medication the S. nigrum leaves extract or its isolated constituents can be used as nutritional supplement for scavenging free radicals generated in the brain due to physical or psychological stress or any neuronal diseases per se.

View Article: PubMed Central - PubMed

Affiliation: Center of Excellence in Genomic Medicine Research, King Abdulaziz University, Jeddah, Saudi Arabia.

ABSTRACT
The prophylactic or curative antioxidant efficacy of crude extract and the active constituent of S. nigrum leaves were evaluated in modulating inherent antioxidant system altered due to immobilization stress in rat brain tissues, in terms of measurement of glutathione (GSH), lipid peroxidation (thiobarbituric acid reactive substances, TBARS), and free radical scavenging enzymes activities. Rats were treated with single dose of crude extract of S. nigrum prior to and after 6 h of immobilization stress exposure. Exposure to immobilization stress resulted in a decrease in the brain levels of glutathione, SOD, GST, and catalase, with an increase in thiobarbituric acid reactive substances (TBARS) levels. Treatment of S. nigrum extract and its active constituents to both pre- and poststressed rats resulted in significant modulation in the above mentioned parameters towards their control values with a relative dominance by the latter. Brain is vulnerable to stress induced prooxidant insult due to high levels of fat content. Thus, as a safe herbal medication the S. nigrum leaves extract or its isolated constituents can be used as nutritional supplement for scavenging free radicals generated in the brain due to physical or psychological stress or any neuronal diseases per se.

No MeSH data available.


Related in: MedlinePlus