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Antimicrobial capacity of Aloe vera and propolis dentifrice against Streptococcus mutans strains in toothbrushes: an in vitro study.

Bertolini PF, Biondi Filho O, Pomilio A, Pinheiro SL, Carvalho MS - J Appl Oral Sci (2012)

Bottom Line: Scores were used to count the number of colony forming units (cfu).The results were submitted to the Mann-Whitney statistical test at 5% significance level.There was statistically significant difference (p<0.05) for the reduction of bristle contamination comparing groups II, III, IV and V to group I.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontics, Paulista University, Sorocaba, Brazil. bertolinipfr@hotmail.com

ABSTRACT

Objectives: This study evaluated in vitro the efficiency of Aloe vera and propolis dentifrice on reducing the contamination of toothbrush bristles by a standard strain of Streptococcus mutans (ATCC 25175; SM), after toothbrushing.

Material and methods: Fifteen sterile toothbrushes were randomly divided into 5 toothbrushing groups: I (negative control): without dentifrice; II: with fluoridated dentifrice; III: with triclosan and gantrez dentifrice; IV (positive control): without dentifrice and irrigation with 10 mL of 0.12% chlorhexidine gluconate; V: with Aloe vera and propolis dentifrice. In each group, 1 sterile bovine tooth was brushed for 1 min, where the toothbrush bristles were contaminated with 25 µL of SM. After toothbrushing, the bristles were stored in individual test tubes with 3 mL of BHI under anaerobiosis of 37°C for 48 h. Then, they were seeded with sterile swab in triplicate in the Mitis salivarius - Bacitracin culture medium. The samples were kept under anaerobiosis of 37°C for 48 h. Scores were used to count the number of colony forming units (cfu). The results were submitted to the Mann-Whitney statistical test at 5% significance level.

Results: There was statistically significant difference (p<0.05) for the reduction of bristle contamination comparing groups II, III, IV and V to group I.

Conclusions: It may be stated that after toothbrushing, the Aloe vera and propolis dentifrice reduced the contamination of toothbrush bristles by SM, without differentiation from the other chemical agents used.

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Characteristic of bacterial growth for group I, II, III, IV and V in the culturemedium
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f05: Characteristic of bacterial growth for group I, II, III, IV and V in the culturemedium

Mentions: The scores assigned to the cfu's counted for each group are shown in Table 1, where a statistically significantdifference was found (p<0.05) in the reduction of bristle contamination in groups II,III, IV and V compared to group I. However, there was no statistically significantdifference among the groups that used the chemical agents to decontaminate thetoothbrush bristles (Figure 4 and Figure 5).


Antimicrobial capacity of Aloe vera and propolis dentifrice against Streptococcus mutans strains in toothbrushes: an in vitro study.

Bertolini PF, Biondi Filho O, Pomilio A, Pinheiro SL, Carvalho MS - J Appl Oral Sci (2012)

Characteristic of bacterial growth for group I, II, III, IV and V in the culturemedium
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3928769&req=5

f05: Characteristic of bacterial growth for group I, II, III, IV and V in the culturemedium
Mentions: The scores assigned to the cfu's counted for each group are shown in Table 1, where a statistically significantdifference was found (p<0.05) in the reduction of bristle contamination in groups II,III, IV and V compared to group I. However, there was no statistically significantdifference among the groups that used the chemical agents to decontaminate thetoothbrush bristles (Figure 4 and Figure 5).

Bottom Line: Scores were used to count the number of colony forming units (cfu).The results were submitted to the Mann-Whitney statistical test at 5% significance level.There was statistically significant difference (p<0.05) for the reduction of bristle contamination comparing groups II, III, IV and V to group I.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontics, Paulista University, Sorocaba, Brazil. bertolinipfr@hotmail.com

ABSTRACT

Objectives: This study evaluated in vitro the efficiency of Aloe vera and propolis dentifrice on reducing the contamination of toothbrush bristles by a standard strain of Streptococcus mutans (ATCC 25175; SM), after toothbrushing.

Material and methods: Fifteen sterile toothbrushes were randomly divided into 5 toothbrushing groups: I (negative control): without dentifrice; II: with fluoridated dentifrice; III: with triclosan and gantrez dentifrice; IV (positive control): without dentifrice and irrigation with 10 mL of 0.12% chlorhexidine gluconate; V: with Aloe vera and propolis dentifrice. In each group, 1 sterile bovine tooth was brushed for 1 min, where the toothbrush bristles were contaminated with 25 µL of SM. After toothbrushing, the bristles were stored in individual test tubes with 3 mL of BHI under anaerobiosis of 37°C for 48 h. Then, they were seeded with sterile swab in triplicate in the Mitis salivarius - Bacitracin culture medium. The samples were kept under anaerobiosis of 37°C for 48 h. Scores were used to count the number of colony forming units (cfu). The results were submitted to the Mann-Whitney statistical test at 5% significance level.

Results: There was statistically significant difference (p<0.05) for the reduction of bristle contamination comparing groups II, III, IV and V to group I.

Conclusions: It may be stated that after toothbrushing, the Aloe vera and propolis dentifrice reduced the contamination of toothbrush bristles by SM, without differentiation from the other chemical agents used.

Show MeSH
Related in: MedlinePlus