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Antibacterial Activity of Rhizome of Curcuma aromatica and Partial Purification of Active Compounds.

Revathi S, Malathy NS - Indian J Pharm Sci (2013)

Bottom Line: The hexane extract was found to be active against all Gram-positive strains tested, but inactive against Gram-negative strains.The minimum inhibitory concentration and minimum bactericidal concentration were determined and found to be 539 μg/ml.The zone showing good antibacterial activity was analysed further by gas chromatography mass spectrometry, UV/Vis spectrophotometry and Fourier transform infrared spectroscopy, which indicated the probable presence of germacrone.

View Article: PubMed Central - PubMed

Affiliation: Departments of Plant Biology and Plant Biotechnology, PSGR. Krishnammal College for Women, Coimbatore-641004, India.

ABSTRACT
The hexane extract of Curcuma aromatica, a plant belonging to the family Zingiberaceae was tested on 10 bacterial strains (clinical isolates and standard strains). Agar diffusion method was adopted for determining the antibacterial activity of the extract. The hexane extract was found to be active against all Gram-positive strains tested, but inactive against Gram-negative strains. The minimum inhibitory concentration and minimum bactericidal concentration were determined and found to be 539 μg/ml. The phytochemical analysis of hexane extract by gas chromatography mass spectrometry revealed the presence of 13 compounds. The crude hexane extract was partially purified by thin layer chromatography. The zone showing good antibacterial activity was analysed further by gas chromatography mass spectrometry, UV/Vis spectrophotometry and Fourier transform infrared spectroscopy, which indicated the probable presence of germacrone.

No MeSH data available.


FT-IR spectrum of the zone 5 of the hexane extract. FT-IR of the zone 5 from TLC of hexane extract of the rhizomes of C. aromaticus.
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Figure 2: FT-IR spectrum of the zone 5 of the hexane extract. FT-IR of the zone 5 from TLC of hexane extract of the rhizomes of C. aromaticus.

Mentions: Partial purification of the extract by TLC, showed that the Zone 5 was inhibitory even at a low concentration of 5 μl/disc as shown in Table 4. The phytochemical analysis for this zone by GC-MS (Table 5), indicated the presence of germacrone. The UV/Vis absorption spectrum (fig. 1) and FT-IR spectrum (fig. 2) of this zone also indicated the presence of germacrone. Germacrone is reported for its root weevil repellent[19] and antimicrobial properties[20], this supports the current findings.


Antibacterial Activity of Rhizome of Curcuma aromatica and Partial Purification of Active Compounds.

Revathi S, Malathy NS - Indian J Pharm Sci (2013)

FT-IR spectrum of the zone 5 of the hexane extract. FT-IR of the zone 5 from TLC of hexane extract of the rhizomes of C. aromaticus.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3928740&req=5

Figure 2: FT-IR spectrum of the zone 5 of the hexane extract. FT-IR of the zone 5 from TLC of hexane extract of the rhizomes of C. aromaticus.
Mentions: Partial purification of the extract by TLC, showed that the Zone 5 was inhibitory even at a low concentration of 5 μl/disc as shown in Table 4. The phytochemical analysis for this zone by GC-MS (Table 5), indicated the presence of germacrone. The UV/Vis absorption spectrum (fig. 1) and FT-IR spectrum (fig. 2) of this zone also indicated the presence of germacrone. Germacrone is reported for its root weevil repellent[19] and antimicrobial properties[20], this supports the current findings.

Bottom Line: The hexane extract was found to be active against all Gram-positive strains tested, but inactive against Gram-negative strains.The minimum inhibitory concentration and minimum bactericidal concentration were determined and found to be 539 μg/ml.The zone showing good antibacterial activity was analysed further by gas chromatography mass spectrometry, UV/Vis spectrophotometry and Fourier transform infrared spectroscopy, which indicated the probable presence of germacrone.

View Article: PubMed Central - PubMed

Affiliation: Departments of Plant Biology and Plant Biotechnology, PSGR. Krishnammal College for Women, Coimbatore-641004, India.

ABSTRACT
The hexane extract of Curcuma aromatica, a plant belonging to the family Zingiberaceae was tested on 10 bacterial strains (clinical isolates and standard strains). Agar diffusion method was adopted for determining the antibacterial activity of the extract. The hexane extract was found to be active against all Gram-positive strains tested, but inactive against Gram-negative strains. The minimum inhibitory concentration and minimum bactericidal concentration were determined and found to be 539 μg/ml. The phytochemical analysis of hexane extract by gas chromatography mass spectrometry revealed the presence of 13 compounds. The crude hexane extract was partially purified by thin layer chromatography. The zone showing good antibacterial activity was analysed further by gas chromatography mass spectrometry, UV/Vis spectrophotometry and Fourier transform infrared spectroscopy, which indicated the probable presence of germacrone.

No MeSH data available.