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Fluorimetric quantification of brimonidine tartrate in eye drops.

Sunitha G, Bhagirath R, Alapati VR, Ramakrishna K, Subrahmanyam CV, Anumolu PD - Indian J Pharm Sci (2013)

Bottom Line: Linearity was obeyed in the range of 0.2-3.0 ΅g/ml in dimethyl formamide as solvent at an emission wavelength (λem) of 530 nm after excitation wavelength (λex) of 389 nm with good correlation coefficient of 0.998.The percentage relative standard deviation values were found to be less than 2 for accuracy and precision studies.The results obtained were in good agreement with the labelled amounts of the marketed formulations.

View Article: PubMed Central - PubMed

Affiliation: Gokaraju Rangaraju College of Pharmacy, Osmania University, Hyderabad-500 090, India.

ABSTRACT
A simple and sensitive spectrofluorimetric method has been developed for the estimation of brimonidine tartrate in pure and eye drops. Linearity was obeyed in the range of 0.2-3.0 ΅g/ml in dimethyl formamide as solvent at an emission wavelength (λem) of 530 nm after excitation wavelength (λex) of 389 nm with good correlation coefficient of 0.998. The limit of detection and limit of quantification for this method were 22.0 and 72.0 ng/ml, respectively. The developed method was statistically validated as per International Conference on Harmonisation guidelines. The percentage relative standard deviation values were found to be less than 2 for accuracy and precision studies. The results obtained were in good agreement with the labelled amounts of the marketed formulations. The proposed method was effectively applied to routine quality control analysis of brimonidine tartrate in their eye drops.

No MeSH data available.


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Mentions: BRT molecule contains polycyclic aromatic systems like imidazole ring and quinoxaline ring, in which more π electrons are available to exhibit florescence. For spectrofluorimetric method purpose, various solvents were investigated such as acetonitrile, methanol, dimethyl sulphoxide (DMSO), ethanol, DMF and different buffer systems. The fluorescence intensity of BRT was found only in DMF solvent. Hence, we selected DMF as solvent for quantification of BRT in eye drops at emission wavelength 530 nm after excitation wave length 389 nm as shown in fig. 1.


Fluorimetric quantification of brimonidine tartrate in eye drops.

Sunitha G, Bhagirath R, Alapati VR, Ramakrishna K, Subrahmanyam CV, Anumolu PD - Indian J Pharm Sci (2013)

© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3928739&req=5

Mentions: BRT molecule contains polycyclic aromatic systems like imidazole ring and quinoxaline ring, in which more π electrons are available to exhibit florescence. For spectrofluorimetric method purpose, various solvents were investigated such as acetonitrile, methanol, dimethyl sulphoxide (DMSO), ethanol, DMF and different buffer systems. The fluorescence intensity of BRT was found only in DMF solvent. Hence, we selected DMF as solvent for quantification of BRT in eye drops at emission wavelength 530 nm after excitation wave length 389 nm as shown in fig. 1.

Bottom Line: Linearity was obeyed in the range of 0.2-3.0 ΅g/ml in dimethyl formamide as solvent at an emission wavelength (λem) of 530 nm after excitation wavelength (λex) of 389 nm with good correlation coefficient of 0.998.The percentage relative standard deviation values were found to be less than 2 for accuracy and precision studies.The results obtained were in good agreement with the labelled amounts of the marketed formulations.

View Article: PubMed Central - PubMed

Affiliation: Gokaraju Rangaraju College of Pharmacy, Osmania University, Hyderabad-500 090, India.

ABSTRACT
A simple and sensitive spectrofluorimetric method has been developed for the estimation of brimonidine tartrate in pure and eye drops. Linearity was obeyed in the range of 0.2-3.0 ΅g/ml in dimethyl formamide as solvent at an emission wavelength (λem) of 530 nm after excitation wavelength (λex) of 389 nm with good correlation coefficient of 0.998. The limit of detection and limit of quantification for this method were 22.0 and 72.0 ng/ml, respectively. The developed method was statistically validated as per International Conference on Harmonisation guidelines. The percentage relative standard deviation values were found to be less than 2 for accuracy and precision studies. The results obtained were in good agreement with the labelled amounts of the marketed formulations. The proposed method was effectively applied to routine quality control analysis of brimonidine tartrate in their eye drops.

No MeSH data available.