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Mosla punctulata Inhibits Mast Cell-mediated Allergic Reactions Through the Inhibition of Histamine Release and Inflammatory Cytokine Production.

Je IG, Shin TY, Kim SH - Indian J Pharm Sci (2013)

Bottom Line: Allergic inflammatory diseases such as food allergy, asthma, sinusitis and atopic dermatitis are increasing worldwide.The results indicate that aqueous extract of Mosla punctulata inhibited mast cell-mediated allergic inflammatory reaction by suppressing histamine release and expression of tumour necrosis factor alpha, and involvement of calcium and nuclear factor kappa B in these effects.Hence it can be concluded that, the aqueous extract of Mosla punctulata might be a possible therapeutic candidate for allergic inflammatory disorders.

View Article: PubMed Central - PubMed

Affiliation: CMRI, Department of Pharmacology, School of Medicine, Kyungpook National University, Daegu-700 422, Republic of Korea.

ABSTRACT
Allergic inflammatory diseases such as food allergy, asthma, sinusitis and atopic dermatitis are increasing worldwide. This study examined the effects of aqueous extract of Mosla punctulata on mast cell-mediated allergic inflammation and studied the possible mechanism of action. Aqueous extract of Mosla punctulata inhibited compound 48/80-induced systemic and immunoglobulin E-mediated local anaphylaxis and it also reduced intracellular calcium level and down-streamed histamine release from mast cells. In addition, aqueous extract of Mosla punctulata decreased gene expression and secretion of tumour necrosis factor alpha, an important proinflammatory cytokine, in mast cells. The inhibitory effect on tumour necrosis factor alpha expression was nuclear factor kappa B dependent. The results indicate that aqueous extract of Mosla punctulata inhibited mast cell-mediated allergic inflammatory reaction by suppressing histamine release and expression of tumour necrosis factor alpha, and involvement of calcium and nuclear factor kappa B in these effects. Hence it can be concluded that, the aqueous extract of Mosla punctulata might be a possible therapeutic candidate for allergic inflammatory disorders.

No MeSH data available.


Related in: MedlinePlus

Effects of AEMP on the activation of NF-κB.HMC-1 cells were pre-treated with AEMP for 30 min prior to PMA (20 nM) and A23187 (1 μM) stimulation for 2 h. (a) Nuclear translocation of NF-κB was assayed by Western blot (N-NF-κB, nuclear NF-κB). (b) Cells were transiently transfected with the NF-κB-luciferase reporter construct or empty vector. Then, the cells were incubated with PMA and A23187 with or without AEMP. NF-κB-dependent transcriptional activity was determined by luciferase activity assay. Each bar represents the mean±SEM of three independent experiments. *Significant difference from PMACI value at P<0.05.
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Figure 4: Effects of AEMP on the activation of NF-κB.HMC-1 cells were pre-treated with AEMP for 30 min prior to PMA (20 nM) and A23187 (1 μM) stimulation for 2 h. (a) Nuclear translocation of NF-κB was assayed by Western blot (N-NF-κB, nuclear NF-κB). (b) Cells were transiently transfected with the NF-κB-luciferase reporter construct or empty vector. Then, the cells were incubated with PMA and A23187 with or without AEMP. NF-κB-dependent transcriptional activity was determined by luciferase activity assay. Each bar represents the mean±SEM of three independent experiments. *Significant difference from PMACI value at P<0.05.

Mentions: To investigate the intracellular mechanism responsible for the inhibitory effect of AEMP on the expression of TNF-α, we examined the effect of AEMP on the activation of transcription factors, NF-κB. NF-κB is an important transcriptional regulator of TNF-α and plays a crucial role in immune and inflammatory responses[16]. Stimulation of HMC-1 with PMACI induced the nuclear translocation of p65 NF-κB after 2 h of incubation. AEMP inhibited the PMACI-induced nuclear translocation of NF-κB (fig. 4a). To confirm the inhibitory effect of AEMP on the NF-κB activation, we examined the effect of AEMP on NF-κB-dependent gene reporter assay. HMC-1 cells were transiently transfected with a NF-κB-luciferase reporter construct or an empty vector. Exposure of cells to PMACI increased the luciferase activity in the cells transfected with NF-κB-luciferase reporter construct (fig. 4b). AEMP significantly reduced PMACI-induced luciferase activity.


Mosla punctulata Inhibits Mast Cell-mediated Allergic Reactions Through the Inhibition of Histamine Release and Inflammatory Cytokine Production.

Je IG, Shin TY, Kim SH - Indian J Pharm Sci (2013)

Effects of AEMP on the activation of NF-κB.HMC-1 cells were pre-treated with AEMP for 30 min prior to PMA (20 nM) and A23187 (1 μM) stimulation for 2 h. (a) Nuclear translocation of NF-κB was assayed by Western blot (N-NF-κB, nuclear NF-κB). (b) Cells were transiently transfected with the NF-κB-luciferase reporter construct or empty vector. Then, the cells were incubated with PMA and A23187 with or without AEMP. NF-κB-dependent transcriptional activity was determined by luciferase activity assay. Each bar represents the mean±SEM of three independent experiments. *Significant difference from PMACI value at P<0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3928730&req=5

Figure 4: Effects of AEMP on the activation of NF-κB.HMC-1 cells were pre-treated with AEMP for 30 min prior to PMA (20 nM) and A23187 (1 μM) stimulation for 2 h. (a) Nuclear translocation of NF-κB was assayed by Western blot (N-NF-κB, nuclear NF-κB). (b) Cells were transiently transfected with the NF-κB-luciferase reporter construct or empty vector. Then, the cells were incubated with PMA and A23187 with or without AEMP. NF-κB-dependent transcriptional activity was determined by luciferase activity assay. Each bar represents the mean±SEM of three independent experiments. *Significant difference from PMACI value at P<0.05.
Mentions: To investigate the intracellular mechanism responsible for the inhibitory effect of AEMP on the expression of TNF-α, we examined the effect of AEMP on the activation of transcription factors, NF-κB. NF-κB is an important transcriptional regulator of TNF-α and plays a crucial role in immune and inflammatory responses[16]. Stimulation of HMC-1 with PMACI induced the nuclear translocation of p65 NF-κB after 2 h of incubation. AEMP inhibited the PMACI-induced nuclear translocation of NF-κB (fig. 4a). To confirm the inhibitory effect of AEMP on the NF-κB activation, we examined the effect of AEMP on NF-κB-dependent gene reporter assay. HMC-1 cells were transiently transfected with a NF-κB-luciferase reporter construct or an empty vector. Exposure of cells to PMACI increased the luciferase activity in the cells transfected with NF-κB-luciferase reporter construct (fig. 4b). AEMP significantly reduced PMACI-induced luciferase activity.

Bottom Line: Allergic inflammatory diseases such as food allergy, asthma, sinusitis and atopic dermatitis are increasing worldwide.The results indicate that aqueous extract of Mosla punctulata inhibited mast cell-mediated allergic inflammatory reaction by suppressing histamine release and expression of tumour necrosis factor alpha, and involvement of calcium and nuclear factor kappa B in these effects.Hence it can be concluded that, the aqueous extract of Mosla punctulata might be a possible therapeutic candidate for allergic inflammatory disorders.

View Article: PubMed Central - PubMed

Affiliation: CMRI, Department of Pharmacology, School of Medicine, Kyungpook National University, Daegu-700 422, Republic of Korea.

ABSTRACT
Allergic inflammatory diseases such as food allergy, asthma, sinusitis and atopic dermatitis are increasing worldwide. This study examined the effects of aqueous extract of Mosla punctulata on mast cell-mediated allergic inflammation and studied the possible mechanism of action. Aqueous extract of Mosla punctulata inhibited compound 48/80-induced systemic and immunoglobulin E-mediated local anaphylaxis and it also reduced intracellular calcium level and down-streamed histamine release from mast cells. In addition, aqueous extract of Mosla punctulata decreased gene expression and secretion of tumour necrosis factor alpha, an important proinflammatory cytokine, in mast cells. The inhibitory effect on tumour necrosis factor alpha expression was nuclear factor kappa B dependent. The results indicate that aqueous extract of Mosla punctulata inhibited mast cell-mediated allergic inflammatory reaction by suppressing histamine release and expression of tumour necrosis factor alpha, and involvement of calcium and nuclear factor kappa B in these effects. Hence it can be concluded that, the aqueous extract of Mosla punctulata might be a possible therapeutic candidate for allergic inflammatory disorders.

No MeSH data available.


Related in: MedlinePlus