Limits...
Mosla punctulata Inhibits Mast Cell-mediated Allergic Reactions Through the Inhibition of Histamine Release and Inflammatory Cytokine Production.

Je IG, Shin TY, Kim SH - Indian J Pharm Sci (2013)

Bottom Line: Allergic inflammatory diseases such as food allergy, asthma, sinusitis and atopic dermatitis are increasing worldwide.The results indicate that aqueous extract of Mosla punctulata inhibited mast cell-mediated allergic inflammatory reaction by suppressing histamine release and expression of tumour necrosis factor alpha, and involvement of calcium and nuclear factor kappa B in these effects.Hence it can be concluded that, the aqueous extract of Mosla punctulata might be a possible therapeutic candidate for allergic inflammatory disorders.

View Article: PubMed Central - PubMed

Affiliation: CMRI, Department of Pharmacology, School of Medicine, Kyungpook National University, Daegu-700 422, Republic of Korea.

ABSTRACT
Allergic inflammatory diseases such as food allergy, asthma, sinusitis and atopic dermatitis are increasing worldwide. This study examined the effects of aqueous extract of Mosla punctulata on mast cell-mediated allergic inflammation and studied the possible mechanism of action. Aqueous extract of Mosla punctulata inhibited compound 48/80-induced systemic and immunoglobulin E-mediated local anaphylaxis and it also reduced intracellular calcium level and down-streamed histamine release from mast cells. In addition, aqueous extract of Mosla punctulata decreased gene expression and secretion of tumour necrosis factor alpha, an important proinflammatory cytokine, in mast cells. The inhibitory effect on tumour necrosis factor alpha expression was nuclear factor kappa B dependent. The results indicate that aqueous extract of Mosla punctulata inhibited mast cell-mediated allergic inflammatory reaction by suppressing histamine release and expression of tumour necrosis factor alpha, and involvement of calcium and nuclear factor kappa B in these effects. Hence it can be concluded that, the aqueous extract of Mosla punctulata might be a possible therapeutic candidate for allergic inflammatory disorders.

No MeSH data available.


Related in: MedlinePlus

Effects of AEMP on serum histamine release and PCA reaction.(a) Groups of mice (n=10) were intraperitoneally pretreated with 200 μl of saline or AEMP at various doses 1 h before the intraperitoneal injection of compound 48/80. The blood was obtained from the heart of each mouse and histamine content was measured. (b) AEMP was intraperitoneally administered 1 h prior to the challenge with antigen (n=5). Each amount of dye was extracted as described in ‘Materials and Methods’ section, and measured by spectrophotometry. Each bar represents the mean±SEM of three independent experiments. *Significant difference at P<0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3928730&req=5

Figure 1: Effects of AEMP on serum histamine release and PCA reaction.(a) Groups of mice (n=10) were intraperitoneally pretreated with 200 μl of saline or AEMP at various doses 1 h before the intraperitoneal injection of compound 48/80. The blood was obtained from the heart of each mouse and histamine content was measured. (b) AEMP was intraperitoneally administered 1 h prior to the challenge with antigen (n=5). Each amount of dye was extracted as described in ‘Materials and Methods’ section, and measured by spectrophotometry. Each bar represents the mean±SEM of three independent experiments. *Significant difference at P<0.05.

Mentions: To determine the effect of AEMP in allergic reaction, an in vivo model of a systemic anaphylaxis was used. After the intraperitoneal injection of compound 48/80, the mice were monitored for 1 h, after which the mortality rate was determined. Injection of compound 48/80 (8 mg/kg) into mice induced fatal shock in 100% of animals. When AEMP was intraperitoneally pretreated at doses ranging from 1 to 1000 mg/kg for 1 h, the mortality was dose dependently reduced. AEMP at dose of 1000 mg/kg completely inhibited compound 48/80-induced fatal shock (Table 1). In addition, the mortality of mice administered with AEMP (1000 mg/kg) 5, 10, 15 and 20 min after compound 48/80 injection increased time dependently (Table 2). The effect of AEMP on the compound 48/80-induced serum histamine release was investigated as well. AEMP was intraperitoneally administered 1 h prior to the injection of compound 48/80. Injection of compound 48/80 markedly caused an increase in serum histamine release, which was inhibited by AEMP treatment in a dose-dependent manner (fig. 1a). To confirm the antiallergic effects of AEMP, we used a PCA model induced by antiDNP-IgE and DNP-HSA. To compare the amount of dye with control, the left dorsal skin of these mice was injected with saline alone. AEMP (1-1000 mg/kg) was intraperitoneally administered 1 h prior to the challenge with antigen. AEMP dose-dependently inhibited PCA reaction (fig. 1b).


Mosla punctulata Inhibits Mast Cell-mediated Allergic Reactions Through the Inhibition of Histamine Release and Inflammatory Cytokine Production.

Je IG, Shin TY, Kim SH - Indian J Pharm Sci (2013)

Effects of AEMP on serum histamine release and PCA reaction.(a) Groups of mice (n=10) were intraperitoneally pretreated with 200 μl of saline or AEMP at various doses 1 h before the intraperitoneal injection of compound 48/80. The blood was obtained from the heart of each mouse and histamine content was measured. (b) AEMP was intraperitoneally administered 1 h prior to the challenge with antigen (n=5). Each amount of dye was extracted as described in ‘Materials and Methods’ section, and measured by spectrophotometry. Each bar represents the mean±SEM of three independent experiments. *Significant difference at P<0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3928730&req=5

Figure 1: Effects of AEMP on serum histamine release and PCA reaction.(a) Groups of mice (n=10) were intraperitoneally pretreated with 200 μl of saline or AEMP at various doses 1 h before the intraperitoneal injection of compound 48/80. The blood was obtained from the heart of each mouse and histamine content was measured. (b) AEMP was intraperitoneally administered 1 h prior to the challenge with antigen (n=5). Each amount of dye was extracted as described in ‘Materials and Methods’ section, and measured by spectrophotometry. Each bar represents the mean±SEM of three independent experiments. *Significant difference at P<0.05.
Mentions: To determine the effect of AEMP in allergic reaction, an in vivo model of a systemic anaphylaxis was used. After the intraperitoneal injection of compound 48/80, the mice were monitored for 1 h, after which the mortality rate was determined. Injection of compound 48/80 (8 mg/kg) into mice induced fatal shock in 100% of animals. When AEMP was intraperitoneally pretreated at doses ranging from 1 to 1000 mg/kg for 1 h, the mortality was dose dependently reduced. AEMP at dose of 1000 mg/kg completely inhibited compound 48/80-induced fatal shock (Table 1). In addition, the mortality of mice administered with AEMP (1000 mg/kg) 5, 10, 15 and 20 min after compound 48/80 injection increased time dependently (Table 2). The effect of AEMP on the compound 48/80-induced serum histamine release was investigated as well. AEMP was intraperitoneally administered 1 h prior to the injection of compound 48/80. Injection of compound 48/80 markedly caused an increase in serum histamine release, which was inhibited by AEMP treatment in a dose-dependent manner (fig. 1a). To confirm the antiallergic effects of AEMP, we used a PCA model induced by antiDNP-IgE and DNP-HSA. To compare the amount of dye with control, the left dorsal skin of these mice was injected with saline alone. AEMP (1-1000 mg/kg) was intraperitoneally administered 1 h prior to the challenge with antigen. AEMP dose-dependently inhibited PCA reaction (fig. 1b).

Bottom Line: Allergic inflammatory diseases such as food allergy, asthma, sinusitis and atopic dermatitis are increasing worldwide.The results indicate that aqueous extract of Mosla punctulata inhibited mast cell-mediated allergic inflammatory reaction by suppressing histamine release and expression of tumour necrosis factor alpha, and involvement of calcium and nuclear factor kappa B in these effects.Hence it can be concluded that, the aqueous extract of Mosla punctulata might be a possible therapeutic candidate for allergic inflammatory disorders.

View Article: PubMed Central - PubMed

Affiliation: CMRI, Department of Pharmacology, School of Medicine, Kyungpook National University, Daegu-700 422, Republic of Korea.

ABSTRACT
Allergic inflammatory diseases such as food allergy, asthma, sinusitis and atopic dermatitis are increasing worldwide. This study examined the effects of aqueous extract of Mosla punctulata on mast cell-mediated allergic inflammation and studied the possible mechanism of action. Aqueous extract of Mosla punctulata inhibited compound 48/80-induced systemic and immunoglobulin E-mediated local anaphylaxis and it also reduced intracellular calcium level and down-streamed histamine release from mast cells. In addition, aqueous extract of Mosla punctulata decreased gene expression and secretion of tumour necrosis factor alpha, an important proinflammatory cytokine, in mast cells. The inhibitory effect on tumour necrosis factor alpha expression was nuclear factor kappa B dependent. The results indicate that aqueous extract of Mosla punctulata inhibited mast cell-mediated allergic inflammatory reaction by suppressing histamine release and expression of tumour necrosis factor alpha, and involvement of calcium and nuclear factor kappa B in these effects. Hence it can be concluded that, the aqueous extract of Mosla punctulata might be a possible therapeutic candidate for allergic inflammatory disorders.

No MeSH data available.


Related in: MedlinePlus