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Pharmacognostic standardisation and antiproliferative activity of Aegle marmelos (L.) Correa leaves in various human cancer cell lines.

Bhatti R, Singh J, Saxena AK, Suri N, Ishar MP - Indian J Pharm Sci (2013)

Bottom Line: The phytochemical studies indicated alkaloids, anthraquinones, terpenoids in the alcohol, chloroform extracts and tannins, terpenoids, reducing sugars in the petroleum ether and hexane extracts.Out of this, fractions 2, 4 and 5 showed significant inhibition in leukaemia cell line with IC50 of 12.5, 86.2 and >100 μg/ml for fractions 2, 4 and 5, respectively.Among the different extracts investigated, ethanol extract exhibited significant antiproliferative activity and its fraction 2 containing furanocoumarin imperatorin showed antiproliferative activity against leukaemia cell line with IC50 of 12.5 μg/ml.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Sciences, Guru Nanak Dev University, Amritsar-143 005, India.

ABSTRACT
Therapeutic management of cancer is a great clinical challenge and alternative medicines are being extensively explored to have integrated approach to cure cancer. Aegle marmelos (L.) Correa (Rutaceae) is known for its hypoglycaemic, radioprotective, antidiarrhoeal and many other pharmacological activities. The present study is designed to carryout pharmacognostic standardisation and evaluation of antiproliferative activity of the leaf extracts Aegle marmelos (L.) Correa (Rutaceae) and the chromatographic fractions of the most active extract. Hexane, petroleum ether, chloroform and ethanol extracts of the shade dried leaves were prepared by soxhelation and antiproliferative activity was assessed using human cancer cell lines of lung (A-549), colon (CoLo-05), ovary (IGR-OV-1), prostrate (PC3), leukaemia (THP-1) and breast (MCF-7) cancer. Bioactivity-derived fractionation was carried out for most active extract by column chromatography. The phytochemical studies indicated alkaloids, anthraquinones, terpenoids in the alcohol, chloroform extracts and tannins, terpenoids, reducing sugars in the petroleum ether and hexane extracts. Ethanol extract showed maximum inhibition in colon and breast carcinoma cell lines at a dose of 100 μg/ml. Column chromatography of the ethanol extract yielded five fractions. Out of this, fractions 2, 4 and 5 showed significant inhibition in leukaemia cell line with IC50 of 12.5, 86.2 and >100 μg/ml for fractions 2, 4 and 5, respectively. High-performance thin layer chromatography of the fraction 2 revealed imperatorin as one of the major phytoconstituents. Among the different extracts investigated, ethanol extract exhibited significant antiproliferative activity and its fraction 2 containing furanocoumarin imperatorin showed antiproliferative activity against leukaemia cell line with IC50 of 12.5 μg/ml.

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Mentions: The effect of the various extracts on different cancer cell lines indicated that ethanol and chloroform extracts had significant anticancer activity against some cancer cell lines. The ethanol extract was found to be the most active and it showed 69% inhibition in both colon and breast carcinoma cell lines, which were comparable to the standard drug, that is adriamycin while inhibition in lung cancer cell lines was relatively less (42%). The chloroform extract showed 68% and 55% inhibition in breast and colon cancer cell lines, respectively. The petroleum ether extract and hexane extract showed negligible anti-cancer activity in all cancer cell lines (fig. 5). The various fractions of the ethanol extract were studied for anticancer activity and of the various fractions AME-1, AME-2, AME-3, AME-4 and AME-5 showed anticancer activity (fig. 6). The IC50 values were studied for fractions AME-2, AME-4 and AME-5 and were found to be 12.5, 86.2 and >100 μg/ml, respectively, in leukaemia cell line (THP-1).


Pharmacognostic standardisation and antiproliferative activity of Aegle marmelos (L.) Correa leaves in various human cancer cell lines.

Bhatti R, Singh J, Saxena AK, Suri N, Ishar MP - Indian J Pharm Sci (2013)

© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3928725&req=5

Mentions: The effect of the various extracts on different cancer cell lines indicated that ethanol and chloroform extracts had significant anticancer activity against some cancer cell lines. The ethanol extract was found to be the most active and it showed 69% inhibition in both colon and breast carcinoma cell lines, which were comparable to the standard drug, that is adriamycin while inhibition in lung cancer cell lines was relatively less (42%). The chloroform extract showed 68% and 55% inhibition in breast and colon cancer cell lines, respectively. The petroleum ether extract and hexane extract showed negligible anti-cancer activity in all cancer cell lines (fig. 5). The various fractions of the ethanol extract were studied for anticancer activity and of the various fractions AME-1, AME-2, AME-3, AME-4 and AME-5 showed anticancer activity (fig. 6). The IC50 values were studied for fractions AME-2, AME-4 and AME-5 and were found to be 12.5, 86.2 and >100 μg/ml, respectively, in leukaemia cell line (THP-1).

Bottom Line: The phytochemical studies indicated alkaloids, anthraquinones, terpenoids in the alcohol, chloroform extracts and tannins, terpenoids, reducing sugars in the petroleum ether and hexane extracts.Out of this, fractions 2, 4 and 5 showed significant inhibition in leukaemia cell line with IC50 of 12.5, 86.2 and >100 μg/ml for fractions 2, 4 and 5, respectively.Among the different extracts investigated, ethanol extract exhibited significant antiproliferative activity and its fraction 2 containing furanocoumarin imperatorin showed antiproliferative activity against leukaemia cell line with IC50 of 12.5 μg/ml.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Sciences, Guru Nanak Dev University, Amritsar-143 005, India.

ABSTRACT
Therapeutic management of cancer is a great clinical challenge and alternative medicines are being extensively explored to have integrated approach to cure cancer. Aegle marmelos (L.) Correa (Rutaceae) is known for its hypoglycaemic, radioprotective, antidiarrhoeal and many other pharmacological activities. The present study is designed to carryout pharmacognostic standardisation and evaluation of antiproliferative activity of the leaf extracts Aegle marmelos (L.) Correa (Rutaceae) and the chromatographic fractions of the most active extract. Hexane, petroleum ether, chloroform and ethanol extracts of the shade dried leaves were prepared by soxhelation and antiproliferative activity was assessed using human cancer cell lines of lung (A-549), colon (CoLo-05), ovary (IGR-OV-1), prostrate (PC3), leukaemia (THP-1) and breast (MCF-7) cancer. Bioactivity-derived fractionation was carried out for most active extract by column chromatography. The phytochemical studies indicated alkaloids, anthraquinones, terpenoids in the alcohol, chloroform extracts and tannins, terpenoids, reducing sugars in the petroleum ether and hexane extracts. Ethanol extract showed maximum inhibition in colon and breast carcinoma cell lines at a dose of 100 μg/ml. Column chromatography of the ethanol extract yielded five fractions. Out of this, fractions 2, 4 and 5 showed significant inhibition in leukaemia cell line with IC50 of 12.5, 86.2 and >100 μg/ml for fractions 2, 4 and 5, respectively. High-performance thin layer chromatography of the fraction 2 revealed imperatorin as one of the major phytoconstituents. Among the different extracts investigated, ethanol extract exhibited significant antiproliferative activity and its fraction 2 containing furanocoumarin imperatorin showed antiproliferative activity against leukaemia cell line with IC50 of 12.5 μg/ml.

No MeSH data available.


Related in: MedlinePlus