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Pharmacognostic standardisation and antiproliferative activity of Aegle marmelos (L.) Correa leaves in various human cancer cell lines.

Bhatti R, Singh J, Saxena AK, Suri N, Ishar MP - Indian J Pharm Sci (2013)

Bottom Line: The phytochemical studies indicated alkaloids, anthraquinones, terpenoids in the alcohol, chloroform extracts and tannins, terpenoids, reducing sugars in the petroleum ether and hexane extracts.Out of this, fractions 2, 4 and 5 showed significant inhibition in leukaemia cell line with IC50 of 12.5, 86.2 and >100 μg/ml for fractions 2, 4 and 5, respectively.Among the different extracts investigated, ethanol extract exhibited significant antiproliferative activity and its fraction 2 containing furanocoumarin imperatorin showed antiproliferative activity against leukaemia cell line with IC50 of 12.5 μg/ml.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Sciences, Guru Nanak Dev University, Amritsar-143 005, India.

ABSTRACT
Therapeutic management of cancer is a great clinical challenge and alternative medicines are being extensively explored to have integrated approach to cure cancer. Aegle marmelos (L.) Correa (Rutaceae) is known for its hypoglycaemic, radioprotective, antidiarrhoeal and many other pharmacological activities. The present study is designed to carryout pharmacognostic standardisation and evaluation of antiproliferative activity of the leaf extracts Aegle marmelos (L.) Correa (Rutaceae) and the chromatographic fractions of the most active extract. Hexane, petroleum ether, chloroform and ethanol extracts of the shade dried leaves were prepared by soxhelation and antiproliferative activity was assessed using human cancer cell lines of lung (A-549), colon (CoLo-05), ovary (IGR-OV-1), prostrate (PC3), leukaemia (THP-1) and breast (MCF-7) cancer. Bioactivity-derived fractionation was carried out for most active extract by column chromatography. The phytochemical studies indicated alkaloids, anthraquinones, terpenoids in the alcohol, chloroform extracts and tannins, terpenoids, reducing sugars in the petroleum ether and hexane extracts. Ethanol extract showed maximum inhibition in colon and breast carcinoma cell lines at a dose of 100 μg/ml. Column chromatography of the ethanol extract yielded five fractions. Out of this, fractions 2, 4 and 5 showed significant inhibition in leukaemia cell line with IC50 of 12.5, 86.2 and >100 μg/ml for fractions 2, 4 and 5, respectively. High-performance thin layer chromatography of the fraction 2 revealed imperatorin as one of the major phytoconstituents. Among the different extracts investigated, ethanol extract exhibited significant antiproliferative activity and its fraction 2 containing furanocoumarin imperatorin showed antiproliferative activity against leukaemia cell line with IC50 of 12.5 μg/ml.

No MeSH data available.


Related in: MedlinePlus

Trichome morphology.(a) Non-glandular covering trichomes on the midrib portion of the leaf fragment. (b) A covering trichome under low magnification. (c) A covering trichome (enlarged). [MR- midrib, St- stoma, Tr- trichome].
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Figure 3: Trichome morphology.(a) Non-glandular covering trichomes on the midrib portion of the leaf fragment. (b) A covering trichome under low magnification. (c) A covering trichome (enlarged). [MR- midrib, St- stoma, Tr- trichome].

Mentions: Fragments of epidermal layer with angular, polyhedral epidermal cells with narrow lumen, thin and straight walls are seen in the powder (fig. 2). Cyclocytic stomata, with each stoma having a ring of four cells encircling the guard cells or a second ring of subsidiary cells, are seen on the epidermis (fig. 3). Some of the epidermal cells are secretory cells, which produce aromatic oil compounds and contain dense cytoplasm that stains deeply (fig. 2b). Non-glandular epidermal trichomes, 150-200 μm long and 10 μm thick, unicellular, unbranched, curved or less commonly straight having thick walls, wide lumen and smooth surface are common in the powder and occur mostly along the veins of the leaf fragments (fig. 2a–c).


Pharmacognostic standardisation and antiproliferative activity of Aegle marmelos (L.) Correa leaves in various human cancer cell lines.

Bhatti R, Singh J, Saxena AK, Suri N, Ishar MP - Indian J Pharm Sci (2013)

Trichome morphology.(a) Non-glandular covering trichomes on the midrib portion of the leaf fragment. (b) A covering trichome under low magnification. (c) A covering trichome (enlarged). [MR- midrib, St- stoma, Tr- trichome].
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3928725&req=5

Figure 3: Trichome morphology.(a) Non-glandular covering trichomes on the midrib portion of the leaf fragment. (b) A covering trichome under low magnification. (c) A covering trichome (enlarged). [MR- midrib, St- stoma, Tr- trichome].
Mentions: Fragments of epidermal layer with angular, polyhedral epidermal cells with narrow lumen, thin and straight walls are seen in the powder (fig. 2). Cyclocytic stomata, with each stoma having a ring of four cells encircling the guard cells or a second ring of subsidiary cells, are seen on the epidermis (fig. 3). Some of the epidermal cells are secretory cells, which produce aromatic oil compounds and contain dense cytoplasm that stains deeply (fig. 2b). Non-glandular epidermal trichomes, 150-200 μm long and 10 μm thick, unicellular, unbranched, curved or less commonly straight having thick walls, wide lumen and smooth surface are common in the powder and occur mostly along the veins of the leaf fragments (fig. 2a–c).

Bottom Line: The phytochemical studies indicated alkaloids, anthraquinones, terpenoids in the alcohol, chloroform extracts and tannins, terpenoids, reducing sugars in the petroleum ether and hexane extracts.Out of this, fractions 2, 4 and 5 showed significant inhibition in leukaemia cell line with IC50 of 12.5, 86.2 and >100 μg/ml for fractions 2, 4 and 5, respectively.Among the different extracts investigated, ethanol extract exhibited significant antiproliferative activity and its fraction 2 containing furanocoumarin imperatorin showed antiproliferative activity against leukaemia cell line with IC50 of 12.5 μg/ml.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Sciences, Guru Nanak Dev University, Amritsar-143 005, India.

ABSTRACT
Therapeutic management of cancer is a great clinical challenge and alternative medicines are being extensively explored to have integrated approach to cure cancer. Aegle marmelos (L.) Correa (Rutaceae) is known for its hypoglycaemic, radioprotective, antidiarrhoeal and many other pharmacological activities. The present study is designed to carryout pharmacognostic standardisation and evaluation of antiproliferative activity of the leaf extracts Aegle marmelos (L.) Correa (Rutaceae) and the chromatographic fractions of the most active extract. Hexane, petroleum ether, chloroform and ethanol extracts of the shade dried leaves were prepared by soxhelation and antiproliferative activity was assessed using human cancer cell lines of lung (A-549), colon (CoLo-05), ovary (IGR-OV-1), prostrate (PC3), leukaemia (THP-1) and breast (MCF-7) cancer. Bioactivity-derived fractionation was carried out for most active extract by column chromatography. The phytochemical studies indicated alkaloids, anthraquinones, terpenoids in the alcohol, chloroform extracts and tannins, terpenoids, reducing sugars in the petroleum ether and hexane extracts. Ethanol extract showed maximum inhibition in colon and breast carcinoma cell lines at a dose of 100 μg/ml. Column chromatography of the ethanol extract yielded five fractions. Out of this, fractions 2, 4 and 5 showed significant inhibition in leukaemia cell line with IC50 of 12.5, 86.2 and >100 μg/ml for fractions 2, 4 and 5, respectively. High-performance thin layer chromatography of the fraction 2 revealed imperatorin as one of the major phytoconstituents. Among the different extracts investigated, ethanol extract exhibited significant antiproliferative activity and its fraction 2 containing furanocoumarin imperatorin showed antiproliferative activity against leukaemia cell line with IC50 of 12.5 μg/ml.

No MeSH data available.


Related in: MedlinePlus