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A CCD-based reader combined with CdS quantum dot-labeled lateral flow strips for ultrasensitive quantitative detection of CagA.

Gui C, Wang K, Li C, Dai X, Cui D - Nanoscale Res Lett (2014)

Bottom Line: CagA is proved to be associated closely with initiation of gastric carcinoma.A revised weighted threshold histogram equalization (WTHE) image processing algorithm was applied to analyze the result.CdS quantum dot-labeled lateral flow strips for detection of CagA were prepared.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Nano Biomedicine and Engineering, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Institute of Micro/Nano Science and Technology, Shanghai Jiao Tong University, 800Dongchuan Road, Shanghai 200240, People's Republic of China. wk_xa@163.com.

ABSTRACT
Immunochromatographic assays are widely used to detect many analytes. CagA is proved to be associated closely with initiation of gastric carcinoma. Here, we reported that a charge-coupled device (CCD)-based test strip reader combined with CdS quantum dot-labeled lateral flow strips for quantitative detection of CagA was developed, which used 365-nm ultraviolet LED as the excitation light source, and captured the test strip images through an acquisition module. Then, the captured image was transferred to the computer and was processed by a software system. A revised weighted threshold histogram equalization (WTHE) image processing algorithm was applied to analyze the result. CdS quantum dot-labeled lateral flow strips for detection of CagA were prepared. One hundred sera samples from clinical patients with gastric cancer and healthy people were prepared for detection, which demonstrated that the device could realize rapid, stable, and point-of-care detection, with a sensitivity of 20 pg/mL.

No MeSH data available.


Related in: MedlinePlus

Curve figure of the test strip image after processing of the proposed modified WTHE algorithm.
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Figure 8: Curve figure of the test strip image after processing of the proposed modified WTHE algorithm.

Mentions: During processing, we set v = 0.1 and r = 0.4, with the least mean square error (MSE). After image processing, the distinct graph of T-line and C-line is displayed in Figure 8 to certify the effectiveness of this algorithm.


A CCD-based reader combined with CdS quantum dot-labeled lateral flow strips for ultrasensitive quantitative detection of CagA.

Gui C, Wang K, Li C, Dai X, Cui D - Nanoscale Res Lett (2014)

Curve figure of the test strip image after processing of the proposed modified WTHE algorithm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3928623&req=5

Figure 8: Curve figure of the test strip image after processing of the proposed modified WTHE algorithm.
Mentions: During processing, we set v = 0.1 and r = 0.4, with the least mean square error (MSE). After image processing, the distinct graph of T-line and C-line is displayed in Figure 8 to certify the effectiveness of this algorithm.

Bottom Line: CagA is proved to be associated closely with initiation of gastric carcinoma.A revised weighted threshold histogram equalization (WTHE) image processing algorithm was applied to analyze the result.CdS quantum dot-labeled lateral flow strips for detection of CagA were prepared.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Nano Biomedicine and Engineering, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Institute of Micro/Nano Science and Technology, Shanghai Jiao Tong University, 800Dongchuan Road, Shanghai 200240, People's Republic of China. wk_xa@163.com.

ABSTRACT
Immunochromatographic assays are widely used to detect many analytes. CagA is proved to be associated closely with initiation of gastric carcinoma. Here, we reported that a charge-coupled device (CCD)-based test strip reader combined with CdS quantum dot-labeled lateral flow strips for quantitative detection of CagA was developed, which used 365-nm ultraviolet LED as the excitation light source, and captured the test strip images through an acquisition module. Then, the captured image was transferred to the computer and was processed by a software system. A revised weighted threshold histogram equalization (WTHE) image processing algorithm was applied to analyze the result. CdS quantum dot-labeled lateral flow strips for detection of CagA were prepared. One hundred sera samples from clinical patients with gastric cancer and healthy people were prepared for detection, which demonstrated that the device could realize rapid, stable, and point-of-care detection, with a sensitivity of 20 pg/mL.

No MeSH data available.


Related in: MedlinePlus