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A CCD-based reader combined with CdS quantum dot-labeled lateral flow strips for ultrasensitive quantitative detection of CagA.

Gui C, Wang K, Li C, Dai X, Cui D - Nanoscale Res Lett (2014)

Bottom Line: CagA is proved to be associated closely with initiation of gastric carcinoma.A revised weighted threshold histogram equalization (WTHE) image processing algorithm was applied to analyze the result.CdS quantum dot-labeled lateral flow strips for detection of CagA were prepared.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Nano Biomedicine and Engineering, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Institute of Micro/Nano Science and Technology, Shanghai Jiao Tong University, 800Dongchuan Road, Shanghai 200240, People's Republic of China. wk_xa@163.com.

ABSTRACT
Immunochromatographic assays are widely used to detect many analytes. CagA is proved to be associated closely with initiation of gastric carcinoma. Here, we reported that a charge-coupled device (CCD)-based test strip reader combined with CdS quantum dot-labeled lateral flow strips for quantitative detection of CagA was developed, which used 365-nm ultraviolet LED as the excitation light source, and captured the test strip images through an acquisition module. Then, the captured image was transferred to the computer and was processed by a software system. A revised weighted threshold histogram equalization (WTHE) image processing algorithm was applied to analyze the result. CdS quantum dot-labeled lateral flow strips for detection of CagA were prepared. One hundred sera samples from clinical patients with gastric cancer and healthy people were prepared for detection, which demonstrated that the device could realize rapid, stable, and point-of-care detection, with a sensitivity of 20 pg/mL.

No MeSH data available.


Related in: MedlinePlus

Hardware units of the device. (a) CCD image sensor. (b) UV filter. (c) Excitation light source. (d) Integrated instrument.
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Figure 6: Hardware units of the device. (a) CCD image sensor. (b) UV filter. (c) Excitation light source. (d) Integrated instrument.

Mentions: Figure 6 shows each component of the device. Figure 6a shows the CCD image sensor with a volume of 29 × 29 × 29 m3. Figure 6c,d represents the excitation light source and the integrated instrument, respectively. Compared with the use of the card acquisition card, that of the CCD image sensor with a USB is more convenient and has less cost. The UV filter is displayed in Figure 6b and could be connected with the CCD image sensor, playing an important role in eliminating interference of the light source. In addition, employing a lithium battery made the device viable without power supply for more than 6 h.


A CCD-based reader combined with CdS quantum dot-labeled lateral flow strips for ultrasensitive quantitative detection of CagA.

Gui C, Wang K, Li C, Dai X, Cui D - Nanoscale Res Lett (2014)

Hardware units of the device. (a) CCD image sensor. (b) UV filter. (c) Excitation light source. (d) Integrated instrument.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3928623&req=5

Figure 6: Hardware units of the device. (a) CCD image sensor. (b) UV filter. (c) Excitation light source. (d) Integrated instrument.
Mentions: Figure 6 shows each component of the device. Figure 6a shows the CCD image sensor with a volume of 29 × 29 × 29 m3. Figure 6c,d represents the excitation light source and the integrated instrument, respectively. Compared with the use of the card acquisition card, that of the CCD image sensor with a USB is more convenient and has less cost. The UV filter is displayed in Figure 6b and could be connected with the CCD image sensor, playing an important role in eliminating interference of the light source. In addition, employing a lithium battery made the device viable without power supply for more than 6 h.

Bottom Line: CagA is proved to be associated closely with initiation of gastric carcinoma.A revised weighted threshold histogram equalization (WTHE) image processing algorithm was applied to analyze the result.CdS quantum dot-labeled lateral flow strips for detection of CagA were prepared.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Nano Biomedicine and Engineering, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Institute of Micro/Nano Science and Technology, Shanghai Jiao Tong University, 800Dongchuan Road, Shanghai 200240, People's Republic of China. wk_xa@163.com.

ABSTRACT
Immunochromatographic assays are widely used to detect many analytes. CagA is proved to be associated closely with initiation of gastric carcinoma. Here, we reported that a charge-coupled device (CCD)-based test strip reader combined with CdS quantum dot-labeled lateral flow strips for quantitative detection of CagA was developed, which used 365-nm ultraviolet LED as the excitation light source, and captured the test strip images through an acquisition module. Then, the captured image was transferred to the computer and was processed by a software system. A revised weighted threshold histogram equalization (WTHE) image processing algorithm was applied to analyze the result. CdS quantum dot-labeled lateral flow strips for detection of CagA were prepared. One hundred sera samples from clinical patients with gastric cancer and healthy people were prepared for detection, which demonstrated that the device could realize rapid, stable, and point-of-care detection, with a sensitivity of 20 pg/mL.

No MeSH data available.


Related in: MedlinePlus