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Decolourisation of Different Dyes by two Pseudomonas Strains Under Various Growth Conditions.

Zabłocka-Godlewska E, Przystaś W, Grabińska-Sota E - Water Air Soil Pollut (2014)

Bottom Line: The best results were reached in shaken and semistatic samples (exception Evans blue).In most cases, dyes were removed faster and better by strain Sz6 than SDz3.At the end of the experiment, in majority of the samples, decrease of phyto- and zootoxicity was observed.

View Article: PubMed Central - PubMed

Affiliation: Environmental Biotechnology Department, Silesian University of Technology, Akademicka 2A, 44-100 Gliwice, Poland.

ABSTRACT
The aim of the present study was the decolourisation of mixture of two dyes belonging to different groups by two Pseudomonas fluorescens strains (Sz6 and SDz3). Influence of different incubation conditions on decolourisation effectiveness was evaluated. Dyes used in the experiment were diazo Evans blue (EB) and triphenylmethane brilliant green (BG). Another goal of the experiment was the estimation of toxicity of process by-products. Incubation conditions had a significant influence on the rate of decolourisation. The best results were reached in shaken and semistatic samples (exception Evans blue). After 24 h of experiment in semistatic conditions, BG removal reached up to 95.4 %, EB 72.8 % and dyes mixture 88.9 %. After 120 h, all tested dyes were completely removed. In most cases, dyes were removed faster and better by strain Sz6 than SDz3. At the end of the experiment, in majority of the samples, decrease of phyto- and zootoxicity was observed.

No MeSH data available.


Related in: MedlinePlus

UV-Visible spectral scans
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Fig4: UV-Visible spectral scans

Mentions: Dead biomass was used for evaluation of sorption (Fig. 3). Sorption of dyes mixture by Sz6 was approximately 9 %. The best results of dye sorption were noticed in samples with dyes mixture with strain SDz3 (from 44 % after 1 h up to 58.4 % dye removal after 120 h). As it is visible on Fig. 4, interaction between azo Evans blue and triphenylmethane brilliant green in mixture was observed. UV-Visible spectral scans (Fig. 4) show the displacement of major peak to 591 nm. Probably, this phenomenon caused higher sorption of dyes mixture by strain SDz3. These results were surprising because so high sorption was not observed in sample with individual dyes. For strain SDz3, sorption of brilliant green after 120 h was approximately 20 %, Evans blue approximately 10 % and for stain Sz6 approximately 8 and 12 %, respectively. Explanation of this phenomenon observed for dye mixture is difficult. Maybe it is connected with differences in quality and quantity of exopolysaccharides exuded by both tested strains what may influence on dyes sorption abilities when interaction between dyes happens. Wu et al. (2009) reported high effectiveness of dye removal by Pseudomonas otitidis WL-13 connected with adsorption on the biomass. Strain removed 95 % of brilliant green and malachite green in concentration 500 μmol l−1 after 12 h.Fig. 3


Decolourisation of Different Dyes by two Pseudomonas Strains Under Various Growth Conditions.

Zabłocka-Godlewska E, Przystaś W, Grabińska-Sota E - Water Air Soil Pollut (2014)

UV-Visible spectral scans
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3928507&req=5

Fig4: UV-Visible spectral scans
Mentions: Dead biomass was used for evaluation of sorption (Fig. 3). Sorption of dyes mixture by Sz6 was approximately 9 %. The best results of dye sorption were noticed in samples with dyes mixture with strain SDz3 (from 44 % after 1 h up to 58.4 % dye removal after 120 h). As it is visible on Fig. 4, interaction between azo Evans blue and triphenylmethane brilliant green in mixture was observed. UV-Visible spectral scans (Fig. 4) show the displacement of major peak to 591 nm. Probably, this phenomenon caused higher sorption of dyes mixture by strain SDz3. These results were surprising because so high sorption was not observed in sample with individual dyes. For strain SDz3, sorption of brilliant green after 120 h was approximately 20 %, Evans blue approximately 10 % and for stain Sz6 approximately 8 and 12 %, respectively. Explanation of this phenomenon observed for dye mixture is difficult. Maybe it is connected with differences in quality and quantity of exopolysaccharides exuded by both tested strains what may influence on dyes sorption abilities when interaction between dyes happens. Wu et al. (2009) reported high effectiveness of dye removal by Pseudomonas otitidis WL-13 connected with adsorption on the biomass. Strain removed 95 % of brilliant green and malachite green in concentration 500 μmol l−1 after 12 h.Fig. 3

Bottom Line: The best results were reached in shaken and semistatic samples (exception Evans blue).In most cases, dyes were removed faster and better by strain Sz6 than SDz3.At the end of the experiment, in majority of the samples, decrease of phyto- and zootoxicity was observed.

View Article: PubMed Central - PubMed

Affiliation: Environmental Biotechnology Department, Silesian University of Technology, Akademicka 2A, 44-100 Gliwice, Poland.

ABSTRACT
The aim of the present study was the decolourisation of mixture of two dyes belonging to different groups by two Pseudomonas fluorescens strains (Sz6 and SDz3). Influence of different incubation conditions on decolourisation effectiveness was evaluated. Dyes used in the experiment were diazo Evans blue (EB) and triphenylmethane brilliant green (BG). Another goal of the experiment was the estimation of toxicity of process by-products. Incubation conditions had a significant influence on the rate of decolourisation. The best results were reached in shaken and semistatic samples (exception Evans blue). After 24 h of experiment in semistatic conditions, BG removal reached up to 95.4 %, EB 72.8 % and dyes mixture 88.9 %. After 120 h, all tested dyes were completely removed. In most cases, dyes were removed faster and better by strain Sz6 than SDz3. At the end of the experiment, in majority of the samples, decrease of phyto- and zootoxicity was observed.

No MeSH data available.


Related in: MedlinePlus