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Virgin olive oil blended polyurethane micro/nanofibers ornamented with copper oxide nanocrystals for biomedical applications.

Amna T, Hassan MS, Yang J, Khil MS, Song KD, Oh JD, Hwang I - Int J Nanomedicine (2014)

Bottom Line: The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay results and SEM observation showed that the hybrid micronanofibrous scaffold was noncytotoxic to fibroblast cell culture and was found to benefit cell attachment and proliferation.Copper oxide-olive oil/PU wound dressing may exert its positive beneficial effects at every stage during wound-healing progression, and these micronanofibers may serve diverse biomedical applications, such as tissue regeneration, damaged skin treatment, wound healing applications, etc.Conclusively, the fabricated olive oil-copper oxide/PU micronanofibers combine the benefits of virgin olive oil and copper oxide, and therefore hold great promise for biomedical applications in the near future.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Sciences and Biotechnology, Jeonju, South Korea.

ABSTRACT
Recently, substantial interest has been generated in using electrospun biomimetic nanofibers of hybrids, particularly organic/inorganic, to engineer different tissues. The present work, for the first time, introduced a unique natural and synthetic hybrid micronanofiber wound dressing, composed of virgin olive oil/copper oxide nanocrystals and polyurethane (PU), developed via facile electrospinning. The as-spun organic/inorganic hybrid micronanofibers were characterized by scanning electron microscopy (SEM), energy dispersive X-ray analysis, X-ray diffraction, electron probe microanalysis, and transmission electron microscopy. The interaction of cells with scaffold was studied by culturing NIH 3T3 fibroblasts on an as-spun hybrid micronanofibrous mat, and viability, proliferation, and growth were assessed. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay results and SEM observation showed that the hybrid micronanofibrous scaffold was noncytotoxic to fibroblast cell culture and was found to benefit cell attachment and proliferation. Hence our results suggest the potential utilization of as-spun micronanoscaffolds for tissue engineering. Copper oxide-olive oil/PU wound dressing may exert its positive beneficial effects at every stage during wound-healing progression, and these micronanofibers may serve diverse biomedical applications, such as tissue regeneration, damaged skin treatment, wound healing applications, etc. Conclusively, the fabricated olive oil-copper oxide/PU micronanofibers combine the benefits of virgin olive oil and copper oxide, and therefore hold great promise for biomedical applications in the near future.

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Related in: MedlinePlus

UV-DRS spectra of olive oil/CuO–PU hybrid mat before and after incubation in PBS (A), and a digital photograph of hybrid mat before and after incubation (B).Note: The inset shows a picture of a hybrid mat in PBS for incubation (B).Abbreviations: CuO, copper oxide; PBS, phosphate buffered saline; PU, polyurethane; UV-DRS, ultraviolet-diffuse reflectance spectroscopic.
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f7-ijn-9-891: UV-DRS spectra of olive oil/CuO–PU hybrid mat before and after incubation in PBS (A), and a digital photograph of hybrid mat before and after incubation (B).Note: The inset shows a picture of a hybrid mat in PBS for incubation (B).Abbreviations: CuO, copper oxide; PBS, phosphate buffered saline; PU, polyurethane; UV-DRS, ultraviolet-diffuse reflectance spectroscopic.

Mentions: The cytobiocompatibility of as-spun organic/inorganic hybrid micronanofibers was investigated using fibroblasts. Figure 6A shows the MTT assay results after specific incubation times. No toxic effects were seen in the fibroblasts cultured on hybrid micronanofibers and untreated control. Cell numbers increased exponentially during the culture period, and were active throughout the incubation time. The morphology and spreading behavior of fibroblasts on the hybrid miconanofibers were analyzed by SEM at the end of incubation, and results (Figure 6B) confirm that the cells maintained their morphology and growth pattern. Additionally we performed a series of wash experiments to evaluate CuO migration from the micronanofibrous mats using UV-DRS. There was no change in the UV-DRS spectra of mats before and after incubation (Figure 7A). Also no significant change in the color of the mat was observed before and after incubation (Figure 7B). These findings clearly indicate that there is no, or negligible, CuO migration from the micronanofibrous mats. This is in accord with reports in the literature that copper is a long-lasting bactericide, and the release of copper is very slow and in very small quantities.26,27 Conclusively, our synthesized nanomatrix did not inhibit cell proliferation, and confluent cell growth was observed at the end of the culture period.


Virgin olive oil blended polyurethane micro/nanofibers ornamented with copper oxide nanocrystals for biomedical applications.

Amna T, Hassan MS, Yang J, Khil MS, Song KD, Oh JD, Hwang I - Int J Nanomedicine (2014)

UV-DRS spectra of olive oil/CuO–PU hybrid mat before and after incubation in PBS (A), and a digital photograph of hybrid mat before and after incubation (B).Note: The inset shows a picture of a hybrid mat in PBS for incubation (B).Abbreviations: CuO, copper oxide; PBS, phosphate buffered saline; PU, polyurethane; UV-DRS, ultraviolet-diffuse reflectance spectroscopic.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3928461&req=5

f7-ijn-9-891: UV-DRS spectra of olive oil/CuO–PU hybrid mat before and after incubation in PBS (A), and a digital photograph of hybrid mat before and after incubation (B).Note: The inset shows a picture of a hybrid mat in PBS for incubation (B).Abbreviations: CuO, copper oxide; PBS, phosphate buffered saline; PU, polyurethane; UV-DRS, ultraviolet-diffuse reflectance spectroscopic.
Mentions: The cytobiocompatibility of as-spun organic/inorganic hybrid micronanofibers was investigated using fibroblasts. Figure 6A shows the MTT assay results after specific incubation times. No toxic effects were seen in the fibroblasts cultured on hybrid micronanofibers and untreated control. Cell numbers increased exponentially during the culture period, and were active throughout the incubation time. The morphology and spreading behavior of fibroblasts on the hybrid miconanofibers were analyzed by SEM at the end of incubation, and results (Figure 6B) confirm that the cells maintained their morphology and growth pattern. Additionally we performed a series of wash experiments to evaluate CuO migration from the micronanofibrous mats using UV-DRS. There was no change in the UV-DRS spectra of mats before and after incubation (Figure 7A). Also no significant change in the color of the mat was observed before and after incubation (Figure 7B). These findings clearly indicate that there is no, or negligible, CuO migration from the micronanofibrous mats. This is in accord with reports in the literature that copper is a long-lasting bactericide, and the release of copper is very slow and in very small quantities.26,27 Conclusively, our synthesized nanomatrix did not inhibit cell proliferation, and confluent cell growth was observed at the end of the culture period.

Bottom Line: The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay results and SEM observation showed that the hybrid micronanofibrous scaffold was noncytotoxic to fibroblast cell culture and was found to benefit cell attachment and proliferation.Copper oxide-olive oil/PU wound dressing may exert its positive beneficial effects at every stage during wound-healing progression, and these micronanofibers may serve diverse biomedical applications, such as tissue regeneration, damaged skin treatment, wound healing applications, etc.Conclusively, the fabricated olive oil-copper oxide/PU micronanofibers combine the benefits of virgin olive oil and copper oxide, and therefore hold great promise for biomedical applications in the near future.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Sciences and Biotechnology, Jeonju, South Korea.

ABSTRACT
Recently, substantial interest has been generated in using electrospun biomimetic nanofibers of hybrids, particularly organic/inorganic, to engineer different tissues. The present work, for the first time, introduced a unique natural and synthetic hybrid micronanofiber wound dressing, composed of virgin olive oil/copper oxide nanocrystals and polyurethane (PU), developed via facile electrospinning. The as-spun organic/inorganic hybrid micronanofibers were characterized by scanning electron microscopy (SEM), energy dispersive X-ray analysis, X-ray diffraction, electron probe microanalysis, and transmission electron microscopy. The interaction of cells with scaffold was studied by culturing NIH 3T3 fibroblasts on an as-spun hybrid micronanofibrous mat, and viability, proliferation, and growth were assessed. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay results and SEM observation showed that the hybrid micronanofibrous scaffold was noncytotoxic to fibroblast cell culture and was found to benefit cell attachment and proliferation. Hence our results suggest the potential utilization of as-spun micronanoscaffolds for tissue engineering. Copper oxide-olive oil/PU wound dressing may exert its positive beneficial effects at every stage during wound-healing progression, and these micronanofibers may serve diverse biomedical applications, such as tissue regeneration, damaged skin treatment, wound healing applications, etc. Conclusively, the fabricated olive oil-copper oxide/PU micronanofibers combine the benefits of virgin olive oil and copper oxide, and therefore hold great promise for biomedical applications in the near future.

Show MeSH
Related in: MedlinePlus