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Fucoidan reduces secretion and expression of vascular endothelial growth factor in the retinal pigment epithelium and reduces angiogenesis in vitro.

Dithmer M, Fuchs S, Shi Y, Schmidt H, Richert E, Roider J, Klettner A - PLoS ONE (2014)

Bottom Line: Fucoidan displays no toxicity and does not diminish proliferation or phagocytosis, but reduces wound healing in RPE cells.Fucoidan decreases VEGF secretion in RPE/choroid explants and RPE cells.In conclusion, fucoidan is a non-toxic agent that reduces VEGF expression and angiogenesis in vitro and may be of interest for further studies as a potential therapy against exudative age-related macular degeneration.

View Article: PubMed Central - PubMed

Affiliation: University of Kiel, University Medical Center, Department of Ophthalmology, Kiel, Germany.

ABSTRACT
Fucoidan is a polysaccharide isolated from brown algae which is of current interest for anti-tumor therapy. In this study, we investigated the effect of fucoidan on the retinal pigment epithelium (RPE), looking at physiology, vascular endothelial growth factor (VEGF) secretion, and angiogenesis, thus investigating a potential use of fucoidan for the treatment of exudative age-related macular degeneration. For this study, human RPE cell line ARPE-19 and primary porcine RPE cells were used, as well as RPE/choroid perfusion organ cultures. The effect of fucoidan on RPE cells was investigated with methyl thiazolyl tetrazolium--assay, trypan blue exclusion assay, phagocytosis assay and a wound healing assay. VEGF expression was evaluated in immunocytochemistry and Western blot, VEGF secretion was evaluated in ELISA. The effect of fucoidan on angiogenesis was tested in a Matrigel assay using calcein-AM vital staining, evaluated by confocal laser scanning microcopy and quantitative image analysis. Fucoidan displays no toxicity and does not diminish proliferation or phagocytosis, but reduces wound healing in RPE cells. Fucoidan decreases VEGF secretion in RPE/choroid explants and RPE cells. Furthermore, it diminishes VEGF expression in RPE cells even when co-applied with bevacizumab. Furthermore, fucoidan reduces RPE-supernatant- and VEGF-induced angiogenesis of peripheral endothelial cells. In conclusion, fucoidan is a non-toxic agent that reduces VEGF expression and angiogenesis in vitro and may be of interest for further studies as a potential therapy against exudative age-related macular degeneration.

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Phagocytosis.Primary RPE cells were stimulated with 100 µg/ml fucoidan for 1 hour. RPE cells were exposed to FITC-labeled, photoreceptor outer segment opsonized beads for 4 hours and uptake of the beads was evaluated in fluorescence microscope. No influence of fucoidan on RPE phagocytosis was found. A) control, B) fucoidan, C) quantification of uptaken beads. Significance was determined with student's t-test.
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pone-0089150-g002: Phagocytosis.Primary RPE cells were stimulated with 100 µg/ml fucoidan for 1 hour. RPE cells were exposed to FITC-labeled, photoreceptor outer segment opsonized beads for 4 hours and uptake of the beads was evaluated in fluorescence microscope. No influence of fucoidan on RPE phagocytosis was found. A) control, B) fucoidan, C) quantification of uptaken beads. Significance was determined with student's t-test.

Mentions: To analyze the effect of fucoidan on the phagocytosis of photoreceptor outer segments by primary RPE, a phagocytosis assay using POS-opsonized beads was conducted, which detects bound and internalized beads. No influence of fucoidan on the phagocytosis by the RPE could be found (control: 11.38 ± 3.63 beads/cells; 100 µg/ml fucoidan: 12.24±3.72 beads/cell) (Fig. 2).


Fucoidan reduces secretion and expression of vascular endothelial growth factor in the retinal pigment epithelium and reduces angiogenesis in vitro.

Dithmer M, Fuchs S, Shi Y, Schmidt H, Richert E, Roider J, Klettner A - PLoS ONE (2014)

Phagocytosis.Primary RPE cells were stimulated with 100 µg/ml fucoidan for 1 hour. RPE cells were exposed to FITC-labeled, photoreceptor outer segment opsonized beads for 4 hours and uptake of the beads was evaluated in fluorescence microscope. No influence of fucoidan on RPE phagocytosis was found. A) control, B) fucoidan, C) quantification of uptaken beads. Significance was determined with student's t-test.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3928431&req=5

pone-0089150-g002: Phagocytosis.Primary RPE cells were stimulated with 100 µg/ml fucoidan for 1 hour. RPE cells were exposed to FITC-labeled, photoreceptor outer segment opsonized beads for 4 hours and uptake of the beads was evaluated in fluorescence microscope. No influence of fucoidan on RPE phagocytosis was found. A) control, B) fucoidan, C) quantification of uptaken beads. Significance was determined with student's t-test.
Mentions: To analyze the effect of fucoidan on the phagocytosis of photoreceptor outer segments by primary RPE, a phagocytosis assay using POS-opsonized beads was conducted, which detects bound and internalized beads. No influence of fucoidan on the phagocytosis by the RPE could be found (control: 11.38 ± 3.63 beads/cells; 100 µg/ml fucoidan: 12.24±3.72 beads/cell) (Fig. 2).

Bottom Line: Fucoidan displays no toxicity and does not diminish proliferation or phagocytosis, but reduces wound healing in RPE cells.Fucoidan decreases VEGF secretion in RPE/choroid explants and RPE cells.In conclusion, fucoidan is a non-toxic agent that reduces VEGF expression and angiogenesis in vitro and may be of interest for further studies as a potential therapy against exudative age-related macular degeneration.

View Article: PubMed Central - PubMed

Affiliation: University of Kiel, University Medical Center, Department of Ophthalmology, Kiel, Germany.

ABSTRACT
Fucoidan is a polysaccharide isolated from brown algae which is of current interest for anti-tumor therapy. In this study, we investigated the effect of fucoidan on the retinal pigment epithelium (RPE), looking at physiology, vascular endothelial growth factor (VEGF) secretion, and angiogenesis, thus investigating a potential use of fucoidan for the treatment of exudative age-related macular degeneration. For this study, human RPE cell line ARPE-19 and primary porcine RPE cells were used, as well as RPE/choroid perfusion organ cultures. The effect of fucoidan on RPE cells was investigated with methyl thiazolyl tetrazolium--assay, trypan blue exclusion assay, phagocytosis assay and a wound healing assay. VEGF expression was evaluated in immunocytochemistry and Western blot, VEGF secretion was evaluated in ELISA. The effect of fucoidan on angiogenesis was tested in a Matrigel assay using calcein-AM vital staining, evaluated by confocal laser scanning microcopy and quantitative image analysis. Fucoidan displays no toxicity and does not diminish proliferation or phagocytosis, but reduces wound healing in RPE cells. Fucoidan decreases VEGF secretion in RPE/choroid explants and RPE cells. Furthermore, it diminishes VEGF expression in RPE cells even when co-applied with bevacizumab. Furthermore, fucoidan reduces RPE-supernatant- and VEGF-induced angiogenesis of peripheral endothelial cells. In conclusion, fucoidan is a non-toxic agent that reduces VEGF expression and angiogenesis in vitro and may be of interest for further studies as a potential therapy against exudative age-related macular degeneration.

Show MeSH
Related in: MedlinePlus