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Discrimination of wild Paris based on near infrared spectroscopy and high performance liquid chromatography combined with multivariate analysis.

Zhao Y, Zhang J, Yuan T, Shen T, Li W, Yang S, Hou Y, Wang Y, Jin H - PLoS ONE (2014)

Bottom Line: The results of the contents of chemical components described differences among collections quantitatively.A multivariate statistical model of PLS-DA showed geographical origins of Paris had a much greater influence on Paris compared with species.The quality of Paris showed regional dependence.

View Article: PubMed Central - PubMed

Affiliation: Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming, Yunnan, the People's Republic of China.

ABSTRACT
Different geographical origins and species of Paris obtained from southwestern China were discriminated by near infrared (NIR) spectroscopy and high performance liquid chromatography (HPLC) combined with multivariate analysis. The NIR parameter settings were scanning (64 times), resolution (4 cm(-1)), scanning range (10,000 cm(-1)∼4000 cm(-1)) and parallel collection (3 times). NIR spectrum was optimized by TQ 8.6 software, and the ranges 7455∼6852 cm(-1) and 5973∼4007 cm(-1) were selected according to the spectrum standard deviation. The contents of polyphyllin I, polyphyllin II, polyphyllin VI, and polyphyllin VII and total steroid saponins were detected by HPLC. The contents of chemical components data matrix and spectrum data matrix were integrated and analyzed by partial least squares discriminant analysis (PLS-DA). From the PLS-DA model of NIR spectrum, Paris samples were separated into three groups according to the different geographical origins. The R(2)X and Q(2)Y described accumulative contribution rates were 99.50% and 94.03% of the total variance, respectively. The PLS-DA model according to 12 species of Paris described 99.62% of the variation in X and predicted 95.23% in Y. The results of the contents of chemical components described differences among collections quantitatively. A multivariate statistical model of PLS-DA showed geographical origins of Paris had a much greater influence on Paris compared with species. NIR and HPLC combined with multivariate analysis could discriminate different geographical origins and different species. The quality of Paris showed regional dependence.

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Chemical structures of polyphyllin I, II, VI and VII.
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pone-0089100-g001: Chemical structures of polyphyllin I, II, VI and VII.

Mentions: Paris, belonging to the family Liliaceae, contains about 24 species and mainly distributes in Europe and Eastern Asia. There are 22 species of Paris in China, and the diversity center of Paris is located in Southwest China [7]. The dried rhizome paridis is the main raw material of Chinese patent drugs “Yunnan Baiyao”, “GongXue Ning”, and “Jidesheng snake tablet” [8]. The phytochemistry research indicates that abundant active ingredients including steroidal saponins, flavonoids, fatty acid ester and endophytic fungi are in the dried rhizome of Paris, and steroid saponins such as polyphyllin I, polyphyllin II, polyphyllin VI, and polyphyllin VII are the most investigated ones [8], [9], [10], [11]. Their chemical structures are depicted in Figure 1. Modern pharmacology has demonstrated that polyphyllin has powerful pharmacological activities on stypticity, spermicide, homeostasis, analgesic, and as a potential anti-cancer drug for the functions of cytotoxicity and induction of apoptosis [12], [13], [14].


Discrimination of wild Paris based on near infrared spectroscopy and high performance liquid chromatography combined with multivariate analysis.

Zhao Y, Zhang J, Yuan T, Shen T, Li W, Yang S, Hou Y, Wang Y, Jin H - PLoS ONE (2014)

Chemical structures of polyphyllin I, II, VI and VII.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3928364&req=5

pone-0089100-g001: Chemical structures of polyphyllin I, II, VI and VII.
Mentions: Paris, belonging to the family Liliaceae, contains about 24 species and mainly distributes in Europe and Eastern Asia. There are 22 species of Paris in China, and the diversity center of Paris is located in Southwest China [7]. The dried rhizome paridis is the main raw material of Chinese patent drugs “Yunnan Baiyao”, “GongXue Ning”, and “Jidesheng snake tablet” [8]. The phytochemistry research indicates that abundant active ingredients including steroidal saponins, flavonoids, fatty acid ester and endophytic fungi are in the dried rhizome of Paris, and steroid saponins such as polyphyllin I, polyphyllin II, polyphyllin VI, and polyphyllin VII are the most investigated ones [8], [9], [10], [11]. Their chemical structures are depicted in Figure 1. Modern pharmacology has demonstrated that polyphyllin has powerful pharmacological activities on stypticity, spermicide, homeostasis, analgesic, and as a potential anti-cancer drug for the functions of cytotoxicity and induction of apoptosis [12], [13], [14].

Bottom Line: The results of the contents of chemical components described differences among collections quantitatively.A multivariate statistical model of PLS-DA showed geographical origins of Paris had a much greater influence on Paris compared with species.The quality of Paris showed regional dependence.

View Article: PubMed Central - PubMed

Affiliation: Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming, Yunnan, the People's Republic of China.

ABSTRACT
Different geographical origins and species of Paris obtained from southwestern China were discriminated by near infrared (NIR) spectroscopy and high performance liquid chromatography (HPLC) combined with multivariate analysis. The NIR parameter settings were scanning (64 times), resolution (4 cm(-1)), scanning range (10,000 cm(-1)∼4000 cm(-1)) and parallel collection (3 times). NIR spectrum was optimized by TQ 8.6 software, and the ranges 7455∼6852 cm(-1) and 5973∼4007 cm(-1) were selected according to the spectrum standard deviation. The contents of polyphyllin I, polyphyllin II, polyphyllin VI, and polyphyllin VII and total steroid saponins were detected by HPLC. The contents of chemical components data matrix and spectrum data matrix were integrated and analyzed by partial least squares discriminant analysis (PLS-DA). From the PLS-DA model of NIR spectrum, Paris samples were separated into three groups according to the different geographical origins. The R(2)X and Q(2)Y described accumulative contribution rates were 99.50% and 94.03% of the total variance, respectively. The PLS-DA model according to 12 species of Paris described 99.62% of the variation in X and predicted 95.23% in Y. The results of the contents of chemical components described differences among collections quantitatively. A multivariate statistical model of PLS-DA showed geographical origins of Paris had a much greater influence on Paris compared with species. NIR and HPLC combined with multivariate analysis could discriminate different geographical origins and different species. The quality of Paris showed regional dependence.

Show MeSH
Related in: MedlinePlus