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Stem cell therapy and curcumin synergistically enhance recovery from spinal cord injury.

Ormond DR, Shannon C, Oppenheim J, Zeman R, Das K, Murali R, Jhanwar-Uniyal M - PLoS ONE (2014)

Bottom Line: Stem cell therapy improved recovery from moderate SCI, however, it had a limited effect on recovery after severe SCI.Curcumin stimulated NSC proliferation in vitro, and in combination with stem cell therapy, induced profound recovery from severe SCI as evidenced by improved functional locomotor recovery, increased body weight, and soleus muscle mass.Furthermore, our results indicate that the effect of curcumin extends beyond its known anti-inflammatory properties to the regulation of stem cell proliferation.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery, New York Medical College, Valhalla, New York, United States of America.

ABSTRACT
Acute traumatic spinal cord injury (SCI) is marked by the enhanced production of local cytokines and pro-inflammatory substances that induce gliosis and prevent reinnervation. The transplantation of stem cells is a promising treatment strategy for SCI. In order to facilitate functional recovery, we employed stem cell therapy alone or in combination with curcumin, a naturally-occurring anti-inflammatory component of turmeric (Curcuma longa), which potently inhibits NF-κB. Spinal cord contusion following laminectomy (T9-10) was performed using a weight drop apparatus (10 g over a 12.5 or 25 mm distance, representing moderate or severe SCI, respectively) in Sprague-Dawley rats. Neural stem cells (NSC) were isolated from subventricular zone (SVZ) and transplanted at the site of injury with or without curcumin treatment. Functional recovery was assessed by BBB score and body weight gain measured up to 6 weeks following SCI. At the conclusion of the study, the mass of soleus muscle was correlated with BBB score and body weight. Stem cell therapy improved recovery from moderate SCI, however, it had a limited effect on recovery after severe SCI. Curcumin stimulated NSC proliferation in vitro, and in combination with stem cell therapy, induced profound recovery from severe SCI as evidenced by improved functional locomotor recovery, increased body weight, and soleus muscle mass. These findings demonstrate that curcumin in conjunction with stem cell therapy synergistically improves recovery from severe SCI. Furthermore, our results indicate that the effect of curcumin extends beyond its known anti-inflammatory properties to the regulation of stem cell proliferation.

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Histopathological examination after severe SCI.A: NSC plus curcumin and NSC all displayed enhanced spared area relative to the total area suggesting an improvement in recovery. DMSO treated rats also demonstrated increased spared area of the spinal cord, but somewhat less than the NSC plus curcumin, or NSC treatments. Curcumin-treated rats demonstrated less spared area than the other three treatments. B: Photomicrographs of representative samples of spinal cord sections across the area of contusion stained with Lox blue.
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pone-0088916-g005: Histopathological examination after severe SCI.A: NSC plus curcumin and NSC all displayed enhanced spared area relative to the total area suggesting an improvement in recovery. DMSO treated rats also demonstrated increased spared area of the spinal cord, but somewhat less than the NSC plus curcumin, or NSC treatments. Curcumin-treated rats demonstrated less spared area than the other three treatments. B: Photomicrographs of representative samples of spinal cord sections across the area of contusion stained with Lox blue.

Mentions: Spared tissue in relation to the total area of NSC+Curcumin-, Curcumin-, and NSC-treated rats were presented along with control rats treated with DMSO (Fig. 5A). The rats treated with NSC+Curcumin and NSC monotherapy-treated rats showed better recovery as shown by the spared as compared to the total area.


Stem cell therapy and curcumin synergistically enhance recovery from spinal cord injury.

Ormond DR, Shannon C, Oppenheim J, Zeman R, Das K, Murali R, Jhanwar-Uniyal M - PLoS ONE (2014)

Histopathological examination after severe SCI.A: NSC plus curcumin and NSC all displayed enhanced spared area relative to the total area suggesting an improvement in recovery. DMSO treated rats also demonstrated increased spared area of the spinal cord, but somewhat less than the NSC plus curcumin, or NSC treatments. Curcumin-treated rats demonstrated less spared area than the other three treatments. B: Photomicrographs of representative samples of spinal cord sections across the area of contusion stained with Lox blue.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3928327&req=5

pone-0088916-g005: Histopathological examination after severe SCI.A: NSC plus curcumin and NSC all displayed enhanced spared area relative to the total area suggesting an improvement in recovery. DMSO treated rats also demonstrated increased spared area of the spinal cord, but somewhat less than the NSC plus curcumin, or NSC treatments. Curcumin-treated rats demonstrated less spared area than the other three treatments. B: Photomicrographs of representative samples of spinal cord sections across the area of contusion stained with Lox blue.
Mentions: Spared tissue in relation to the total area of NSC+Curcumin-, Curcumin-, and NSC-treated rats were presented along with control rats treated with DMSO (Fig. 5A). The rats treated with NSC+Curcumin and NSC monotherapy-treated rats showed better recovery as shown by the spared as compared to the total area.

Bottom Line: Stem cell therapy improved recovery from moderate SCI, however, it had a limited effect on recovery after severe SCI.Curcumin stimulated NSC proliferation in vitro, and in combination with stem cell therapy, induced profound recovery from severe SCI as evidenced by improved functional locomotor recovery, increased body weight, and soleus muscle mass.Furthermore, our results indicate that the effect of curcumin extends beyond its known anti-inflammatory properties to the regulation of stem cell proliferation.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery, New York Medical College, Valhalla, New York, United States of America.

ABSTRACT
Acute traumatic spinal cord injury (SCI) is marked by the enhanced production of local cytokines and pro-inflammatory substances that induce gliosis and prevent reinnervation. The transplantation of stem cells is a promising treatment strategy for SCI. In order to facilitate functional recovery, we employed stem cell therapy alone or in combination with curcumin, a naturally-occurring anti-inflammatory component of turmeric (Curcuma longa), which potently inhibits NF-κB. Spinal cord contusion following laminectomy (T9-10) was performed using a weight drop apparatus (10 g over a 12.5 or 25 mm distance, representing moderate or severe SCI, respectively) in Sprague-Dawley rats. Neural stem cells (NSC) were isolated from subventricular zone (SVZ) and transplanted at the site of injury with or without curcumin treatment. Functional recovery was assessed by BBB score and body weight gain measured up to 6 weeks following SCI. At the conclusion of the study, the mass of soleus muscle was correlated with BBB score and body weight. Stem cell therapy improved recovery from moderate SCI, however, it had a limited effect on recovery after severe SCI. Curcumin stimulated NSC proliferation in vitro, and in combination with stem cell therapy, induced profound recovery from severe SCI as evidenced by improved functional locomotor recovery, increased body weight, and soleus muscle mass. These findings demonstrate that curcumin in conjunction with stem cell therapy synergistically improves recovery from severe SCI. Furthermore, our results indicate that the effect of curcumin extends beyond its known anti-inflammatory properties to the regulation of stem cell proliferation.

Show MeSH
Related in: MedlinePlus