Limits...
Lack of association between mannose binding lectin and antibody responses after acellular pertussis vaccinations.

Gröndahl-Yli-Hannuksela K, Vuononvirta J, Peltola V, Mertsola J, He Q - PLoS ONE (2014)

Bottom Line: Follow-up was performed at 3, 5 and 10 years.MBL serum concentration was also measured from the adolescent cohort.No association was found with MBL2 exon 1 polymorphisms and antibody responses against vaccine antigens, after primary and booster dTpa vaccination.

View Article: PubMed Central - PubMed

Affiliation: Department of Infectious Disease Surveillance and Control, National Institute for Health and Welfare (THL), Turku, Finland.

ABSTRACT

Background: Mannose-binding lectin (MBL) is one of the key molecules in innate immunity and its role in human vaccine responses is poorly known. This study aimed to investigate the possible association of MBL polymorphisms with antibody production after primary and booster vaccinations with acellular pertussis vaccines in infants and adolescents.

Methodology/principal findings: Five hundred and sixty eight subjects were included in this study. In the adolescent cohort 355 subjects received a dose of diphtheria and tetanus toxoids and acellular pertussis (dTpa) vaccine ten years previously. Follow-up was performed at 3, 5 and 10 years. Infant cohort consisted of 213 subjects, who had received three primary doses of DTaP vaccine at 3, 5, and 12 months of age according to Finnish immunization program. Blood samples were collected before the vaccinations at 2,5 months of age and after the vaccinations at 13 months and 2 years of age. Concentrations of IgG antibodies to pertussis toxin, filamentous hemagglutinin, and pertactin and antibodies to diphtheria and tetanus toxoids were measured by standardized enzyme-linked immunosorbant assay. Single nucleotide polymorphisms of MBL2 gene exon1 (codons 52, 54, 57) were examined. MBL serum concentration was also measured from the adolescent cohort. No association was found with MBL2 exon 1 polymorphisms and antibody responses against vaccine antigens, after primary and booster dTpa vaccination.

Conclusions: This study indicates that MBL polymorphisms do not affect the production and persistence of antibodies after acellular pertussis vaccination. Our finding also suggests that MBL might not be involved in modulating antibody responses to the vaccines made of purified bacterial proteins.

Show MeSH

Related in: MedlinePlus

MBL2 genotypes; A/A, A/O and O/O, with the corresponding MBL concentrations in 355 adolescents (p<0.0001).A/A refers to wild type, A/O heterozygote variants and O/O homozygote variants, and O refers to exon1 variant alleles B, C or D. Statistical significance was calculated with Kruskal-Wallis test. The number of subjects in each group is shown. Data is represented with first and third quartile and median line. The ends of the whiskers represent maximum and minimum values of the data.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3928324&req=5

pone-0088919-g001: MBL2 genotypes; A/A, A/O and O/O, with the corresponding MBL concentrations in 355 adolescents (p<0.0001).A/A refers to wild type, A/O heterozygote variants and O/O homozygote variants, and O refers to exon1 variant alleles B, C or D. Statistical significance was calculated with Kruskal-Wallis test. The number of subjects in each group is shown. Data is represented with first and third quartile and median line. The ends of the whiskers represent maximum and minimum values of the data.

Mentions: Genotyping was successful in all subjects (355 adolescents and 213 infants). The distribution of MBL genotypes for both cohorts and MBL concentrations of the adolescent cohort are presented in Table 1 and Figure 1. The MBL concentration was significantly higher in subjects with wild type A/A than those with heterozygote variants A/O and with homozygote variants O/O. In subjects with heterozygote variants A/O, the MBL concentration was significantly higher than in those with homozygote variants O/O. There was no difference in the frequencies of MBL2 genotypes between genders (P = 0.91 and P = 0.67, for adolescent and infant cohort, respectively). No difference was observed in the MBL concentrations between genders in the adolescent group (p = 0.40). There was no correlation between MBL concentration and antibody response against any vaccine antigens in adolescents' cohort after first booster vaccine (for pertussis toxin R2 = 0.0004, Figure 2.). In the adolescent cohort, 15 (4.2%) subjects were found to have severe MBL deficiency (<50 ng/ml). As expected, the MBL concentrations are reduced in homozygote variants compared with wild type (Figure 1.) Due to this expected reduction and as no difference was observed in antibody concentrations, MBL concentration was not measured from the infant cohort.


Lack of association between mannose binding lectin and antibody responses after acellular pertussis vaccinations.

Gröndahl-Yli-Hannuksela K, Vuononvirta J, Peltola V, Mertsola J, He Q - PLoS ONE (2014)

MBL2 genotypes; A/A, A/O and O/O, with the corresponding MBL concentrations in 355 adolescents (p<0.0001).A/A refers to wild type, A/O heterozygote variants and O/O homozygote variants, and O refers to exon1 variant alleles B, C or D. Statistical significance was calculated with Kruskal-Wallis test. The number of subjects in each group is shown. Data is represented with first and third quartile and median line. The ends of the whiskers represent maximum and minimum values of the data.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3928324&req=5

pone-0088919-g001: MBL2 genotypes; A/A, A/O and O/O, with the corresponding MBL concentrations in 355 adolescents (p<0.0001).A/A refers to wild type, A/O heterozygote variants and O/O homozygote variants, and O refers to exon1 variant alleles B, C or D. Statistical significance was calculated with Kruskal-Wallis test. The number of subjects in each group is shown. Data is represented with first and third quartile and median line. The ends of the whiskers represent maximum and minimum values of the data.
Mentions: Genotyping was successful in all subjects (355 adolescents and 213 infants). The distribution of MBL genotypes for both cohorts and MBL concentrations of the adolescent cohort are presented in Table 1 and Figure 1. The MBL concentration was significantly higher in subjects with wild type A/A than those with heterozygote variants A/O and with homozygote variants O/O. In subjects with heterozygote variants A/O, the MBL concentration was significantly higher than in those with homozygote variants O/O. There was no difference in the frequencies of MBL2 genotypes between genders (P = 0.91 and P = 0.67, for adolescent and infant cohort, respectively). No difference was observed in the MBL concentrations between genders in the adolescent group (p = 0.40). There was no correlation between MBL concentration and antibody response against any vaccine antigens in adolescents' cohort after first booster vaccine (for pertussis toxin R2 = 0.0004, Figure 2.). In the adolescent cohort, 15 (4.2%) subjects were found to have severe MBL deficiency (<50 ng/ml). As expected, the MBL concentrations are reduced in homozygote variants compared with wild type (Figure 1.) Due to this expected reduction and as no difference was observed in antibody concentrations, MBL concentration was not measured from the infant cohort.

Bottom Line: Follow-up was performed at 3, 5 and 10 years.MBL serum concentration was also measured from the adolescent cohort.No association was found with MBL2 exon 1 polymorphisms and antibody responses against vaccine antigens, after primary and booster dTpa vaccination.

View Article: PubMed Central - PubMed

Affiliation: Department of Infectious Disease Surveillance and Control, National Institute for Health and Welfare (THL), Turku, Finland.

ABSTRACT

Background: Mannose-binding lectin (MBL) is one of the key molecules in innate immunity and its role in human vaccine responses is poorly known. This study aimed to investigate the possible association of MBL polymorphisms with antibody production after primary and booster vaccinations with acellular pertussis vaccines in infants and adolescents.

Methodology/principal findings: Five hundred and sixty eight subjects were included in this study. In the adolescent cohort 355 subjects received a dose of diphtheria and tetanus toxoids and acellular pertussis (dTpa) vaccine ten years previously. Follow-up was performed at 3, 5 and 10 years. Infant cohort consisted of 213 subjects, who had received three primary doses of DTaP vaccine at 3, 5, and 12 months of age according to Finnish immunization program. Blood samples were collected before the vaccinations at 2,5 months of age and after the vaccinations at 13 months and 2 years of age. Concentrations of IgG antibodies to pertussis toxin, filamentous hemagglutinin, and pertactin and antibodies to diphtheria and tetanus toxoids were measured by standardized enzyme-linked immunosorbant assay. Single nucleotide polymorphisms of MBL2 gene exon1 (codons 52, 54, 57) were examined. MBL serum concentration was also measured from the adolescent cohort. No association was found with MBL2 exon 1 polymorphisms and antibody responses against vaccine antigens, after primary and booster dTpa vaccination.

Conclusions: This study indicates that MBL polymorphisms do not affect the production and persistence of antibodies after acellular pertussis vaccination. Our finding also suggests that MBL might not be involved in modulating antibody responses to the vaccines made of purified bacterial proteins.

Show MeSH
Related in: MedlinePlus