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Administration of reconstituted polyphenol oil bodies efficiently suppresses dendritic cell inflammatory pathways and acute intestinal inflammation.

Cavalcanti E, Vadrucci E, Delvecchio FR, Addabbo F, Bettini S, Liou R, Monsurrò V, Huang AY, Pizarro TT, Santino A, Chieppa M - PLoS ONE (2014)

Bottom Line: The in vitro suppressive properties of several polyphenols were first tested and compared for dendritic cells (DCs) production of inflammatory cytokines.Our results showed that administration of low dose reconstituted polyphenol OBs inhibited LPS-mediated inflammatory cytokine secretion, including IL-6, IL-23, and IL-12, while increasing IL-10 and IL-1Rα production.Mice treated with the polyphenol-containing reconstituted OBs (ROBs) were partially protected from dextran sodium sulfate (DSS)-induced colitis and associated weight loss, while mortality and inflammatory scores revealed an overall anti-inflammatory effect that was likely mediated by impaired DC immune responses.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Experimental Immunopathology, IRCCS "de Bellis," Castellana Grotte (BA), Italy.

ABSTRACT
Polyphenols are natural compounds capable of interfering with the inflammatory pathways of several in vitro model systems. In this study, we developed a stable and effective strategy to administer polyphenols to treat in vivo models of acute intestinal inflammation. The in vitro suppressive properties of several polyphenols were first tested and compared for dendritic cells (DCs) production of inflammatory cytokines. A combination of the polyphenols, quercetin and piperine, were then encapsulated into reconstituted oil bodies (OBs) in order to increase their stability. Our results showed that administration of low dose reconstituted polyphenol OBs inhibited LPS-mediated inflammatory cytokine secretion, including IL-6, IL-23, and IL-12, while increasing IL-10 and IL-1Rα production. Mice treated with the polyphenol-containing reconstituted OBs (ROBs) were partially protected from dextran sodium sulfate (DSS)-induced colitis and associated weight loss, while mortality and inflammatory scores revealed an overall anti-inflammatory effect that was likely mediated by impaired DC immune responses. Our study indicates that the administration of reconstituted quercetin and piperine-containing OBs may represent an effective and potent anti-inflammatory strategy to treat acute intestinal inflammation.

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Related in: MedlinePlus

Polyphenol administration to BMDCs interferes with LPS-mediated TNFα and IL-6 production.25 µM of each polyphenol was administered on day 5 and 7; LPS [1 µg/ml] was administered on day 8. SNs were collected 24 h later, and TNFα and IL-6 protein levels were assessed by ELISA. Both cytokine concentrations were significantly inhibited when cells were exposed to Quercetin (Querc) and Piperine (Pip) when compared to control (RPMI+LPS). Data are shown as mean ± S.D. of five independent experiments. Differences where considered between control (RPMI-LPS) and LPS-Quer- or LPS-Pip-treated cells **P<0.01, ***P<0.001. Capsaicin (Caps), Resveratrol (Resv), Rosmarinic Acid (RA), Epigallocatechin (Epig) and Curcumin (Curc).
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pone-0088898-g001: Polyphenol administration to BMDCs interferes with LPS-mediated TNFα and IL-6 production.25 µM of each polyphenol was administered on day 5 and 7; LPS [1 µg/ml] was administered on day 8. SNs were collected 24 h later, and TNFα and IL-6 protein levels were assessed by ELISA. Both cytokine concentrations were significantly inhibited when cells were exposed to Quercetin (Querc) and Piperine (Pip) when compared to control (RPMI+LPS). Data are shown as mean ± S.D. of five independent experiments. Differences where considered between control (RPMI-LPS) and LPS-Quer- or LPS-Pip-treated cells **P<0.01, ***P<0.001. Capsaicin (Caps), Resveratrol (Resv), Rosmarinic Acid (RA), Epigallocatechin (Epig) and Curcumin (Curc).

Mentions: We first compared a battery of synthetic and commercially available polyphenols for their ability to inhibit LPS-induced inflammatory cytokine production from DCs. Bone marrow-derived DCs (BMDCs) received 25 µM of a single polyphenol on the third and fifth day of in vitro culture. On day 7, DCs were exposed to LPS [1 µg/ml], and 24 hours later, supernatants (SNs) were collected, and IL-6 and TNFα protein levels were determined by ELISA (Fig. 1). Quercetin was consistently the most efficient inhibitor of both TNFα and IL-6 protein production compared to other polyphenols, while piperine appeared to have specificity for IL-6 inhibition. Surprisingly, trans-resveratrol, one of the most studied polyphenols, proved to be one of the least efficient inhibitors (Fig. 1).


Administration of reconstituted polyphenol oil bodies efficiently suppresses dendritic cell inflammatory pathways and acute intestinal inflammation.

Cavalcanti E, Vadrucci E, Delvecchio FR, Addabbo F, Bettini S, Liou R, Monsurrò V, Huang AY, Pizarro TT, Santino A, Chieppa M - PLoS ONE (2014)

Polyphenol administration to BMDCs interferes with LPS-mediated TNFα and IL-6 production.25 µM of each polyphenol was administered on day 5 and 7; LPS [1 µg/ml] was administered on day 8. SNs were collected 24 h later, and TNFα and IL-6 protein levels were assessed by ELISA. Both cytokine concentrations were significantly inhibited when cells were exposed to Quercetin (Querc) and Piperine (Pip) when compared to control (RPMI+LPS). Data are shown as mean ± S.D. of five independent experiments. Differences where considered between control (RPMI-LPS) and LPS-Quer- or LPS-Pip-treated cells **P<0.01, ***P<0.001. Capsaicin (Caps), Resveratrol (Resv), Rosmarinic Acid (RA), Epigallocatechin (Epig) and Curcumin (Curc).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3928302&req=5

pone-0088898-g001: Polyphenol administration to BMDCs interferes with LPS-mediated TNFα and IL-6 production.25 µM of each polyphenol was administered on day 5 and 7; LPS [1 µg/ml] was administered on day 8. SNs were collected 24 h later, and TNFα and IL-6 protein levels were assessed by ELISA. Both cytokine concentrations were significantly inhibited when cells were exposed to Quercetin (Querc) and Piperine (Pip) when compared to control (RPMI+LPS). Data are shown as mean ± S.D. of five independent experiments. Differences where considered between control (RPMI-LPS) and LPS-Quer- or LPS-Pip-treated cells **P<0.01, ***P<0.001. Capsaicin (Caps), Resveratrol (Resv), Rosmarinic Acid (RA), Epigallocatechin (Epig) and Curcumin (Curc).
Mentions: We first compared a battery of synthetic and commercially available polyphenols for their ability to inhibit LPS-induced inflammatory cytokine production from DCs. Bone marrow-derived DCs (BMDCs) received 25 µM of a single polyphenol on the third and fifth day of in vitro culture. On day 7, DCs were exposed to LPS [1 µg/ml], and 24 hours later, supernatants (SNs) were collected, and IL-6 and TNFα protein levels were determined by ELISA (Fig. 1). Quercetin was consistently the most efficient inhibitor of both TNFα and IL-6 protein production compared to other polyphenols, while piperine appeared to have specificity for IL-6 inhibition. Surprisingly, trans-resveratrol, one of the most studied polyphenols, proved to be one of the least efficient inhibitors (Fig. 1).

Bottom Line: The in vitro suppressive properties of several polyphenols were first tested and compared for dendritic cells (DCs) production of inflammatory cytokines.Our results showed that administration of low dose reconstituted polyphenol OBs inhibited LPS-mediated inflammatory cytokine secretion, including IL-6, IL-23, and IL-12, while increasing IL-10 and IL-1Rα production.Mice treated with the polyphenol-containing reconstituted OBs (ROBs) were partially protected from dextran sodium sulfate (DSS)-induced colitis and associated weight loss, while mortality and inflammatory scores revealed an overall anti-inflammatory effect that was likely mediated by impaired DC immune responses.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Experimental Immunopathology, IRCCS "de Bellis," Castellana Grotte (BA), Italy.

ABSTRACT
Polyphenols are natural compounds capable of interfering with the inflammatory pathways of several in vitro model systems. In this study, we developed a stable and effective strategy to administer polyphenols to treat in vivo models of acute intestinal inflammation. The in vitro suppressive properties of several polyphenols were first tested and compared for dendritic cells (DCs) production of inflammatory cytokines. A combination of the polyphenols, quercetin and piperine, were then encapsulated into reconstituted oil bodies (OBs) in order to increase their stability. Our results showed that administration of low dose reconstituted polyphenol OBs inhibited LPS-mediated inflammatory cytokine secretion, including IL-6, IL-23, and IL-12, while increasing IL-10 and IL-1Rα production. Mice treated with the polyphenol-containing reconstituted OBs (ROBs) were partially protected from dextran sodium sulfate (DSS)-induced colitis and associated weight loss, while mortality and inflammatory scores revealed an overall anti-inflammatory effect that was likely mediated by impaired DC immune responses. Our study indicates that the administration of reconstituted quercetin and piperine-containing OBs may represent an effective and potent anti-inflammatory strategy to treat acute intestinal inflammation.

Show MeSH
Related in: MedlinePlus