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Transcriptome profile of Trichoderma harzianum IOC-3844 induced by sugarcane bagasse.

Horta MA, Vicentini R, Delabona Pda S, Laborda P, Crucello A, Freitas S, Kuroshu RM, Polikarpov I, Pradella JG, Souza AP - PLoS ONE (2014)

Bottom Line: De novo assembly resulted in 32,396 contigs, which were submitted for identification and classified according to their identities.This analysis allowed us to define a principal set of T. harzianum genes that are involved in the degradation of cellulose and hemicellulose and the accessory genes that are involved in the depolymerization of biomass.The present study provides valuable information for future studies on biomass degradation and contributes to a better understanding of the role of the genes that are involved in this process.

View Article: PubMed Central - PubMed

Affiliation: Center for Molecular Biology and Genetic Engineering (CBMEG), University of Campinas (UNICAMP), Campinas, SP, Brazil.

ABSTRACT
Profiling the transcriptome that underlies biomass degradation by the fungus Trichoderma harzianum allows the identification of gene sequences with potential application in enzymatic hydrolysis processing. In the present study, the transcriptome of T. harzianum IOC-3844 was analyzed using RNA-seq technology. The sequencing generated 14.7 Gbp for downstream analyses. De novo assembly resulted in 32,396 contigs, which were submitted for identification and classified according to their identities. This analysis allowed us to define a principal set of T. harzianum genes that are involved in the degradation of cellulose and hemicellulose and the accessory genes that are involved in the depolymerization of biomass. An additional analysis of expression levels identified a set of carbohydrate-active enzymes that are upregulated under different conditions. The present study provides valuable information for future studies on biomass degradation and contributes to a better understanding of the role of the genes that are involved in this process.

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Related in: MedlinePlus

Filter paper activity enzymatic profiles (FPU mL−1 h−1).T. harzianum fermentation on a complex substrate (DSB) from extracts grown on preculture media using DSB (▵), cellulose (◊) or lactose (□) as the carbon source. The differences were considered significant at P<0.05 (Student's t-test) and are indicated by (a) for cellulose, (b) for DSB and (c) for lactose samples.
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pone-0088689-g002: Filter paper activity enzymatic profiles (FPU mL−1 h−1).T. harzianum fermentation on a complex substrate (DSB) from extracts grown on preculture media using DSB (▵), cellulose (◊) or lactose (□) as the carbon source. The differences were considered significant at P<0.05 (Student's t-test) and are indicated by (a) for cellulose, (b) for DSB and (c) for lactose samples.

Mentions: The FPase was evaluated to determine the enzymatic activity profile of the cellulases during 129 h of fermentation (Figure 2) using DSB as a carbon source. RNA was isolated from the mycelia at 96 h of cultivation; this time point was associated with a significant production of FPase (0.53 FPU mL−1) (Figure 2). This 96 h cultivation period included a 48 h adaptation phase. Previous studies have indicated that the enzymatic activity of this fungus, as measured by cellulase production, is lower when grown on soluble carbon sources than when grown on DSB, and these results are in agreement with the present study [4]. The substrate acts as both an adhesion surface and as fermentable biomass for the fungi, and it activates the synthesis of hydrolytic complexes.


Transcriptome profile of Trichoderma harzianum IOC-3844 induced by sugarcane bagasse.

Horta MA, Vicentini R, Delabona Pda S, Laborda P, Crucello A, Freitas S, Kuroshu RM, Polikarpov I, Pradella JG, Souza AP - PLoS ONE (2014)

Filter paper activity enzymatic profiles (FPU mL−1 h−1).T. harzianum fermentation on a complex substrate (DSB) from extracts grown on preculture media using DSB (▵), cellulose (◊) or lactose (□) as the carbon source. The differences were considered significant at P<0.05 (Student's t-test) and are indicated by (a) for cellulose, (b) for DSB and (c) for lactose samples.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3928278&req=5

pone-0088689-g002: Filter paper activity enzymatic profiles (FPU mL−1 h−1).T. harzianum fermentation on a complex substrate (DSB) from extracts grown on preculture media using DSB (▵), cellulose (◊) or lactose (□) as the carbon source. The differences were considered significant at P<0.05 (Student's t-test) and are indicated by (a) for cellulose, (b) for DSB and (c) for lactose samples.
Mentions: The FPase was evaluated to determine the enzymatic activity profile of the cellulases during 129 h of fermentation (Figure 2) using DSB as a carbon source. RNA was isolated from the mycelia at 96 h of cultivation; this time point was associated with a significant production of FPase (0.53 FPU mL−1) (Figure 2). This 96 h cultivation period included a 48 h adaptation phase. Previous studies have indicated that the enzymatic activity of this fungus, as measured by cellulase production, is lower when grown on soluble carbon sources than when grown on DSB, and these results are in agreement with the present study [4]. The substrate acts as both an adhesion surface and as fermentable biomass for the fungi, and it activates the synthesis of hydrolytic complexes.

Bottom Line: De novo assembly resulted in 32,396 contigs, which were submitted for identification and classified according to their identities.This analysis allowed us to define a principal set of T. harzianum genes that are involved in the degradation of cellulose and hemicellulose and the accessory genes that are involved in the depolymerization of biomass.The present study provides valuable information for future studies on biomass degradation and contributes to a better understanding of the role of the genes that are involved in this process.

View Article: PubMed Central - PubMed

Affiliation: Center for Molecular Biology and Genetic Engineering (CBMEG), University of Campinas (UNICAMP), Campinas, SP, Brazil.

ABSTRACT
Profiling the transcriptome that underlies biomass degradation by the fungus Trichoderma harzianum allows the identification of gene sequences with potential application in enzymatic hydrolysis processing. In the present study, the transcriptome of T. harzianum IOC-3844 was analyzed using RNA-seq technology. The sequencing generated 14.7 Gbp for downstream analyses. De novo assembly resulted in 32,396 contigs, which were submitted for identification and classified according to their identities. This analysis allowed us to define a principal set of T. harzianum genes that are involved in the degradation of cellulose and hemicellulose and the accessory genes that are involved in the depolymerization of biomass. An additional analysis of expression levels identified a set of carbohydrate-active enzymes that are upregulated under different conditions. The present study provides valuable information for future studies on biomass degradation and contributes to a better understanding of the role of the genes that are involved in this process.

Show MeSH
Related in: MedlinePlus