Limits...
Proteomic analysis of hepatic tissue of Cyprinus carpio L. exposed to cyanobacterial blooms in Lake Taihu, China.

Jiang J, Wang X, Shan Z, Yang L, Zhou J, Bu Y - PLoS ONE (2014)

Bottom Line: MCs were transported into liver cells and induced the excessive production of reactive oxygen species (ROS).MCs and ROS inhibited protein phosphatase and aldehyde dehydrogenase (ALDH), directly or indirectly resulting in oxidative stress and disruption of the cytoskeleton.These effects further interfered with metabolic pathways in the liver through the regulation of series of related proteins.

View Article: PubMed Central - PubMed

Affiliation: Nanjing Institute of Environmental Sciences/Key Laboratory of Pesticide Environmental Assessment and Pollution Control, Ministry of Environmental Protection, Nanjing, People's Republic of China.

ABSTRACT
With the rapid development of industry and agriculture and associated pollution, the cyanobacterial blooms in Lake Taihu have become a major threat to aquatic wildlife and human health. In this study, the ecotoxicological effects of cyanobacterial blooms on cage-cultured carp (Cyprinus carpio L.) in Meiliang Bay of Lake Taihu were investigated. Microcystins (MCs), major cyanobacterial toxins, have been detected in carp cultured at different experimental sites of Meiliang Bay. We observed that the accumulation of MCs in carp was closely associated with several environmental factors, including temperature, pH value, and density of cyanobacterial blooms. The proteomic profile of carp liver exposed to cyanobacterial blooms was analyzed using two-dimensional difference in-gel electrophoresis (2D-DIGE) and mass spectrometry. The toxic effects of cyanobacterial blooms on carp liver were similar to changes caused by MCs. MCs were transported into liver cells and induced the excessive production of reactive oxygen species (ROS). MCs and ROS inhibited protein phosphatase and aldehyde dehydrogenase (ALDH), directly or indirectly resulting in oxidative stress and disruption of the cytoskeleton. These effects further interfered with metabolic pathways in the liver through the regulation of series of related proteins. The results of this study indicated that cyanobacterial blooms pose a major threat to aquatic wildlife in Meiliang Bay in Lake Taihu. These results provided evidence of the molecular mechanisms underlying liver damage in carp exposed to cyanobacterial blooms.

Show MeSH

Related in: MedlinePlus

Functional classification of up-regulated (A)/down-regulated (B) proteins from the liver of C. carpio in the T2 group.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3928196&req=5

pone-0088211-g006: Functional classification of up-regulated (A)/down-regulated (B) proteins from the liver of C. carpio in the T2 group.

Mentions: The biological functions of the proteins were analyzed based on the GenBank, Gene Ontology and KEGG databases to reveal the potential toxic mechanism underlying cyanobacterial blooms (Table 2). The classification and up- and down regulation of these proteins are shown in Figure 5. Among the 57 identified proteins, 25 proteins (43.9%) were involved in metabolic processes. Among these, 10 proteins were involved in amino acid metabolism, 6 proteins were involved in glucose metabolism and 4 proteins were associated with lipid metabolism. In addition, 9 proteins were associated with the cytoskeleton reorganization and stress. Among 57 proteins identified, 23 proteins were up-regulated and 19 proteins were down-regulated among the carp cultured at different experiment sites. Changes greater than 1.5-fold were observed in 45 of the 57 proteins identified. Compared with the control and T1 groups, a significant difference in the protein expression was observed in the T2 group. The functional classification of significantly up/down-regulated proteins from the liver of C. carpio in the T2 group is shown in Figure 6. A total of 31 up-regulated proteins were involved in metabolic processes, cytoskeleton reorganization, and stress defense, while 26 down-regulated proteins were involved in metabolic processes, stress defense, protein translation and protein modifications.


Proteomic analysis of hepatic tissue of Cyprinus carpio L. exposed to cyanobacterial blooms in Lake Taihu, China.

Jiang J, Wang X, Shan Z, Yang L, Zhou J, Bu Y - PLoS ONE (2014)

Functional classification of up-regulated (A)/down-regulated (B) proteins from the liver of C. carpio in the T2 group.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3928196&req=5

pone-0088211-g006: Functional classification of up-regulated (A)/down-regulated (B) proteins from the liver of C. carpio in the T2 group.
Mentions: The biological functions of the proteins were analyzed based on the GenBank, Gene Ontology and KEGG databases to reveal the potential toxic mechanism underlying cyanobacterial blooms (Table 2). The classification and up- and down regulation of these proteins are shown in Figure 5. Among the 57 identified proteins, 25 proteins (43.9%) were involved in metabolic processes. Among these, 10 proteins were involved in amino acid metabolism, 6 proteins were involved in glucose metabolism and 4 proteins were associated with lipid metabolism. In addition, 9 proteins were associated with the cytoskeleton reorganization and stress. Among 57 proteins identified, 23 proteins were up-regulated and 19 proteins were down-regulated among the carp cultured at different experiment sites. Changes greater than 1.5-fold were observed in 45 of the 57 proteins identified. Compared with the control and T1 groups, a significant difference in the protein expression was observed in the T2 group. The functional classification of significantly up/down-regulated proteins from the liver of C. carpio in the T2 group is shown in Figure 6. A total of 31 up-regulated proteins were involved in metabolic processes, cytoskeleton reorganization, and stress defense, while 26 down-regulated proteins were involved in metabolic processes, stress defense, protein translation and protein modifications.

Bottom Line: MCs were transported into liver cells and induced the excessive production of reactive oxygen species (ROS).MCs and ROS inhibited protein phosphatase and aldehyde dehydrogenase (ALDH), directly or indirectly resulting in oxidative stress and disruption of the cytoskeleton.These effects further interfered with metabolic pathways in the liver through the regulation of series of related proteins.

View Article: PubMed Central - PubMed

Affiliation: Nanjing Institute of Environmental Sciences/Key Laboratory of Pesticide Environmental Assessment and Pollution Control, Ministry of Environmental Protection, Nanjing, People's Republic of China.

ABSTRACT
With the rapid development of industry and agriculture and associated pollution, the cyanobacterial blooms in Lake Taihu have become a major threat to aquatic wildlife and human health. In this study, the ecotoxicological effects of cyanobacterial blooms on cage-cultured carp (Cyprinus carpio L.) in Meiliang Bay of Lake Taihu were investigated. Microcystins (MCs), major cyanobacterial toxins, have been detected in carp cultured at different experimental sites of Meiliang Bay. We observed that the accumulation of MCs in carp was closely associated with several environmental factors, including temperature, pH value, and density of cyanobacterial blooms. The proteomic profile of carp liver exposed to cyanobacterial blooms was analyzed using two-dimensional difference in-gel electrophoresis (2D-DIGE) and mass spectrometry. The toxic effects of cyanobacterial blooms on carp liver were similar to changes caused by MCs. MCs were transported into liver cells and induced the excessive production of reactive oxygen species (ROS). MCs and ROS inhibited protein phosphatase and aldehyde dehydrogenase (ALDH), directly or indirectly resulting in oxidative stress and disruption of the cytoskeleton. These effects further interfered with metabolic pathways in the liver through the regulation of series of related proteins. The results of this study indicated that cyanobacterial blooms pose a major threat to aquatic wildlife in Meiliang Bay in Lake Taihu. These results provided evidence of the molecular mechanisms underlying liver damage in carp exposed to cyanobacterial blooms.

Show MeSH
Related in: MedlinePlus