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A novel in vivo platform for studying alveolar bone regeneration in rat.

Kim JH, Moon HJ, Kim TH, Jo JM, Yang SH, Naskar D, Kundu SC, Chrzanowski W, Kim HW - J Tissue Eng (2013)

Bottom Line: Rat premaxillary bone defects were filled with silk scaffold or remained empty during the implantation period (up to 6 weeks), and harvested samples were analyzed by micro-computed tomography and histopathology.Empty defects showed increased but limited new bone formation with increasing implantation period.In defects implanted with silk sponge, the bone formation was significantly greater than that of empty defect, indicating an effective role of silk scaffold in the defect model.

View Article: PubMed Central - PubMed

Affiliation: Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan, Republic of Korea ; Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan, Republic of Korea.

ABSTRACT
Alveolar bone regeneration is a significant challenge in dental implantation. Novel biomaterials and tissue-engineered constructs are under extensive development and awaiting in vivo animal tests to find clinical endpoint. Here, we establish a novel in vivo model, modifying gingivoperiosteoplasty in rat for the alveolar bone regeneration. Rat premaxillary bone defects were filled with silk scaffold or remained empty during the implantation period (up to 6 weeks), and harvested samples were analyzed by micro-computed tomography and histopathology. Empty defects showed increased but limited new bone formation with increasing implantation period. In defects implanted with silk sponge, the bone formation was significantly greater than that of empty defect, indicating an effective role of silk scaffold in the defect model. The modified premaxillary defect model in rat is simple to perform, while mimicking the clinical conditions, finding usefulness for the development of biomaterials and tissue-engineered constructs targeting alveolar bone regeneration in dental implantation.

No MeSH data available.


Related in: MedlinePlus

(a–d) 2D and (e–h) 3D micro-computed tomographic images, displaying new bone and defect area on (a and e) 2 weeks, (b and f) 4 weeks, and (c and g) 6 weeks of defect group and (d and h) 6 weeks of silk-implanted group. Note the increased bone formation in defects over time, and the improved growth at 6 weeks for the silk-implanted group. (a–d—white dot line: defect margin, white arrow: bone growing direction; e–h—red arrow: defect margin).
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fig4-2041731413517705: (a–d) 2D and (e–h) 3D micro-computed tomographic images, displaying new bone and defect area on (a and e) 2 weeks, (b and f) 4 weeks, and (c and g) 6 weeks of defect group and (d and h) 6 weeks of silk-implanted group. Note the increased bone formation in defects over time, and the improved growth at 6 weeks for the silk-implanted group. (a–d—white dot line: defect margin, white arrow: bone growing direction; e–h—red arrow: defect margin).

Mentions: Samples, including scaffolds and surrounding tissues, were imaged and analyzed using high-resolution µ-CT, and 2D and 3D images were constructed for four groups (Figure 4). µ-CT examination provided evidence that only minimal bone healing occurred in the empty defect group at 2 weeks post operation. However, bone formation increased in the empty defect group over time. For the silk scaffold group, substantial bone formation was evidenced, which was noticeably higher than that of the empty defect group at 6 weeks. Based on the images, a level of hard tissue formation occurred from the defect margin to the central region in the empty defect but the defect recovery was substantially limited, which, however, considerably improved when the scaffold was implanted.


A novel in vivo platform for studying alveolar bone regeneration in rat.

Kim JH, Moon HJ, Kim TH, Jo JM, Yang SH, Naskar D, Kundu SC, Chrzanowski W, Kim HW - J Tissue Eng (2013)

(a–d) 2D and (e–h) 3D micro-computed tomographic images, displaying new bone and defect area on (a and e) 2 weeks, (b and f) 4 weeks, and (c and g) 6 weeks of defect group and (d and h) 6 weeks of silk-implanted group. Note the increased bone formation in defects over time, and the improved growth at 6 weeks for the silk-implanted group. (a–d—white dot line: defect margin, white arrow: bone growing direction; e–h—red arrow: defect margin).
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2 - License 3
Show All Figures
getmorefigures.php?uid=PMC3927964&req=5

fig4-2041731413517705: (a–d) 2D and (e–h) 3D micro-computed tomographic images, displaying new bone and defect area on (a and e) 2 weeks, (b and f) 4 weeks, and (c and g) 6 weeks of defect group and (d and h) 6 weeks of silk-implanted group. Note the increased bone formation in defects over time, and the improved growth at 6 weeks for the silk-implanted group. (a–d—white dot line: defect margin, white arrow: bone growing direction; e–h—red arrow: defect margin).
Mentions: Samples, including scaffolds and surrounding tissues, were imaged and analyzed using high-resolution µ-CT, and 2D and 3D images were constructed for four groups (Figure 4). µ-CT examination provided evidence that only minimal bone healing occurred in the empty defect group at 2 weeks post operation. However, bone formation increased in the empty defect group over time. For the silk scaffold group, substantial bone formation was evidenced, which was noticeably higher than that of the empty defect group at 6 weeks. Based on the images, a level of hard tissue formation occurred from the defect margin to the central region in the empty defect but the defect recovery was substantially limited, which, however, considerably improved when the scaffold was implanted.

Bottom Line: Rat premaxillary bone defects were filled with silk scaffold or remained empty during the implantation period (up to 6 weeks), and harvested samples were analyzed by micro-computed tomography and histopathology.Empty defects showed increased but limited new bone formation with increasing implantation period.In defects implanted with silk sponge, the bone formation was significantly greater than that of empty defect, indicating an effective role of silk scaffold in the defect model.

View Article: PubMed Central - PubMed

Affiliation: Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan, Republic of Korea ; Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan, Republic of Korea.

ABSTRACT
Alveolar bone regeneration is a significant challenge in dental implantation. Novel biomaterials and tissue-engineered constructs are under extensive development and awaiting in vivo animal tests to find clinical endpoint. Here, we establish a novel in vivo model, modifying gingivoperiosteoplasty in rat for the alveolar bone regeneration. Rat premaxillary bone defects were filled with silk scaffold or remained empty during the implantation period (up to 6 weeks), and harvested samples were analyzed by micro-computed tomography and histopathology. Empty defects showed increased but limited new bone formation with increasing implantation period. In defects implanted with silk sponge, the bone formation was significantly greater than that of empty defect, indicating an effective role of silk scaffold in the defect model. The modified premaxillary defect model in rat is simple to perform, while mimicking the clinical conditions, finding usefulness for the development of biomaterials and tissue-engineered constructs targeting alveolar bone regeneration in dental implantation.

No MeSH data available.


Related in: MedlinePlus