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A novel in vivo platform for studying alveolar bone regeneration in rat.

Kim JH, Moon HJ, Kim TH, Jo JM, Yang SH, Naskar D, Kundu SC, Chrzanowski W, Kim HW - J Tissue Eng (2013)

Bottom Line: Rat premaxillary bone defects were filled with silk scaffold or remained empty during the implantation period (up to 6 weeks), and harvested samples were analyzed by micro-computed tomography and histopathology.Empty defects showed increased but limited new bone formation with increasing implantation period.In defects implanted with silk sponge, the bone formation was significantly greater than that of empty defect, indicating an effective role of silk scaffold in the defect model.

View Article: PubMed Central - PubMed

Affiliation: Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan, Republic of Korea ; Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan, Republic of Korea.

ABSTRACT
Alveolar bone regeneration is a significant challenge in dental implantation. Novel biomaterials and tissue-engineered constructs are under extensive development and awaiting in vivo animal tests to find clinical endpoint. Here, we establish a novel in vivo model, modifying gingivoperiosteoplasty in rat for the alveolar bone regeneration. Rat premaxillary bone defects were filled with silk scaffold or remained empty during the implantation period (up to 6 weeks), and harvested samples were analyzed by micro-computed tomography and histopathology. Empty defects showed increased but limited new bone formation with increasing implantation period. In defects implanted with silk sponge, the bone formation was significantly greater than that of empty defect, indicating an effective role of silk scaffold in the defect model. The modified premaxillary defect model in rat is simple to perform, while mimicking the clinical conditions, finding usefulness for the development of biomaterials and tissue-engineered constructs targeting alveolar bone regeneration in dental implantation.

No MeSH data available.


Related in: MedlinePlus

Fabrication method of 3D sponges from nonmulberry silk protein fibroin.3D: three-dimensional; SDS: sodium dodecyl sulfate.
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fig1-2041731413517705: Fabrication method of 3D sponges from nonmulberry silk protein fibroin.3D: three-dimensional; SDS: sodium dodecyl sulfate.

Mentions: The silk scaffolds were fabricated according to the method depicted in the Figure 1. In brief, crude silk protein fibroin was first isolated from the silkglands of nonmulberry (Antheraea mylitta) mature fifth instar larva, grown in the farm of Indian Institute of Technology, Kharagpur, according to the protocol described elsewhere.19 The isolated protein was dissolved in 1% sodium dodecyl sulfate (SDS) containing 10 mM Tris (pH 8.0) and 5 mM ethylenediaminetetraacetic acid (EDTA). The solution was then taken in a 12-kDa cellulose dialysis membrane and dialyzed against deionized water for 8 h with frequent water change.


A novel in vivo platform for studying alveolar bone regeneration in rat.

Kim JH, Moon HJ, Kim TH, Jo JM, Yang SH, Naskar D, Kundu SC, Chrzanowski W, Kim HW - J Tissue Eng (2013)

Fabrication method of 3D sponges from nonmulberry silk protein fibroin.3D: three-dimensional; SDS: sodium dodecyl sulfate.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2 - License 3
Show All Figures
getmorefigures.php?uid=PMC3927964&req=5

fig1-2041731413517705: Fabrication method of 3D sponges from nonmulberry silk protein fibroin.3D: three-dimensional; SDS: sodium dodecyl sulfate.
Mentions: The silk scaffolds were fabricated according to the method depicted in the Figure 1. In brief, crude silk protein fibroin was first isolated from the silkglands of nonmulberry (Antheraea mylitta) mature fifth instar larva, grown in the farm of Indian Institute of Technology, Kharagpur, according to the protocol described elsewhere.19 The isolated protein was dissolved in 1% sodium dodecyl sulfate (SDS) containing 10 mM Tris (pH 8.0) and 5 mM ethylenediaminetetraacetic acid (EDTA). The solution was then taken in a 12-kDa cellulose dialysis membrane and dialyzed against deionized water for 8 h with frequent water change.

Bottom Line: Rat premaxillary bone defects were filled with silk scaffold or remained empty during the implantation period (up to 6 weeks), and harvested samples were analyzed by micro-computed tomography and histopathology.Empty defects showed increased but limited new bone formation with increasing implantation period.In defects implanted with silk sponge, the bone formation was significantly greater than that of empty defect, indicating an effective role of silk scaffold in the defect model.

View Article: PubMed Central - PubMed

Affiliation: Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan, Republic of Korea ; Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan, Republic of Korea.

ABSTRACT
Alveolar bone regeneration is a significant challenge in dental implantation. Novel biomaterials and tissue-engineered constructs are under extensive development and awaiting in vivo animal tests to find clinical endpoint. Here, we establish a novel in vivo model, modifying gingivoperiosteoplasty in rat for the alveolar bone regeneration. Rat premaxillary bone defects were filled with silk scaffold or remained empty during the implantation period (up to 6 weeks), and harvested samples were analyzed by micro-computed tomography and histopathology. Empty defects showed increased but limited new bone formation with increasing implantation period. In defects implanted with silk sponge, the bone formation was significantly greater than that of empty defect, indicating an effective role of silk scaffold in the defect model. The modified premaxillary defect model in rat is simple to perform, while mimicking the clinical conditions, finding usefulness for the development of biomaterials and tissue-engineered constructs targeting alveolar bone regeneration in dental implantation.

No MeSH data available.


Related in: MedlinePlus