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Cloning and characterization of a flavonol synthase gene from Scutellaria baicalensis.

Kim YB, Kim K, Kim Y, Tuan PA, Kim HH, Cho JW, Park SU - ScientificWorldJournal (2014)

Bottom Line: The myricetin content was significantly higher than that of kaempferol and quercetin.Therefore, we suggest that SbFLS mediates flavonol formation in the different organs of S. baicalensis.Our study may contribute to the knowledge of the role of FLS in S. baicalensis.

View Article: PubMed Central - PubMed

Affiliation: Department of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 305-764, Republic of Korea.

ABSTRACT
Flavonols are the most abundant of all the flavonoids and play pivotal roles in a variety of plants. We isolated a cDNA clone encoding flavonol synthase from Scutellaria baicalensis (SbFLS). The SbFLS cDNA is 1011 bp long, encodes 336 amino acid residues, and belongs to a family of 2-oxoglutarate-dependent dioxygenases. The overall structure of SbFLS is very similar to that of Arabidopsis thaliana anthocyanidin synthase (AtANS), with a β jelly-roll fold surrounded by tens of short and long α-helices. SbFLS was constitutively expressed in the roots, stems, leaves, and flowers, with particularly high expression in the roots and flowers. SbFLS transcript levels in the roots were 376-, 70-, and 2.5-fold higher than in the leaves, stems, and flowers. The myricetin content was significantly higher than that of kaempferol and quercetin. Therefore, we suggest that SbFLS mediates flavonol formation in the different organs of S. baicalensis. Our study may contribute to the knowledge of the role of FLS in S. baicalensis.

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Related in: MedlinePlus

Flavonol content in different organs of S. baicalensis. The height of each bar and error bars represent the means and standard errors, respectively, from 3 independent measurements.
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fig5: Flavonol content in different organs of S. baicalensis. The height of each bar and error bars represent the means and standard errors, respectively, from 3 independent measurements.

Mentions: Kaempferol, quercetin, and myricetin were detected in almost all tissues (i.e., flowers, stems, leaves, and roots) by HPLC (Figure 5). The myricetin content was significantly higher than that of kaempferol and quercetin. The transcription pattern of SbFLS was similar to the accumulation pattern of kaempferol. Roots showed the highest kaempferol content (0.97 mg g−1 dry weight (DW)); the leaves and flowers contained similar amounts (0.62 and 0.51 mg g−1 DW), but no kaempferol was detected in the stem. Kaempferol and some glycoside derivatives have a wide range of pharmacological activities [29] and account for the utility of dry Scutellaria root as a multipurpose herb in oriental medicine. The leaves contained the highest levels of quercetin (1.26 mg g−1 DW), whereas the lowest amount of quercetin was in the flowers (0.64 mg g−1 DW). Interestingly, roots displayed the lowest amount of myricetin (0.8 mg g−1 DW), whereas the highest myricetin content was found in the flowers (6.05 mg g−1 DW). The myricetin content in the flowers was 2.2- and 7.5-fold higher than that in the leaves and roots, respectively. Feng and Liu [30] reported that the quercetin content of leaves was significantly higher than the content in pseudostems in different tissues of Welsh onion (Allium fistulosum L.).


Cloning and characterization of a flavonol synthase gene from Scutellaria baicalensis.

Kim YB, Kim K, Kim Y, Tuan PA, Kim HH, Cho JW, Park SU - ScientificWorldJournal (2014)

Flavonol content in different organs of S. baicalensis. The height of each bar and error bars represent the means and standard errors, respectively, from 3 independent measurements.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3927949&req=5

fig5: Flavonol content in different organs of S. baicalensis. The height of each bar and error bars represent the means and standard errors, respectively, from 3 independent measurements.
Mentions: Kaempferol, quercetin, and myricetin were detected in almost all tissues (i.e., flowers, stems, leaves, and roots) by HPLC (Figure 5). The myricetin content was significantly higher than that of kaempferol and quercetin. The transcription pattern of SbFLS was similar to the accumulation pattern of kaempferol. Roots showed the highest kaempferol content (0.97 mg g−1 dry weight (DW)); the leaves and flowers contained similar amounts (0.62 and 0.51 mg g−1 DW), but no kaempferol was detected in the stem. Kaempferol and some glycoside derivatives have a wide range of pharmacological activities [29] and account for the utility of dry Scutellaria root as a multipurpose herb in oriental medicine. The leaves contained the highest levels of quercetin (1.26 mg g−1 DW), whereas the lowest amount of quercetin was in the flowers (0.64 mg g−1 DW). Interestingly, roots displayed the lowest amount of myricetin (0.8 mg g−1 DW), whereas the highest myricetin content was found in the flowers (6.05 mg g−1 DW). The myricetin content in the flowers was 2.2- and 7.5-fold higher than that in the leaves and roots, respectively. Feng and Liu [30] reported that the quercetin content of leaves was significantly higher than the content in pseudostems in different tissues of Welsh onion (Allium fistulosum L.).

Bottom Line: The myricetin content was significantly higher than that of kaempferol and quercetin.Therefore, we suggest that SbFLS mediates flavonol formation in the different organs of S. baicalensis.Our study may contribute to the knowledge of the role of FLS in S. baicalensis.

View Article: PubMed Central - PubMed

Affiliation: Department of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 305-764, Republic of Korea.

ABSTRACT
Flavonols are the most abundant of all the flavonoids and play pivotal roles in a variety of plants. We isolated a cDNA clone encoding flavonol synthase from Scutellaria baicalensis (SbFLS). The SbFLS cDNA is 1011 bp long, encodes 336 amino acid residues, and belongs to a family of 2-oxoglutarate-dependent dioxygenases. The overall structure of SbFLS is very similar to that of Arabidopsis thaliana anthocyanidin synthase (AtANS), with a β jelly-roll fold surrounded by tens of short and long α-helices. SbFLS was constitutively expressed in the roots, stems, leaves, and flowers, with particularly high expression in the roots and flowers. SbFLS transcript levels in the roots were 376-, 70-, and 2.5-fold higher than in the leaves, stems, and flowers. The myricetin content was significantly higher than that of kaempferol and quercetin. Therefore, we suggest that SbFLS mediates flavonol formation in the different organs of S. baicalensis. Our study may contribute to the knowledge of the role of FLS in S. baicalensis.

Show MeSH
Related in: MedlinePlus