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Olive leaf extract attenuates obesity in high-fat diet-fed mice by modulating the expression of molecules involved in adipogenesis and thermogenesis.

Shen Y, Song SJ, Keum N, Park T - Evid Based Complement Alternat Med (2014)

Bottom Line: OLD-fed mice showed significantly reduced body weight gain, visceral fat-pad weights, and plasma lipid levels as compared with HFD-fed mice.OLE significantly reversed the HFD-induced upregulation of WNT10b- and galanin-mediated signaling molecules and key adipogenic genes (PPAR γ , C/EBP α , CD36, FAS, and leptin) in the epididymal adipose tissue of HFD-fed mice.Furthermore, the HFD-induced downregulation of thermogenic genes involved in uncoupled respiration (SIRT1, PGC1 α , and UCP1) and mitochondrial biogenesis (TFAM, NRF-1, and COX2) was also significantly reversed by OLE.

View Article: PubMed Central - PubMed

Affiliation: Department of Food and Nutrition, Brain Korea 21 PLUS Project, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-749, Republic of Korea.

ABSTRACT
The present study aimed to investigate whether olive leaf extract (OLE) prevents high-fat diet (HFD)-induced obesity in mice and to explore the underlying mechanisms. Mice were randomly divided into groups that received a chow diet (CD), HFD, or 0.15% OLE-supplemented diet (OLD) for 8 weeks. OLD-fed mice showed significantly reduced body weight gain, visceral fat-pad weights, and plasma lipid levels as compared with HFD-fed mice. OLE significantly reversed the HFD-induced upregulation of WNT10b- and galanin-mediated signaling molecules and key adipogenic genes (PPAR γ , C/EBP α , CD36, FAS, and leptin) in the epididymal adipose tissue of HFD-fed mice. Furthermore, the HFD-induced downregulation of thermogenic genes involved in uncoupled respiration (SIRT1, PGC1 α , and UCP1) and mitochondrial biogenesis (TFAM, NRF-1, and COX2) was also significantly reversed by OLE. These results suggest that OLE exerts beneficial effects against obesity by regulating the expression of genes involved in adipogenesis and thermogenesis in the visceral adipose tissue of HFD-fed mice.

No MeSH data available.


Related in: MedlinePlus

Effects of OLE on body weight gain, food efficiency ratio (FER), and visceral fat-pad weights of HFD-fed mice. Mice were fed CD, HFD, OLD, or SD for 8 weeks. Changes in the (a) body weight, (b) body weight gain, (c) food intake, (d) FER, and (e) visceral fat-pad weights. (f) Histological analysis of the epididymal adipose tissue from each group was conducted to quantify the size of adipocyte. (Left) Representative pictures of hematoxylin-eosin (H&E)-stained fat cells from the mice epididymal adipose tissue (×100). (Right) Densitometric analysis of the average adipocyte diameter in the epididymal adipose tissue of mice. Values are presented as means ± SEM (n = 8). Mean values indicated with different letters indicate statistical significance (P < 0.05); FER = Body weight gain for experimental period (g)/food intake for experimental period (g).
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fig2: Effects of OLE on body weight gain, food efficiency ratio (FER), and visceral fat-pad weights of HFD-fed mice. Mice were fed CD, HFD, OLD, or SD for 8 weeks. Changes in the (a) body weight, (b) body weight gain, (c) food intake, (d) FER, and (e) visceral fat-pad weights. (f) Histological analysis of the epididymal adipose tissue from each group was conducted to quantify the size of adipocyte. (Left) Representative pictures of hematoxylin-eosin (H&E)-stained fat cells from the mice epididymal adipose tissue (×100). (Right) Densitometric analysis of the average adipocyte diameter in the epididymal adipose tissue of mice. Values are presented as means ± SEM (n = 8). Mean values indicated with different letters indicate statistical significance (P < 0.05); FER = Body weight gain for experimental period (g)/food intake for experimental period (g).

Mentions: HFD-fed mice showed significant increases in final body weight (38%, P < 0.05) and cumulative body weight gain (162%, P < 0.05) as compared with CD-fed mice. OLD-fed mice showed a 37% reduction in final body weight and an 80% decrease in body weight gain as compared with HFD-fed mice (Figures 2(a) and 2(b)). The food intake was also significantly lower in OLD-fed mice (−26%, P < 0.05) than in HFD-fed mice during the 8-week feeding period. The food efficiency ratio (FER) (−74%, P < 0.05) of OLD-fed mice was significantly lower than that of HFD-fed mice (Figures 2(c) and 2(d)). The cumulative caloric intake over 8 weeks was 26% less in OLD-fed mice than in HFD-fed mice (660 versus 887 kcal). The OLD group also showed significantly lower values for total visceral (epididymal, mesenteric, perirenal, and retroperitoneal) fat-pad weights (−72%, P < 0.05) than those observed in the HFD group. Histological sections from the epididymal adipose tissue of HFD-fed mice presented a greater adipocyte diameter as compared with CD-fed mice; this change in the adipocyte size was significantly reversed by OLE supplementation (−47%, P < 0.05) (Figures 2(e) and 2(f)). OLE had greater effects on lowering body weight, food intake, total visceral fat-pad weights, and adipocyte size than did sibutramine (Figures 2(a)–2(f)).


Olive leaf extract attenuates obesity in high-fat diet-fed mice by modulating the expression of molecules involved in adipogenesis and thermogenesis.

Shen Y, Song SJ, Keum N, Park T - Evid Based Complement Alternat Med (2014)

Effects of OLE on body weight gain, food efficiency ratio (FER), and visceral fat-pad weights of HFD-fed mice. Mice were fed CD, HFD, OLD, or SD for 8 weeks. Changes in the (a) body weight, (b) body weight gain, (c) food intake, (d) FER, and (e) visceral fat-pad weights. (f) Histological analysis of the epididymal adipose tissue from each group was conducted to quantify the size of adipocyte. (Left) Representative pictures of hematoxylin-eosin (H&E)-stained fat cells from the mice epididymal adipose tissue (×100). (Right) Densitometric analysis of the average adipocyte diameter in the epididymal adipose tissue of mice. Values are presented as means ± SEM (n = 8). Mean values indicated with different letters indicate statistical significance (P < 0.05); FER = Body weight gain for experimental period (g)/food intake for experimental period (g).
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3927866&req=5

fig2: Effects of OLE on body weight gain, food efficiency ratio (FER), and visceral fat-pad weights of HFD-fed mice. Mice were fed CD, HFD, OLD, or SD for 8 weeks. Changes in the (a) body weight, (b) body weight gain, (c) food intake, (d) FER, and (e) visceral fat-pad weights. (f) Histological analysis of the epididymal adipose tissue from each group was conducted to quantify the size of adipocyte. (Left) Representative pictures of hematoxylin-eosin (H&E)-stained fat cells from the mice epididymal adipose tissue (×100). (Right) Densitometric analysis of the average adipocyte diameter in the epididymal adipose tissue of mice. Values are presented as means ± SEM (n = 8). Mean values indicated with different letters indicate statistical significance (P < 0.05); FER = Body weight gain for experimental period (g)/food intake for experimental period (g).
Mentions: HFD-fed mice showed significant increases in final body weight (38%, P < 0.05) and cumulative body weight gain (162%, P < 0.05) as compared with CD-fed mice. OLD-fed mice showed a 37% reduction in final body weight and an 80% decrease in body weight gain as compared with HFD-fed mice (Figures 2(a) and 2(b)). The food intake was also significantly lower in OLD-fed mice (−26%, P < 0.05) than in HFD-fed mice during the 8-week feeding period. The food efficiency ratio (FER) (−74%, P < 0.05) of OLD-fed mice was significantly lower than that of HFD-fed mice (Figures 2(c) and 2(d)). The cumulative caloric intake over 8 weeks was 26% less in OLD-fed mice than in HFD-fed mice (660 versus 887 kcal). The OLD group also showed significantly lower values for total visceral (epididymal, mesenteric, perirenal, and retroperitoneal) fat-pad weights (−72%, P < 0.05) than those observed in the HFD group. Histological sections from the epididymal adipose tissue of HFD-fed mice presented a greater adipocyte diameter as compared with CD-fed mice; this change in the adipocyte size was significantly reversed by OLE supplementation (−47%, P < 0.05) (Figures 2(e) and 2(f)). OLE had greater effects on lowering body weight, food intake, total visceral fat-pad weights, and adipocyte size than did sibutramine (Figures 2(a)–2(f)).

Bottom Line: OLD-fed mice showed significantly reduced body weight gain, visceral fat-pad weights, and plasma lipid levels as compared with HFD-fed mice.OLE significantly reversed the HFD-induced upregulation of WNT10b- and galanin-mediated signaling molecules and key adipogenic genes (PPAR γ , C/EBP α , CD36, FAS, and leptin) in the epididymal adipose tissue of HFD-fed mice.Furthermore, the HFD-induced downregulation of thermogenic genes involved in uncoupled respiration (SIRT1, PGC1 α , and UCP1) and mitochondrial biogenesis (TFAM, NRF-1, and COX2) was also significantly reversed by OLE.

View Article: PubMed Central - PubMed

Affiliation: Department of Food and Nutrition, Brain Korea 21 PLUS Project, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-749, Republic of Korea.

ABSTRACT
The present study aimed to investigate whether olive leaf extract (OLE) prevents high-fat diet (HFD)-induced obesity in mice and to explore the underlying mechanisms. Mice were randomly divided into groups that received a chow diet (CD), HFD, or 0.15% OLE-supplemented diet (OLD) for 8 weeks. OLD-fed mice showed significantly reduced body weight gain, visceral fat-pad weights, and plasma lipid levels as compared with HFD-fed mice. OLE significantly reversed the HFD-induced upregulation of WNT10b- and galanin-mediated signaling molecules and key adipogenic genes (PPAR γ , C/EBP α , CD36, FAS, and leptin) in the epididymal adipose tissue of HFD-fed mice. Furthermore, the HFD-induced downregulation of thermogenic genes involved in uncoupled respiration (SIRT1, PGC1 α , and UCP1) and mitochondrial biogenesis (TFAM, NRF-1, and COX2) was also significantly reversed by OLE. These results suggest that OLE exerts beneficial effects against obesity by regulating the expression of genes involved in adipogenesis and thermogenesis in the visceral adipose tissue of HFD-fed mice.

No MeSH data available.


Related in: MedlinePlus