Porcine incisional hernia model: Evaluation of biologically derived intact extracellular matrix repairs.
Bottom Line: Mechanical remodeling of porcine-derived acellular dermal matrix was noted over time.Porcine-derived acellular dermal matrix elastic modulus and ultimate tensile stress were similar to fascia at 6 weeks.In this study, porcine-derived acellular dermal matrix-reinforced repairs provided more complete wound healing response compared with primary closure.
Affiliation: LifeCell Corporation, Inc., Branchburg, NJ, USA.
We compared fascial wounds repaired with non-cross-linked intact porcine-derived acellular dermal matrix versus primary closure in a large-animal hernia model. Incisional hernias were created in Yucatan pigs and repaired after 3 weeks via open technique with suture-only primary closure or intraperitoneally placed porcine-derived acellular dermal matrix. Progressive changes in mechanical and biological properties of porcine-derived acellular dermal matrix and repair sites were assessed. Porcine-derived acellular dermal matrix-repaired hernias of additional animals were evaluated 2 and 4 weeks post incision to assess porcine-derived acellular dermal matrix regenerative potential and biomechanical changes. Hernias repaired with primary closure showed substantially more scarring and bone hyperplasia along the incision line. Mechanical remodeling of porcine-derived acellular dermal matrix was noted over time. Porcine-derived acellular dermal matrix elastic modulus and ultimate tensile stress were similar to fascia at 6 weeks. The biology of porcine-derived acellular dermal matrix-reinforced animals was more similar to native abdominal wall versus that with primary closure. In this study, porcine-derived acellular dermal matrix-reinforced repairs provided more complete wound healing response compared with primary closure.
No MeSH data available.
Related in: MedlinePlus
License 1 - License 2 - License 3
Mentions: Only tissues from herniated animals were used in the microarray analysis; 404 probes were statistically differentially expressed at 6 weeks between the suture-only and PADM groups (t-test, p < 0.05). Hierarchical clustering was used to cluster gene profiles of the 404 differentially expressed genes. In hierarchical clustering, genes with similar expression patterns are grouped together and are connected by a series of branches, forming the clustering tree. Branches most closely connected have the most similar gene expression. Hierarchical clustering analysis demonstrated that the gene profile in time 0 animals (animals with no defects; biopsies harvested at time of incision creation) was more similar to PADM-reconstructed animals compared with the suture-only group. Among PADM-reconstructed animals, the week 6 time point showed the most similarity to the native state and the week 2 time point showed the most similarity to the herniated state (Figure 9). Table 2 contains the 10 most differentially expressed genes at 6 weeks in the study groups.
No MeSH data available.