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Porcine incisional hernia model: Evaluation of biologically derived intact extracellular matrix repairs.

Monteiro GA, Delossantos AI, Rodriguez NL, Patel P, Franz MG, Wagner CT - J Tissue Eng (2013)

Bottom Line: Mechanical remodeling of porcine-derived acellular dermal matrix was noted over time.Porcine-derived acellular dermal matrix elastic modulus and ultimate tensile stress were similar to fascia at 6 weeks.In this study, porcine-derived acellular dermal matrix-reinforced repairs provided more complete wound healing response compared with primary closure.

View Article: PubMed Central - PubMed

Affiliation: LifeCell Corporation, Inc., Branchburg, NJ, USA.

ABSTRACT
We compared fascial wounds repaired with non-cross-linked intact porcine-derived acellular dermal matrix versus primary closure in a large-animal hernia model. Incisional hernias were created in Yucatan pigs and repaired after 3 weeks via open technique with suture-only primary closure or intraperitoneally placed porcine-derived acellular dermal matrix. Progressive changes in mechanical and biological properties of porcine-derived acellular dermal matrix and repair sites were assessed. Porcine-derived acellular dermal matrix-repaired hernias of additional animals were evaluated 2 and 4 weeks post incision to assess porcine-derived acellular dermal matrix regenerative potential and biomechanical changes. Hernias repaired with primary closure showed substantially more scarring and bone hyperplasia along the incision line. Mechanical remodeling of porcine-derived acellular dermal matrix was noted over time. Porcine-derived acellular dermal matrix elastic modulus and ultimate tensile stress were similar to fascia at 6 weeks. The biology of porcine-derived acellular dermal matrix-reinforced animals was more similar to native abdominal wall versus that with primary closure. In this study, porcine-derived acellular dermal matrix-reinforced repairs provided more complete wound healing response compared with primary closure.

No MeSH data available.


Related in: MedlinePlus

Within the herniated group, Young’s modulus values were statistically different between all three time points (2 and 4 weeks, 2 and 6 weeks, 4 and 6 weeks; p < 0.0001, p < 0.0001, and p < 0.021, respectively). A statistically significant increase in Young’s modulus was noted between weeks 4 and 6. The crossed circles represent the mean value of  Young’s modulus for each group. At 4 weeks, all animals presented with hernias following the 3 weeks of incision. The x-axis represents two groups: presence of hernia and time of euthanization following repair. The y-axis represents Young’s modulus.
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fig6-2041731413508771: Within the herniated group, Young’s modulus values were statistically different between all three time points (2 and 4 weeks, 2 and 6 weeks, 4 and 6 weeks; p < 0.0001, p < 0.0001, and p < 0.021, respectively). A statistically significant increase in Young’s modulus was noted between weeks 4 and 6. The crossed circles represent the mean value of  Young’s modulus for each group. At 4 weeks, all animals presented with hernias following the 3 weeks of incision. The x-axis represents two groups: presence of hernia and time of euthanization following repair. The y-axis represents Young’s modulus.

Mentions: At 2 weeks, a decrease of approximately 40% in the median ultimate tensile strength was seen, while no changes in Young’s modulus were seen compared with day 0 values. At 4 and 6 weeks, the corresponding decreases versus day 0 values were approximately 84% and 81% in the median ultimate tensile strength and 70% and 65% for Young’s modulus, respectively. Statistical differences were confirmed in both the change in median ultimate tensile strength and Young’s modulus over time (Kruskal–Wallis test, p < 0.0001 for both) and for the differences in ultimate tensile strength between week 2 and weeks 4 and 6 (Mann–Whitney test, p < 0.0001 for both) (Figure 5). Young’s modulus was significantly different between all three time points (2 weeks and 4 weeks, 2 weeks and 6 weeks, 4 weeks and 6 weeks; p < 0.0001, p < 0.0001, and p < 0.021, respectively) (Figure 6). A significant increase in Young’s modulus was noted between weeks 4 and 6.


Porcine incisional hernia model: Evaluation of biologically derived intact extracellular matrix repairs.

Monteiro GA, Delossantos AI, Rodriguez NL, Patel P, Franz MG, Wagner CT - J Tissue Eng (2013)

Within the herniated group, Young’s modulus values were statistically different between all three time points (2 and 4 weeks, 2 and 6 weeks, 4 and 6 weeks; p < 0.0001, p < 0.0001, and p < 0.021, respectively). A statistically significant increase in Young’s modulus was noted between weeks 4 and 6. The crossed circles represent the mean value of  Young’s modulus for each group. At 4 weeks, all animals presented with hernias following the 3 weeks of incision. The x-axis represents two groups: presence of hernia and time of euthanization following repair. The y-axis represents Young’s modulus.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2 - License 3
Show All Figures
getmorefigures.php?uid=PMC3927864&req=5

fig6-2041731413508771: Within the herniated group, Young’s modulus values were statistically different between all three time points (2 and 4 weeks, 2 and 6 weeks, 4 and 6 weeks; p < 0.0001, p < 0.0001, and p < 0.021, respectively). A statistically significant increase in Young’s modulus was noted between weeks 4 and 6. The crossed circles represent the mean value of  Young’s modulus for each group. At 4 weeks, all animals presented with hernias following the 3 weeks of incision. The x-axis represents two groups: presence of hernia and time of euthanization following repair. The y-axis represents Young’s modulus.
Mentions: At 2 weeks, a decrease of approximately 40% in the median ultimate tensile strength was seen, while no changes in Young’s modulus were seen compared with day 0 values. At 4 and 6 weeks, the corresponding decreases versus day 0 values were approximately 84% and 81% in the median ultimate tensile strength and 70% and 65% for Young’s modulus, respectively. Statistical differences were confirmed in both the change in median ultimate tensile strength and Young’s modulus over time (Kruskal–Wallis test, p < 0.0001 for both) and for the differences in ultimate tensile strength between week 2 and weeks 4 and 6 (Mann–Whitney test, p < 0.0001 for both) (Figure 5). Young’s modulus was significantly different between all three time points (2 weeks and 4 weeks, 2 weeks and 6 weeks, 4 weeks and 6 weeks; p < 0.0001, p < 0.0001, and p < 0.021, respectively) (Figure 6). A significant increase in Young’s modulus was noted between weeks 4 and 6.

Bottom Line: Mechanical remodeling of porcine-derived acellular dermal matrix was noted over time.Porcine-derived acellular dermal matrix elastic modulus and ultimate tensile stress were similar to fascia at 6 weeks.In this study, porcine-derived acellular dermal matrix-reinforced repairs provided more complete wound healing response compared with primary closure.

View Article: PubMed Central - PubMed

Affiliation: LifeCell Corporation, Inc., Branchburg, NJ, USA.

ABSTRACT
We compared fascial wounds repaired with non-cross-linked intact porcine-derived acellular dermal matrix versus primary closure in a large-animal hernia model. Incisional hernias were created in Yucatan pigs and repaired after 3 weeks via open technique with suture-only primary closure or intraperitoneally placed porcine-derived acellular dermal matrix. Progressive changes in mechanical and biological properties of porcine-derived acellular dermal matrix and repair sites were assessed. Porcine-derived acellular dermal matrix-repaired hernias of additional animals were evaluated 2 and 4 weeks post incision to assess porcine-derived acellular dermal matrix regenerative potential and biomechanical changes. Hernias repaired with primary closure showed substantially more scarring and bone hyperplasia along the incision line. Mechanical remodeling of porcine-derived acellular dermal matrix was noted over time. Porcine-derived acellular dermal matrix elastic modulus and ultimate tensile stress were similar to fascia at 6 weeks. The biology of porcine-derived acellular dermal matrix-reinforced animals was more similar to native abdominal wall versus that with primary closure. In this study, porcine-derived acellular dermal matrix-reinforced repairs provided more complete wound healing response compared with primary closure.

No MeSH data available.


Related in: MedlinePlus