Porcine incisional hernia model: Evaluation of biologically derived intact extracellular matrix repairs.
Bottom Line: Mechanical remodeling of porcine-derived acellular dermal matrix was noted over time.Porcine-derived acellular dermal matrix elastic modulus and ultimate tensile stress were similar to fascia at 6 weeks.In this study, porcine-derived acellular dermal matrix-reinforced repairs provided more complete wound healing response compared with primary closure.
Affiliation: LifeCell Corporation, Inc., Branchburg, NJ, USA.
We compared fascial wounds repaired with non-cross-linked intact porcine-derived acellular dermal matrix versus primary closure in a large-animal hernia model. Incisional hernias were created in Yucatan pigs and repaired after 3 weeks via open technique with suture-only primary closure or intraperitoneally placed porcine-derived acellular dermal matrix. Progressive changes in mechanical and biological properties of porcine-derived acellular dermal matrix and repair sites were assessed. Porcine-derived acellular dermal matrix-repaired hernias of additional animals were evaluated 2 and 4 weeks post incision to assess porcine-derived acellular dermal matrix regenerative potential and biomechanical changes. Hernias repaired with primary closure showed substantially more scarring and bone hyperplasia along the incision line. Mechanical remodeling of porcine-derived acellular dermal matrix was noted over time. Porcine-derived acellular dermal matrix elastic modulus and ultimate tensile stress were similar to fascia at 6 weeks. The biology of porcine-derived acellular dermal matrix-reinforced animals was more similar to native abdominal wall versus that with primary closure. In this study, porcine-derived acellular dermal matrix-reinforced repairs provided more complete wound healing response compared with primary closure.
No MeSH data available.
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Mentions: At scheduled explant times (2, 4, and 6 weeks), animals were euthanized, skin flaps were raised, and the PADM was excised en bloc with surrounding abdominal wall muscles and tissue (Figure 2). Biopsies of tissue within and away from the PADM were taken and flash frozen in liquid nitrogen for gene expression evaluation. PADM size measurements were approximated and general observations were noted. Explants were shipped under Roswell Park Memorial Institute medium on wet ice for mechanical evaluation. On receipt of explants at the mechanical test facility, gross observations were rerecorded. The extent of PADM adhesion to the peritoneum was recorded. Any damage or disruptions in the tissue or wound site were also noted. PADM anchoring sutures were cut and the PADM was blunt dissected away from the peritoneum. Samples for mechanical testing were harvested from the freed PADM. Histopathology samples were harvested from three regions on the PADM: at the suture interface, within the herniation site, and outside the herniation site. Locations of mechanical samples on the PADM and relative to the abdominal wall incision were recorded. All mechanical samples were approximately 1 × 6 cm. Histology samples were approximately 1 × 1 cm.
No MeSH data available.