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Evaluation of an immunoassay-based algorithm for screening and identification of giardia and cryptosporidium antigens in human faecal specimens from saudi arabia.

Hawash Y - J Parasitol Res (2014)

Bottom Line: For Cryptosporidium, specificities of the ELISA and RIDA Quick Cryptosporidium kits were 95.71% and 100%, respectively.Both kits demonstrated sensitivity of 95%.In conclusion, this immunoassay-based algorithm can be used as routine test in diagnostic laboratories for screening and identification of a large number of samples.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Parasitology, National Liver Institute, Menoufia University, Shebin-El Koum 32513, Menoufia, Egypt ; Department of Medical Laboratory Science, College of Applied Medical Science, Taif University, Taif 2425, Saudi Arabia.

ABSTRACT
An immunoassay-based algorithm, involving three commercial kits, was introduced and evaluated for screening and identification of Giardia/Cryptosporidium antigens in human stool specimens. Initially, Giardia/Cryptosporidium Chek kit (TechLab), an enzyme-linked immunosorbent assay (ELISA), was adopted for screening. The ELISA-positive reactions were subsequently characterised by RIDA Quick Giardia and RIDA Quick Cryptosporidium immunochromatographic kits (R-Biopharm). A gold standard test comprising PCR and microscopy was used for preparing control samples. Performance of individual kits was tested against these samples which included 50 Giardia-positive, 40 Cryptosporidium-positive, and 70 Cryptosporidium/Giardia-negative. For Cryptosporidium, specificities of the ELISA and RIDA Quick Cryptosporidium kits were 95.71% and 100%, respectively. Both kits demonstrated sensitivity of 95%. For Giardia, the ELISA and RIDA Quick Giardia kits showed sensitivities of 100% and 97.5%, respectively. Specificities obtained by the ELISA and RIDA Quick Giardia were 95.7% and 100%, respectively. Based on the results of two reference PCRs, on 250 random samples, the algorithm exhibited sensitivity, specificity, positive predictive value, and negative predictive value of 97.06%, 100.00%, 100.00%, and 98.91%, respectively. In conclusion, this immunoassay-based algorithm can be used as routine test in diagnostic laboratories for screening and identification of a large number of samples.

No MeSH data available.


Related in: MedlinePlus

Representative bright field microscopic pictures for the Cryptosporidium oocysts stained with modified Ziehl-Neelsen dye with ×200 magnification.
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Related In: Results  -  Collection


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fig1: Representative bright field microscopic pictures for the Cryptosporidium oocysts stained with modified Ziehl-Neelsen dye with ×200 magnification.

Mentions: Based on the combined gold standard test-results on the preceding 160 stool samples, three positive control groups were assigned. Samples were diagnosed as positives by microscopy (see Figure 1) and the two PCR assays were selected as a positive control group (group-1).


Evaluation of an immunoassay-based algorithm for screening and identification of giardia and cryptosporidium antigens in human faecal specimens from saudi arabia.

Hawash Y - J Parasitol Res (2014)

Representative bright field microscopic pictures for the Cryptosporidium oocysts stained with modified Ziehl-Neelsen dye with ×200 magnification.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3927860&req=5

fig1: Representative bright field microscopic pictures for the Cryptosporidium oocysts stained with modified Ziehl-Neelsen dye with ×200 magnification.
Mentions: Based on the combined gold standard test-results on the preceding 160 stool samples, three positive control groups were assigned. Samples were diagnosed as positives by microscopy (see Figure 1) and the two PCR assays were selected as a positive control group (group-1).

Bottom Line: For Cryptosporidium, specificities of the ELISA and RIDA Quick Cryptosporidium kits were 95.71% and 100%, respectively.Both kits demonstrated sensitivity of 95%.In conclusion, this immunoassay-based algorithm can be used as routine test in diagnostic laboratories for screening and identification of a large number of samples.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Parasitology, National Liver Institute, Menoufia University, Shebin-El Koum 32513, Menoufia, Egypt ; Department of Medical Laboratory Science, College of Applied Medical Science, Taif University, Taif 2425, Saudi Arabia.

ABSTRACT
An immunoassay-based algorithm, involving three commercial kits, was introduced and evaluated for screening and identification of Giardia/Cryptosporidium antigens in human stool specimens. Initially, Giardia/Cryptosporidium Chek kit (TechLab), an enzyme-linked immunosorbent assay (ELISA), was adopted for screening. The ELISA-positive reactions were subsequently characterised by RIDA Quick Giardia and RIDA Quick Cryptosporidium immunochromatographic kits (R-Biopharm). A gold standard test comprising PCR and microscopy was used for preparing control samples. Performance of individual kits was tested against these samples which included 50 Giardia-positive, 40 Cryptosporidium-positive, and 70 Cryptosporidium/Giardia-negative. For Cryptosporidium, specificities of the ELISA and RIDA Quick Cryptosporidium kits were 95.71% and 100%, respectively. Both kits demonstrated sensitivity of 95%. For Giardia, the ELISA and RIDA Quick Giardia kits showed sensitivities of 100% and 97.5%, respectively. Specificities obtained by the ELISA and RIDA Quick Giardia were 95.7% and 100%, respectively. Based on the results of two reference PCRs, on 250 random samples, the algorithm exhibited sensitivity, specificity, positive predictive value, and negative predictive value of 97.06%, 100.00%, 100.00%, and 98.91%, respectively. In conclusion, this immunoassay-based algorithm can be used as routine test in diagnostic laboratories for screening and identification of a large number of samples.

No MeSH data available.


Related in: MedlinePlus