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Diverse effects of lead nitrate on the proliferation, differentiation, and gene expression of stem cells isolated from a dental origin.

Abdullah M, Rahman FA, Gnanasegaran N, Govindasamy V, Abu Kasim NH, Musa S - ScientificWorldJournal (2014)

Bottom Line: We found that Pb(2+) treatment altered the morphology and adhesion of the cells in a dose-dependent manner.There were no significant changes in terms of cell surface phenotypes.Gene expression studies revealed a constant expression of key markers associated with stemness (Oct 4, Rex 1) and DNA repair enzyme markers, but downregulation occurred with some ectoderm and endoderm markers, demonstrating an irregular and untimely differentiation trail.

View Article: PubMed Central - PubMed

Affiliation: Department of Conservative Dentistry, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, Malaysia.

ABSTRACT
Lead (Pb(2+)) exposure continues to be a significant public health problem. Therefore, it is vital to have a continuous epidemiological dataset for a better understanding of Pb(2+) toxicity. In the present study, we have exposed stem cells isolated from deciduous and permanent teeth, periodontal ligament, and bone marrow to five different types of Pb(2+) concentrations (160, 80, 40, 20, and 10 µM) for 24 hours to identify the adverse effects of Pb(2+) on the proliferation, differentiation, and gene expression on these cell lines. We found that Pb(2+) treatment altered the morphology and adhesion of the cells in a dose-dependent manner. There were no significant changes in terms of cell surface phenotypes. Cells exposed to Pb(2+) continued to differentiate into chondrogenesis and adipogenesis, and a severe downregulation was observed in osteogenesis. Gene expression studies revealed a constant expression of key markers associated with stemness (Oct 4, Rex 1) and DNA repair enzyme markers, but downregulation occurred with some ectoderm and endoderm markers, demonstrating an irregular and untimely differentiation trail. Our study revealed for the first time that Pb(2+) exposure not only affects the phenotypic characteristics but also induces significant alteration in the differentiation and gene expression in the cells.

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Related in: MedlinePlus

In vitro differential potentiality of bone marrow stem cells (BM-MSCs), deciduous stem cells (SCDs), periodontal ligament stem cells (PDLs), and permanent stem cells (DPSCs) in presence of various concentrations of Pb2+. Osteogenesis was confirmed by mineralized matrix deposition stained with von Kossa staining at day 21. Adipogenesis was detected by neutral oil droplet formation stained with Oil Red O at day 21. Chondogenesis was detected by the presence of proteoglycans stained with alcian blue dye at day 21. The results represent average of 3 independent culture replicates. A representative photomicrograph was given for each experiment.
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fig3: In vitro differential potentiality of bone marrow stem cells (BM-MSCs), deciduous stem cells (SCDs), periodontal ligament stem cells (PDLs), and permanent stem cells (DPSCs) in presence of various concentrations of Pb2+. Osteogenesis was confirmed by mineralized matrix deposition stained with von Kossa staining at day 21. Adipogenesis was detected by neutral oil droplet formation stained with Oil Red O at day 21. Chondogenesis was detected by the presence of proteoglycans stained with alcian blue dye at day 21. The results represent average of 3 independent culture replicates. A representative photomicrograph was given for each experiment.

Mentions: We investigated the potential of BM-MSCs, SCDs, DPSCs, and PDLs to differentiate into osteogenic, chondrogenic, and adipogenic lineages at various concentrations of Pb2+ (Figure 3). Osteogenic differentiation was confirmed by the detection of the silver stained mineralized matrix in the control samples. While SCDs, DPSCs, and PDLs showed a weak deposition of calcium in the mineralized matrix only at the pretreatment with 160 μM of Pb2+, BM-MSCs preexposed to Pb2+ concentration of 40 μM have started to show a similar observation (Figure 3). Chondrogenic differentiation was detected by the presence of proteoglycans stained with alcian blue at day 21 in BM-MSCs, SCDs, PDLs, and DPSCs.


Diverse effects of lead nitrate on the proliferation, differentiation, and gene expression of stem cells isolated from a dental origin.

Abdullah M, Rahman FA, Gnanasegaran N, Govindasamy V, Abu Kasim NH, Musa S - ScientificWorldJournal (2014)

In vitro differential potentiality of bone marrow stem cells (BM-MSCs), deciduous stem cells (SCDs), periodontal ligament stem cells (PDLs), and permanent stem cells (DPSCs) in presence of various concentrations of Pb2+. Osteogenesis was confirmed by mineralized matrix deposition stained with von Kossa staining at day 21. Adipogenesis was detected by neutral oil droplet formation stained with Oil Red O at day 21. Chondogenesis was detected by the presence of proteoglycans stained with alcian blue dye at day 21. The results represent average of 3 independent culture replicates. A representative photomicrograph was given for each experiment.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3927845&req=5

fig3: In vitro differential potentiality of bone marrow stem cells (BM-MSCs), deciduous stem cells (SCDs), periodontal ligament stem cells (PDLs), and permanent stem cells (DPSCs) in presence of various concentrations of Pb2+. Osteogenesis was confirmed by mineralized matrix deposition stained with von Kossa staining at day 21. Adipogenesis was detected by neutral oil droplet formation stained with Oil Red O at day 21. Chondogenesis was detected by the presence of proteoglycans stained with alcian blue dye at day 21. The results represent average of 3 independent culture replicates. A representative photomicrograph was given for each experiment.
Mentions: We investigated the potential of BM-MSCs, SCDs, DPSCs, and PDLs to differentiate into osteogenic, chondrogenic, and adipogenic lineages at various concentrations of Pb2+ (Figure 3). Osteogenic differentiation was confirmed by the detection of the silver stained mineralized matrix in the control samples. While SCDs, DPSCs, and PDLs showed a weak deposition of calcium in the mineralized matrix only at the pretreatment with 160 μM of Pb2+, BM-MSCs preexposed to Pb2+ concentration of 40 μM have started to show a similar observation (Figure 3). Chondrogenic differentiation was detected by the presence of proteoglycans stained with alcian blue at day 21 in BM-MSCs, SCDs, PDLs, and DPSCs.

Bottom Line: We found that Pb(2+) treatment altered the morphology and adhesion of the cells in a dose-dependent manner.There were no significant changes in terms of cell surface phenotypes.Gene expression studies revealed a constant expression of key markers associated with stemness (Oct 4, Rex 1) and DNA repair enzyme markers, but downregulation occurred with some ectoderm and endoderm markers, demonstrating an irregular and untimely differentiation trail.

View Article: PubMed Central - PubMed

Affiliation: Department of Conservative Dentistry, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, Malaysia.

ABSTRACT
Lead (Pb(2+)) exposure continues to be a significant public health problem. Therefore, it is vital to have a continuous epidemiological dataset for a better understanding of Pb(2+) toxicity. In the present study, we have exposed stem cells isolated from deciduous and permanent teeth, periodontal ligament, and bone marrow to five different types of Pb(2+) concentrations (160, 80, 40, 20, and 10 µM) for 24 hours to identify the adverse effects of Pb(2+) on the proliferation, differentiation, and gene expression on these cell lines. We found that Pb(2+) treatment altered the morphology and adhesion of the cells in a dose-dependent manner. There were no significant changes in terms of cell surface phenotypes. Cells exposed to Pb(2+) continued to differentiate into chondrogenesis and adipogenesis, and a severe downregulation was observed in osteogenesis. Gene expression studies revealed a constant expression of key markers associated with stemness (Oct 4, Rex 1) and DNA repair enzyme markers, but downregulation occurred with some ectoderm and endoderm markers, demonstrating an irregular and untimely differentiation trail. Our study revealed for the first time that Pb(2+) exposure not only affects the phenotypic characteristics but also induces significant alteration in the differentiation and gene expression in the cells.

Show MeSH
Related in: MedlinePlus