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Oligofructose supplementation during pregnancy and lactation impairs offspring development and alters the intestinal properties of 21-d-old pups.

Mennitti LV, Oyama LM, de Oliveira JL, Hachul AC, Santamarina AB, de Santana AA, Okuda MH, Ribeiro EB, do Nascimento CM, Pisani LP - Lipids Health Dis (2014)

Bottom Line: On the other hand, prebiotics can alter the intestinal environment, reducing serum lipopolysaccharides (LPS) concentrations.Data were expressed as mean ± standard error of the mean and the statistical analysis was realized by ANOVA two-way and ANOVA for repeated measures. p < 0.05 was considered significant.We observed that the oligofructose (10%) supplementation during pregnancy and lactation reduced body weight, body weight gain, length and serum FFA in the CF and TF group compared to C and T group respectively, of the 21-day-old offspring, accompanied by an increase in serum LPS and genomic DNA levels of lactobacillus spp. on faeces of the CF group in relation to C group.

View Article: PubMed Central - HTML - PubMed

Affiliation: Departamento de Biociências, Instituto de Saúde e Sociedade, Universidade Federal de São Paulo, Rua Silva Jardim, 136, Térreo, Vila Mathias, Santos, SP, Brazil. lucianapisani@hotmail.com.

ABSTRACT

Background: Previously, we showed that the intake of trans fatty acids during pregnancy and lactation triggers a pro-inflammatory status in the offspring. On the other hand, prebiotics can alter the intestinal environment, reducing serum lipopolysaccharides (LPS) concentrations. This study evaluated the effect of the oligofructose 10% diet supplementation in the presence or absence of hydrogenated vegetable fat during pregnancy and lactation on the development, endotoxemia and bacterial composition of 21-d-old offspring.

Methods: On the first day of pregnancy rats were divided into four groups: control diet (C), control diet supplemented with 10% oligofructose (CF), diet enriched with hydrogenated vegetable fat, rich in TFA (T) or diet enriched with hydrogenated vegetable fat supplemented with 10% oligofructose (TF). Diets were maintained during pregnancy and lactation. At birth, 7th, 14th and 21th, pups were weighed and length was measured. Serum concentrations of LPS and free fatty acids (FFA) were performed by specific kits. Bacterial DNA present in faeces was determined by real-time PCR. Data were expressed as mean ± standard error of the mean and the statistical analysis was realized by ANOVA two-way and ANOVA for repeated measures. p < 0.05 was considered significant.

Results: We observed that the oligofructose (10%) supplementation during pregnancy and lactation reduced body weight, body weight gain, length and serum FFA in the CF and TF group compared to C and T group respectively, of the 21-day-old offspring, accompanied by an increase in serum LPS and genomic DNA levels of lactobacillus spp. on faeces of the CF group in relation to C group.

Conclusion: In conclusion, dam's diet supplementation with 10% of oligofructose during pregnancy and lactation, independent of addition with hydrogenated vegetable fat, harms the offspring development, alters the bacterial composition and increases the serum concentrations of lipopolysaccharides in 21d-old pups.

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Related in: MedlinePlus

Serum concentration of lipopolysaccharides (LPS) (A) and serum concentration of free fatty acids (FFA) (B). C – offspring of dams fed control diet; CF –offspring of dams fed control diet supplemented with oligofructose; T – offspring of dams fed diet enriched with hydrogenated vegetable fat; TF – offspring of dams fed diet enriched with hydrogenated vegetable fat supplemented with oligofructose. The number in parentheses refers to the sample value. Data are means ± SEMs. *p ≤ 0.05 versus C. &p ≤ 0.05 versus CF. $p ≤ 0.05 versus T. #p ≤ 0.05 versus TF.
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Figure 2: Serum concentration of lipopolysaccharides (LPS) (A) and serum concentration of free fatty acids (FFA) (B). C – offspring of dams fed control diet; CF –offspring of dams fed control diet supplemented with oligofructose; T – offspring of dams fed diet enriched with hydrogenated vegetable fat; TF – offspring of dams fed diet enriched with hydrogenated vegetable fat supplemented with oligofructose. The number in parentheses refers to the sample value. Data are means ± SEMs. *p ≤ 0.05 versus C. &p ≤ 0.05 versus CF. $p ≤ 0.05 versus T. #p ≤ 0.05 versus TF.

Mentions: The serum concentration of LPS in the CF group was significantly higher than the C group (p = 0.05) (Figure 2A).


Oligofructose supplementation during pregnancy and lactation impairs offspring development and alters the intestinal properties of 21-d-old pups.

Mennitti LV, Oyama LM, de Oliveira JL, Hachul AC, Santamarina AB, de Santana AA, Okuda MH, Ribeiro EB, do Nascimento CM, Pisani LP - Lipids Health Dis (2014)

Serum concentration of lipopolysaccharides (LPS) (A) and serum concentration of free fatty acids (FFA) (B). C – offspring of dams fed control diet; CF –offspring of dams fed control diet supplemented with oligofructose; T – offspring of dams fed diet enriched with hydrogenated vegetable fat; TF – offspring of dams fed diet enriched with hydrogenated vegetable fat supplemented with oligofructose. The number in parentheses refers to the sample value. Data are means ± SEMs. *p ≤ 0.05 versus C. &p ≤ 0.05 versus CF. $p ≤ 0.05 versus T. #p ≤ 0.05 versus TF.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3927778&req=5

Figure 2: Serum concentration of lipopolysaccharides (LPS) (A) and serum concentration of free fatty acids (FFA) (B). C – offspring of dams fed control diet; CF –offspring of dams fed control diet supplemented with oligofructose; T – offspring of dams fed diet enriched with hydrogenated vegetable fat; TF – offspring of dams fed diet enriched with hydrogenated vegetable fat supplemented with oligofructose. The number in parentheses refers to the sample value. Data are means ± SEMs. *p ≤ 0.05 versus C. &p ≤ 0.05 versus CF. $p ≤ 0.05 versus T. #p ≤ 0.05 versus TF.
Mentions: The serum concentration of LPS in the CF group was significantly higher than the C group (p = 0.05) (Figure 2A).

Bottom Line: On the other hand, prebiotics can alter the intestinal environment, reducing serum lipopolysaccharides (LPS) concentrations.Data were expressed as mean ± standard error of the mean and the statistical analysis was realized by ANOVA two-way and ANOVA for repeated measures. p < 0.05 was considered significant.We observed that the oligofructose (10%) supplementation during pregnancy and lactation reduced body weight, body weight gain, length and serum FFA in the CF and TF group compared to C and T group respectively, of the 21-day-old offspring, accompanied by an increase in serum LPS and genomic DNA levels of lactobacillus spp. on faeces of the CF group in relation to C group.

View Article: PubMed Central - HTML - PubMed

Affiliation: Departamento de Biociências, Instituto de Saúde e Sociedade, Universidade Federal de São Paulo, Rua Silva Jardim, 136, Térreo, Vila Mathias, Santos, SP, Brazil. lucianapisani@hotmail.com.

ABSTRACT

Background: Previously, we showed that the intake of trans fatty acids during pregnancy and lactation triggers a pro-inflammatory status in the offspring. On the other hand, prebiotics can alter the intestinal environment, reducing serum lipopolysaccharides (LPS) concentrations. This study evaluated the effect of the oligofructose 10% diet supplementation in the presence or absence of hydrogenated vegetable fat during pregnancy and lactation on the development, endotoxemia and bacterial composition of 21-d-old offspring.

Methods: On the first day of pregnancy rats were divided into four groups: control diet (C), control diet supplemented with 10% oligofructose (CF), diet enriched with hydrogenated vegetable fat, rich in TFA (T) or diet enriched with hydrogenated vegetable fat supplemented with 10% oligofructose (TF). Diets were maintained during pregnancy and lactation. At birth, 7th, 14th and 21th, pups were weighed and length was measured. Serum concentrations of LPS and free fatty acids (FFA) were performed by specific kits. Bacterial DNA present in faeces was determined by real-time PCR. Data were expressed as mean ± standard error of the mean and the statistical analysis was realized by ANOVA two-way and ANOVA for repeated measures. p < 0.05 was considered significant.

Results: We observed that the oligofructose (10%) supplementation during pregnancy and lactation reduced body weight, body weight gain, length and serum FFA in the CF and TF group compared to C and T group respectively, of the 21-day-old offspring, accompanied by an increase in serum LPS and genomic DNA levels of lactobacillus spp. on faeces of the CF group in relation to C group.

Conclusion: In conclusion, dam's diet supplementation with 10% of oligofructose during pregnancy and lactation, independent of addition with hydrogenated vegetable fat, harms the offspring development, alters the bacterial composition and increases the serum concentrations of lipopolysaccharides in 21d-old pups.

Show MeSH
Related in: MedlinePlus