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Nutraceutical potential of tinctures from fruits, green husks, and leaves of Juglans regia L.

Gawlik-Dziki U, Durak A, Pecio Ł, Kowalska I - ScientificWorldJournal (2014)

Bottom Line: The highest reducing power was found for F3 and F25 (EC50 = 32.47 ± 1.53 and 36.07 ± 1.72 mg FM/mL, resp.).Tested tinctures showed relatively high antiradical activity-EC50 values ranged from 100.56 ± 5.03 to 129.04 ± 6.45 mg FM/mL for L and F25, respectively.The results obtained suggest that J. regia can be a source of bioactive compounds with antioxidant properties.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Food Chemistry, University of Life Sciences, Skromna Street 8, 20-704 Lublin, Poland.

ABSTRACT
The aim of this study was to assess the phenolic composition and nutraceutical potential of tinctures from fruits in two stages of maturity (F3, younger; F25, older), green husks (GH), and leaves (L) of Juglans regia L. In all extracts gallic, protocatechuic, 3-caffeoylquinic, 3-ρ-coumaroylquinic, 4-caffeoylquinic, 4-ρ-coumaroylquinic, and ρ -coumaric acids and quercetin-3-O-deoxyhexoside were detected using UPLC-MS technique. Caffeic acid hexoside I and quercetin-3-O-deoxyhexoside I have been identified in GH tincture. The highest ability to chelate Fe(2+) was observed for GH tincture (EC50 = 71.01 ± 3.55 mg FM/mL), whereas the lowest was observed (EC50 = 131.06 ± 6.55 mg FM/mL) for F3 tincture. The highest reducing power was found for F3 and F25 (EC50 = 32.47 ± 1.53 and 36.07 ± 1.72 mg FM/mL, resp.). Ability of tinctures to prevent lipids against oxidation was relatively low. The highest activity (EC50 = 126.49 ± 6.32 mg FM/mL) was determined for F25. Tested tinctures showed relatively high antiradical activity-EC50 values ranged from 100.56 ± 5.03 to 129.04 ± 6.45 mg FM/mL for L and F25, respectively. The results obtained suggest that J. regia can be a source of bioactive compounds with antioxidant properties.

Show MeSH
Comparison of CAT-activatory abilities of tinctures from different J. regia parts. F3: extract from walnut harvested 3.07.2012, F25: extract from walnut harvested 25.07.2012, GH: extract from green husks of walnut, and L: extract from walnut leaves. Bars (means) followed by the different letters differ significantly (Tukey's test, P < 0.05).
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fig4: Comparison of CAT-activatory abilities of tinctures from different J. regia parts. F3: extract from walnut harvested 3.07.2012, F25: extract from walnut harvested 25.07.2012, GH: extract from green husks of walnut, and L: extract from walnut leaves. Bars (means) followed by the different letters differ significantly (Tukey's test, P < 0.05).

Mentions: In the present study, the ability of extracts to increase CAT activity was investigated (Figure 4). It was observed that best CAT activators were tinctures from the leaves and walnut green husks, increasing CAT activity turn notify 14.01% and 13.02%, slightly weaker activity was observed for tinctures of J. regia fruits at both stages of maturity.


Nutraceutical potential of tinctures from fruits, green husks, and leaves of Juglans regia L.

Gawlik-Dziki U, Durak A, Pecio Ł, Kowalska I - ScientificWorldJournal (2014)

Comparison of CAT-activatory abilities of tinctures from different J. regia parts. F3: extract from walnut harvested 3.07.2012, F25: extract from walnut harvested 25.07.2012, GH: extract from green husks of walnut, and L: extract from walnut leaves. Bars (means) followed by the different letters differ significantly (Tukey's test, P < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3927754&req=5

fig4: Comparison of CAT-activatory abilities of tinctures from different J. regia parts. F3: extract from walnut harvested 3.07.2012, F25: extract from walnut harvested 25.07.2012, GH: extract from green husks of walnut, and L: extract from walnut leaves. Bars (means) followed by the different letters differ significantly (Tukey's test, P < 0.05).
Mentions: In the present study, the ability of extracts to increase CAT activity was investigated (Figure 4). It was observed that best CAT activators were tinctures from the leaves and walnut green husks, increasing CAT activity turn notify 14.01% and 13.02%, slightly weaker activity was observed for tinctures of J. regia fruits at both stages of maturity.

Bottom Line: The highest reducing power was found for F3 and F25 (EC50 = 32.47 ± 1.53 and 36.07 ± 1.72 mg FM/mL, resp.).Tested tinctures showed relatively high antiradical activity-EC50 values ranged from 100.56 ± 5.03 to 129.04 ± 6.45 mg FM/mL for L and F25, respectively.The results obtained suggest that J. regia can be a source of bioactive compounds with antioxidant properties.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Food Chemistry, University of Life Sciences, Skromna Street 8, 20-704 Lublin, Poland.

ABSTRACT
The aim of this study was to assess the phenolic composition and nutraceutical potential of tinctures from fruits in two stages of maturity (F3, younger; F25, older), green husks (GH), and leaves (L) of Juglans regia L. In all extracts gallic, protocatechuic, 3-caffeoylquinic, 3-ρ-coumaroylquinic, 4-caffeoylquinic, 4-ρ-coumaroylquinic, and ρ -coumaric acids and quercetin-3-O-deoxyhexoside were detected using UPLC-MS technique. Caffeic acid hexoside I and quercetin-3-O-deoxyhexoside I have been identified in GH tincture. The highest ability to chelate Fe(2+) was observed for GH tincture (EC50 = 71.01 ± 3.55 mg FM/mL), whereas the lowest was observed (EC50 = 131.06 ± 6.55 mg FM/mL) for F3 tincture. The highest reducing power was found for F3 and F25 (EC50 = 32.47 ± 1.53 and 36.07 ± 1.72 mg FM/mL, resp.). Ability of tinctures to prevent lipids against oxidation was relatively low. The highest activity (EC50 = 126.49 ± 6.32 mg FM/mL) was determined for F25. Tested tinctures showed relatively high antiradical activity-EC50 values ranged from 100.56 ± 5.03 to 129.04 ± 6.45 mg FM/mL for L and F25, respectively. The results obtained suggest that J. regia can be a source of bioactive compounds with antioxidant properties.

Show MeSH