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Nutraceutical potential of tinctures from fruits, green husks, and leaves of Juglans regia L.

Gawlik-Dziki U, Durak A, Pecio Ł, Kowalska I - ScientificWorldJournal (2014)

Bottom Line: The highest reducing power was found for F3 and F25 (EC50 = 32.47 ± 1.53 and 36.07 ± 1.72 mg FM/mL, resp.).Tested tinctures showed relatively high antiradical activity-EC50 values ranged from 100.56 ± 5.03 to 129.04 ± 6.45 mg FM/mL for L and F25, respectively.The results obtained suggest that J. regia can be a source of bioactive compounds with antioxidant properties.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Food Chemistry, University of Life Sciences, Skromna Street 8, 20-704 Lublin, Poland.

ABSTRACT
The aim of this study was to assess the phenolic composition and nutraceutical potential of tinctures from fruits in two stages of maturity (F3, younger; F25, older), green husks (GH), and leaves (L) of Juglans regia L. In all extracts gallic, protocatechuic, 3-caffeoylquinic, 3-ρ-coumaroylquinic, 4-caffeoylquinic, 4-ρ-coumaroylquinic, and ρ -coumaric acids and quercetin-3-O-deoxyhexoside were detected using UPLC-MS technique. Caffeic acid hexoside I and quercetin-3-O-deoxyhexoside I have been identified in GH tincture. The highest ability to chelate Fe(2+) was observed for GH tincture (EC50 = 71.01 ± 3.55 mg FM/mL), whereas the lowest was observed (EC50 = 131.06 ± 6.55 mg FM/mL) for F3 tincture. The highest reducing power was found for F3 and F25 (EC50 = 32.47 ± 1.53 and 36.07 ± 1.72 mg FM/mL, resp.). Ability of tinctures to prevent lipids against oxidation was relatively low. The highest activity (EC50 = 126.49 ± 6.32 mg FM/mL) was determined for F25. Tested tinctures showed relatively high antiradical activity-EC50 values ranged from 100.56 ± 5.03 to 129.04 ± 6.45 mg FM/mL for L and F25, respectively. The results obtained suggest that J. regia can be a source of bioactive compounds with antioxidant properties.

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UPLC chromatograms of tinctures from different parts of J. regia. L: extract from walnut leaves, F3: extract from walnut harvested 3.07.2012, F25: extract from walnut harvested 25.07.2012, and GH: extract from green husks of walnut.
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fig1: UPLC chromatograms of tinctures from different parts of J. regia. L: extract from walnut leaves, F3: extract from walnut harvested 3.07.2012, F25: extract from walnut harvested 25.07.2012, and GH: extract from green husks of walnut.

Mentions: In all extracts prepared, the following eight phenolic compounds were detected: gallic, protocatechuic, 3-caffeoylquinic, 3-ρ-coumaroylquinic, 4-caffeoylquinic, 4-ρ-coumaroylquinic, and ρ-coumaric acids, as well as quercetin-3-O-deoxyhexoside. Furthermore, ethanolic extract of leaves is characterized by the greatest diversity of phenolic compounds from the tested samples, as presented in Table 1 and Figure 1.


Nutraceutical potential of tinctures from fruits, green husks, and leaves of Juglans regia L.

Gawlik-Dziki U, Durak A, Pecio Ł, Kowalska I - ScientificWorldJournal (2014)

UPLC chromatograms of tinctures from different parts of J. regia. L: extract from walnut leaves, F3: extract from walnut harvested 3.07.2012, F25: extract from walnut harvested 25.07.2012, and GH: extract from green husks of walnut.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3927754&req=5

fig1: UPLC chromatograms of tinctures from different parts of J. regia. L: extract from walnut leaves, F3: extract from walnut harvested 3.07.2012, F25: extract from walnut harvested 25.07.2012, and GH: extract from green husks of walnut.
Mentions: In all extracts prepared, the following eight phenolic compounds were detected: gallic, protocatechuic, 3-caffeoylquinic, 3-ρ-coumaroylquinic, 4-caffeoylquinic, 4-ρ-coumaroylquinic, and ρ-coumaric acids, as well as quercetin-3-O-deoxyhexoside. Furthermore, ethanolic extract of leaves is characterized by the greatest diversity of phenolic compounds from the tested samples, as presented in Table 1 and Figure 1.

Bottom Line: The highest reducing power was found for F3 and F25 (EC50 = 32.47 ± 1.53 and 36.07 ± 1.72 mg FM/mL, resp.).Tested tinctures showed relatively high antiradical activity-EC50 values ranged from 100.56 ± 5.03 to 129.04 ± 6.45 mg FM/mL for L and F25, respectively.The results obtained suggest that J. regia can be a source of bioactive compounds with antioxidant properties.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Food Chemistry, University of Life Sciences, Skromna Street 8, 20-704 Lublin, Poland.

ABSTRACT
The aim of this study was to assess the phenolic composition and nutraceutical potential of tinctures from fruits in two stages of maturity (F3, younger; F25, older), green husks (GH), and leaves (L) of Juglans regia L. In all extracts gallic, protocatechuic, 3-caffeoylquinic, 3-ρ-coumaroylquinic, 4-caffeoylquinic, 4-ρ-coumaroylquinic, and ρ -coumaric acids and quercetin-3-O-deoxyhexoside were detected using UPLC-MS technique. Caffeic acid hexoside I and quercetin-3-O-deoxyhexoside I have been identified in GH tincture. The highest ability to chelate Fe(2+) was observed for GH tincture (EC50 = 71.01 ± 3.55 mg FM/mL), whereas the lowest was observed (EC50 = 131.06 ± 6.55 mg FM/mL) for F3 tincture. The highest reducing power was found for F3 and F25 (EC50 = 32.47 ± 1.53 and 36.07 ± 1.72 mg FM/mL, resp.). Ability of tinctures to prevent lipids against oxidation was relatively low. The highest activity (EC50 = 126.49 ± 6.32 mg FM/mL) was determined for F25. Tested tinctures showed relatively high antiradical activity-EC50 values ranged from 100.56 ± 5.03 to 129.04 ± 6.45 mg FM/mL for L and F25, respectively. The results obtained suggest that J. regia can be a source of bioactive compounds with antioxidant properties.

Show MeSH