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Assessment of cellular viability on calcium sulphate/hydroxyapatite injectable scaffolds.

Alfotawi R, Naudi K, Dalby MJ, Tanner KE, McMahon JD, Ayoub A - J Tissue Eng (2013)

Bottom Line: The objective of this study was to investigate the visibility of loading of two types of commercially available cements, Cerament(™) Spine Support and Cerament Bone Void Filler with mesenchymal cells and cytokines (bone morphogenetic protein) to act as a biomimetic scaffolding for future clinical application.Determination of basic biocompatibility (cell viability) using methyl thiazolyl tetrazolium and live/dead assay was carried out using MG-63 cells at various time points.Results indicated that Cerament Spine Support was more biocompatible and that sequential injection of cement and then rabbit mesenchymal stromal cells into the tissue mimics is an optimal approach for clinical applications.

View Article: PubMed Central - PubMed

Affiliation: Glasgow Dental Hospital & School, University of Glasgow, Glasgow, UK.

ABSTRACT
Cements for maxillofacial reconstruction of jaw defects through calcification of rotated muscle have been tested. The objective of this study was to investigate the visibility of loading of two types of commercially available cements, Cerament(™) Spine Support and Cerament Bone Void Filler with mesenchymal cells and cytokines (bone morphogenetic protein) to act as a biomimetic scaffolding for future clinical application. Determination of basic biocompatibility (cell viability) using methyl thiazolyl tetrazolium and live/dead assay was carried out using MG-63 cells at various time points. Next, in order to inform potential subsequent in vivo experiments, a collagen tissue mimic was used for characterization of rabbit mesenchymal stromal cells using immunofluorescent cytoskeleton staining, and simultaneous and then sequential injection of Cerament Spine Support cement and cells into collagen gels. Results indicated that Cerament Spine Support was more biocompatible and that sequential injection of cement and then rabbit mesenchymal stromal cells into the tissue mimics is an optimal approach for clinical applications.

No MeSH data available.


Related in: MedlinePlus

Scanning electron microscopy image showing rMSC (arrows) at day 1 of cell culture. The cell is covering micropore and is adherent onto the surface of the Cerament Spine Support cement (scale bar = 1 µm).rMSCs: rabbit mesenchymal stromal cells.
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fig6-2041731413509645: Scanning electron microscopy image showing rMSC (arrows) at day 1 of cell culture. The cell is covering micropore and is adherent onto the surface of the Cerament Spine Support cement (scale bar = 1 µm).rMSCs: rabbit mesenchymal stromal cells.

Mentions: A large number of rMSCs were adherent and were clearly visible on the surface of the Cerament Spine Support cement at all time points. At day 1, the rMSCs had started to adhere to the surface of the material and interact with the cement crystals (Figure 6). After day 3 of rMSC culture, there were areas where cells had proliferated well and aggregated with each other. Cells attained a typical size (60–160 µm in length), and they were polygonal or fusiform in shape (Figure 7). These cells had pseudopodia interacting with the crystals of the CS/HA cement (Figure 8). Cells showed accompanying filamentous fibres forming on the surface; this may be evidence for extracellular matrix production (Figure 8). After ascertaining that the surfaces supported rMSC growth, we moved to the three-dimensional (3D) model.


Assessment of cellular viability on calcium sulphate/hydroxyapatite injectable scaffolds.

Alfotawi R, Naudi K, Dalby MJ, Tanner KE, McMahon JD, Ayoub A - J Tissue Eng (2013)

Scanning electron microscopy image showing rMSC (arrows) at day 1 of cell culture. The cell is covering micropore and is adherent onto the surface of the Cerament Spine Support cement (scale bar = 1 µm).rMSCs: rabbit mesenchymal stromal cells.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2 - License 3
Show All Figures
getmorefigures.php?uid=PMC3927750&req=5

fig6-2041731413509645: Scanning electron microscopy image showing rMSC (arrows) at day 1 of cell culture. The cell is covering micropore and is adherent onto the surface of the Cerament Spine Support cement (scale bar = 1 µm).rMSCs: rabbit mesenchymal stromal cells.
Mentions: A large number of rMSCs were adherent and were clearly visible on the surface of the Cerament Spine Support cement at all time points. At day 1, the rMSCs had started to adhere to the surface of the material and interact with the cement crystals (Figure 6). After day 3 of rMSC culture, there were areas where cells had proliferated well and aggregated with each other. Cells attained a typical size (60–160 µm in length), and they were polygonal or fusiform in shape (Figure 7). These cells had pseudopodia interacting with the crystals of the CS/HA cement (Figure 8). Cells showed accompanying filamentous fibres forming on the surface; this may be evidence for extracellular matrix production (Figure 8). After ascertaining that the surfaces supported rMSC growth, we moved to the three-dimensional (3D) model.

Bottom Line: The objective of this study was to investigate the visibility of loading of two types of commercially available cements, Cerament(™) Spine Support and Cerament Bone Void Filler with mesenchymal cells and cytokines (bone morphogenetic protein) to act as a biomimetic scaffolding for future clinical application.Determination of basic biocompatibility (cell viability) using methyl thiazolyl tetrazolium and live/dead assay was carried out using MG-63 cells at various time points.Results indicated that Cerament Spine Support was more biocompatible and that sequential injection of cement and then rabbit mesenchymal stromal cells into the tissue mimics is an optimal approach for clinical applications.

View Article: PubMed Central - PubMed

Affiliation: Glasgow Dental Hospital & School, University of Glasgow, Glasgow, UK.

ABSTRACT
Cements for maxillofacial reconstruction of jaw defects through calcification of rotated muscle have been tested. The objective of this study was to investigate the visibility of loading of two types of commercially available cements, Cerament(™) Spine Support and Cerament Bone Void Filler with mesenchymal cells and cytokines (bone morphogenetic protein) to act as a biomimetic scaffolding for future clinical application. Determination of basic biocompatibility (cell viability) using methyl thiazolyl tetrazolium and live/dead assay was carried out using MG-63 cells at various time points. Next, in order to inform potential subsequent in vivo experiments, a collagen tissue mimic was used for characterization of rabbit mesenchymal stromal cells using immunofluorescent cytoskeleton staining, and simultaneous and then sequential injection of Cerament Spine Support cement and cells into collagen gels. Results indicated that Cerament Spine Support was more biocompatible and that sequential injection of cement and then rabbit mesenchymal stromal cells into the tissue mimics is an optimal approach for clinical applications.

No MeSH data available.


Related in: MedlinePlus