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Assessment of cellular viability on calcium sulphate/hydroxyapatite injectable scaffolds.

Alfotawi R, Naudi K, Dalby MJ, Tanner KE, McMahon JD, Ayoub A - J Tissue Eng (2013)

Bottom Line: The objective of this study was to investigate the visibility of loading of two types of commercially available cements, Cerament(™) Spine Support and Cerament Bone Void Filler with mesenchymal cells and cytokines (bone morphogenetic protein) to act as a biomimetic scaffolding for future clinical application.Determination of basic biocompatibility (cell viability) using methyl thiazolyl tetrazolium and live/dead assay was carried out using MG-63 cells at various time points.Results indicated that Cerament Spine Support was more biocompatible and that sequential injection of cement and then rabbit mesenchymal stromal cells into the tissue mimics is an optimal approach for clinical applications.

View Article: PubMed Central - PubMed

Affiliation: Glasgow Dental Hospital & School, University of Glasgow, Glasgow, UK.

ABSTRACT
Cements for maxillofacial reconstruction of jaw defects through calcification of rotated muscle have been tested. The objective of this study was to investigate the visibility of loading of two types of commercially available cements, Cerament(™) Spine Support and Cerament Bone Void Filler with mesenchymal cells and cytokines (bone morphogenetic protein) to act as a biomimetic scaffolding for future clinical application. Determination of basic biocompatibility (cell viability) using methyl thiazolyl tetrazolium and live/dead assay was carried out using MG-63 cells at various time points. Next, in order to inform potential subsequent in vivo experiments, a collagen tissue mimic was used for characterization of rabbit mesenchymal stromal cells using immunofluorescent cytoskeleton staining, and simultaneous and then sequential injection of Cerament Spine Support cement and cells into collagen gels. Results indicated that Cerament Spine Support was more biocompatible and that sequential injection of cement and then rabbit mesenchymal stromal cells into the tissue mimics is an optimal approach for clinical applications.

No MeSH data available.


Related in: MedlinePlus

Photomicrographs for live and dead staining (calcein-AM and ethidium homodimer) for rMSCs in collagen after 24 h of cell culture: (a) presence of live rMSCs around the cement (Cerament Spine Support; green) after 24 h of cell culture (scale bare = 100 µm); (b) live cells inside a rat-tail collagen without cement (control) after 24 h of cell culture (scale bar = 50 µm). Result confirms presence of live cell evenly distributed around the injected cement.calcein-AM: acetomethoxy derivate of calcein; rMSCs: rabbit mesenchymal stromal cells.
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fig10-2041731413509645: Photomicrographs for live and dead staining (calcein-AM and ethidium homodimer) for rMSCs in collagen after 24 h of cell culture: (a) presence of live rMSCs around the cement (Cerament Spine Support; green) after 24 h of cell culture (scale bare = 100 µm); (b) live cells inside a rat-tail collagen without cement (control) after 24 h of cell culture (scale bar = 50 µm). Result confirms presence of live cell evenly distributed around the injected cement.calcein-AM: acetomethoxy derivate of calcein; rMSCs: rabbit mesenchymal stromal cells.

Mentions: In this collagen construct, many live cells were noted in the collagen gel with cell distribution when cell suspension was injected into the collagen between the cement, and live cells appeared green in colour (calcein-AM, Biolab), the percentage of live cells was 69.7% ± 12% after 24 h of cell seeding (Figure 10(a)). To provide a control for this, only experiment cells were injected into a collagen construct which showed even distribution of live cells inside collagen (Figure 10(b)). Understanding that sequential rather than parallel injection provided greater viability, we then investigated osteogenesis in vitro.


Assessment of cellular viability on calcium sulphate/hydroxyapatite injectable scaffolds.

Alfotawi R, Naudi K, Dalby MJ, Tanner KE, McMahon JD, Ayoub A - J Tissue Eng (2013)

Photomicrographs for live and dead staining (calcein-AM and ethidium homodimer) for rMSCs in collagen after 24 h of cell culture: (a) presence of live rMSCs around the cement (Cerament Spine Support; green) after 24 h of cell culture (scale bare = 100 µm); (b) live cells inside a rat-tail collagen without cement (control) after 24 h of cell culture (scale bar = 50 µm). Result confirms presence of live cell evenly distributed around the injected cement.calcein-AM: acetomethoxy derivate of calcein; rMSCs: rabbit mesenchymal stromal cells.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2 - License 3
Show All Figures
getmorefigures.php?uid=PMC3927750&req=5

fig10-2041731413509645: Photomicrographs for live and dead staining (calcein-AM and ethidium homodimer) for rMSCs in collagen after 24 h of cell culture: (a) presence of live rMSCs around the cement (Cerament Spine Support; green) after 24 h of cell culture (scale bare = 100 µm); (b) live cells inside a rat-tail collagen without cement (control) after 24 h of cell culture (scale bar = 50 µm). Result confirms presence of live cell evenly distributed around the injected cement.calcein-AM: acetomethoxy derivate of calcein; rMSCs: rabbit mesenchymal stromal cells.
Mentions: In this collagen construct, many live cells were noted in the collagen gel with cell distribution when cell suspension was injected into the collagen between the cement, and live cells appeared green in colour (calcein-AM, Biolab), the percentage of live cells was 69.7% ± 12% after 24 h of cell seeding (Figure 10(a)). To provide a control for this, only experiment cells were injected into a collagen construct which showed even distribution of live cells inside collagen (Figure 10(b)). Understanding that sequential rather than parallel injection provided greater viability, we then investigated osteogenesis in vitro.

Bottom Line: The objective of this study was to investigate the visibility of loading of two types of commercially available cements, Cerament(™) Spine Support and Cerament Bone Void Filler with mesenchymal cells and cytokines (bone morphogenetic protein) to act as a biomimetic scaffolding for future clinical application.Determination of basic biocompatibility (cell viability) using methyl thiazolyl tetrazolium and live/dead assay was carried out using MG-63 cells at various time points.Results indicated that Cerament Spine Support was more biocompatible and that sequential injection of cement and then rabbit mesenchymal stromal cells into the tissue mimics is an optimal approach for clinical applications.

View Article: PubMed Central - PubMed

Affiliation: Glasgow Dental Hospital & School, University of Glasgow, Glasgow, UK.

ABSTRACT
Cements for maxillofacial reconstruction of jaw defects through calcification of rotated muscle have been tested. The objective of this study was to investigate the visibility of loading of two types of commercially available cements, Cerament(™) Spine Support and Cerament Bone Void Filler with mesenchymal cells and cytokines (bone morphogenetic protein) to act as a biomimetic scaffolding for future clinical application. Determination of basic biocompatibility (cell viability) using methyl thiazolyl tetrazolium and live/dead assay was carried out using MG-63 cells at various time points. Next, in order to inform potential subsequent in vivo experiments, a collagen tissue mimic was used for characterization of rabbit mesenchymal stromal cells using immunofluorescent cytoskeleton staining, and simultaneous and then sequential injection of Cerament Spine Support cement and cells into collagen gels. Results indicated that Cerament Spine Support was more biocompatible and that sequential injection of cement and then rabbit mesenchymal stromal cells into the tissue mimics is an optimal approach for clinical applications.

No MeSH data available.


Related in: MedlinePlus