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Synthetic versus natural curcumin: bioequivalence in an in vitro oral mucositis model.

Lüer SC, Goette J, Troller R, Aebi C - BMC Complement Altern Med (2014)

Bottom Line: Its potent anti-inflammatory activity by inhibiting the activation of Nuclear Factor-κB is well established.The hypothesis was to demonstrate bioequivalence of nCUR and sCUR.The purity of sCUR was HPLC-confirmed.

View Article: PubMed Central - HTML - PubMed

Affiliation: Division of Pediatric Hematology/Oncology, Department of Pediatrics, University of Bern, Inselspital, Bern CH-3010, Switzerland. sonja.lueer@insel.ch.

ABSTRACT

Background: Curcumin (CUR) is a dietary spice and food colorant (E100). Its potent anti-inflammatory activity by inhibiting the activation of Nuclear Factor-κB is well established.

Methods: The aim of this study was to compare natural purified CUR (nCUR) with synthetically manufactured CUR (sCUR) with respect to their capacity to inhibit detrimental effects in an in vitro model of oral mucositis. The hypothesis was to demonstrate bioequivalence of nCUR and sCUR.

Results: The purity of sCUR was HPLC-confirmed. Adherence and invasion assays for bacteria to human pharyngeal epithelial cells demonstrated equivalence of nCUR and sCUR. Standard assays also demonstrated an identical inhibitory effect on pro-inflammatory cytokine/chemokine secretion (e.g., interleukin-8, interleukin-6) by Detroit pharyngeal cells exposed to bacterial stimuli. There was bioequivalence of sCUR and nCUR with respect to their antibacterial effects against various pharyngeal species.

Conclusion: nCUR and sCUR are equipotent in in vitro assays mimicking aspects of oral mucositis. The advantages of sCUR include that it is odorless and tasteless, more easily soluble in DMSO, and that it is a single, highly purified molecule, lacking the batch-to-batch variation of CUR content in nCUR. sCUR is a promising agent for the development of an oral anti-mucositis agent.

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Related in: MedlinePlus

Inhibition of release of pro-inflammatory cytokines by nCUR versus sCUR. Effect of nCUR and sCUR on secretion of GM-CSF, IL-6, IL-8, MCP-1, VEGF, and TNFα by Detroit cells preincubated for 0 or 60 minutes with 200 μM nCUR (green) or 200 μM sCUR (red) and subsequently infected for 4 hours with live M. catarrhalis strain 25238 (MOI 100). □ (black) indicates the positive control (0 μM CUR, MOI 100). At no time point there is a statistically significant difference between nCUR and sCUR.
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Figure 6: Inhibition of release of pro-inflammatory cytokines by nCUR versus sCUR. Effect of nCUR and sCUR on secretion of GM-CSF, IL-6, IL-8, MCP-1, VEGF, and TNFα by Detroit cells preincubated for 0 or 60 minutes with 200 μM nCUR (green) or 200 μM sCUR (red) and subsequently infected for 4 hours with live M. catarrhalis strain 25238 (MOI 100). □ (black) indicates the positive control (0 μM CUR, MOI 100). At no time point there is a statistically significant difference between nCUR and sCUR.

Mentions: As previously established, IL-8 was used as a representative of pro-inflammatory cytokines/chemokines[4,5]. Figure 5 demonstrates that both nCUR and sCUR similarly inhibit IL-8 secretion by Detroit cells (no statistical difference). This effect was seen for both concentrations of CUR tested. In addition, Luminex technology was used to assess the same cell supernatants for concentrations of GM-CSF, IL-6, IL-8, IL-10, MCP-1, VEGF, FGF-2, and TNFα. As expected[5], the release of all cytokines/chemokines with the exception of IL-10 and FGF-2 (not shown) was fully suppressed by pre-exposure to nCUR or sCUR for 60 minutes followed by stimulation of the monolayer with a bacterial MOI of 100 for 4 hours (Figure 6).


Synthetic versus natural curcumin: bioequivalence in an in vitro oral mucositis model.

Lüer SC, Goette J, Troller R, Aebi C - BMC Complement Altern Med (2014)

Inhibition of release of pro-inflammatory cytokines by nCUR versus sCUR. Effect of nCUR and sCUR on secretion of GM-CSF, IL-6, IL-8, MCP-1, VEGF, and TNFα by Detroit cells preincubated for 0 or 60 minutes with 200 μM nCUR (green) or 200 μM sCUR (red) and subsequently infected for 4 hours with live M. catarrhalis strain 25238 (MOI 100). □ (black) indicates the positive control (0 μM CUR, MOI 100). At no time point there is a statistically significant difference between nCUR and sCUR.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3927628&req=5

Figure 6: Inhibition of release of pro-inflammatory cytokines by nCUR versus sCUR. Effect of nCUR and sCUR on secretion of GM-CSF, IL-6, IL-8, MCP-1, VEGF, and TNFα by Detroit cells preincubated for 0 or 60 minutes with 200 μM nCUR (green) or 200 μM sCUR (red) and subsequently infected for 4 hours with live M. catarrhalis strain 25238 (MOI 100). □ (black) indicates the positive control (0 μM CUR, MOI 100). At no time point there is a statistically significant difference between nCUR and sCUR.
Mentions: As previously established, IL-8 was used as a representative of pro-inflammatory cytokines/chemokines[4,5]. Figure 5 demonstrates that both nCUR and sCUR similarly inhibit IL-8 secretion by Detroit cells (no statistical difference). This effect was seen for both concentrations of CUR tested. In addition, Luminex technology was used to assess the same cell supernatants for concentrations of GM-CSF, IL-6, IL-8, IL-10, MCP-1, VEGF, FGF-2, and TNFα. As expected[5], the release of all cytokines/chemokines with the exception of IL-10 and FGF-2 (not shown) was fully suppressed by pre-exposure to nCUR or sCUR for 60 minutes followed by stimulation of the monolayer with a bacterial MOI of 100 for 4 hours (Figure 6).

Bottom Line: Its potent anti-inflammatory activity by inhibiting the activation of Nuclear Factor-κB is well established.The hypothesis was to demonstrate bioequivalence of nCUR and sCUR.The purity of sCUR was HPLC-confirmed.

View Article: PubMed Central - HTML - PubMed

Affiliation: Division of Pediatric Hematology/Oncology, Department of Pediatrics, University of Bern, Inselspital, Bern CH-3010, Switzerland. sonja.lueer@insel.ch.

ABSTRACT

Background: Curcumin (CUR) is a dietary spice and food colorant (E100). Its potent anti-inflammatory activity by inhibiting the activation of Nuclear Factor-κB is well established.

Methods: The aim of this study was to compare natural purified CUR (nCUR) with synthetically manufactured CUR (sCUR) with respect to their capacity to inhibit detrimental effects in an in vitro model of oral mucositis. The hypothesis was to demonstrate bioequivalence of nCUR and sCUR.

Results: The purity of sCUR was HPLC-confirmed. Adherence and invasion assays for bacteria to human pharyngeal epithelial cells demonstrated equivalence of nCUR and sCUR. Standard assays also demonstrated an identical inhibitory effect on pro-inflammatory cytokine/chemokine secretion (e.g., interleukin-8, interleukin-6) by Detroit pharyngeal cells exposed to bacterial stimuli. There was bioequivalence of sCUR and nCUR with respect to their antibacterial effects against various pharyngeal species.

Conclusion: nCUR and sCUR are equipotent in in vitro assays mimicking aspects of oral mucositis. The advantages of sCUR include that it is odorless and tasteless, more easily soluble in DMSO, and that it is a single, highly purified molecule, lacking the batch-to-batch variation of CUR content in nCUR. sCUR is a promising agent for the development of an oral anti-mucositis agent.

Show MeSH
Related in: MedlinePlus