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Transauricular balloon angioplasty in rabbit thoracic aorta: a novel model of experimental restenosis.

Koniari I, Apostolakis E, Diamantopoulos A, Papadaki H, Papadimitriou E, Poimenidi E, Karnabatidis D, Karahaliou A, Costaridou L, Papalois A, Siablis D, Dougenis D, Alexopoulos D - Lipids Health Dis (2014)

Bottom Line: Group A compared to group B atherosclerotic lesions demonstrated similar percentage of macrophages (79.5 ± 9.56% versus 84 ± 12.2%; P = 0.869), more smooth muscle cells (61 ± 14.10% versus 40.5 ± 17.07; P = 0.027), increased intima/media ratio (1.20 ± 0.50 versus 0.62 ± 0.13; P = 0.015) despite the similar degree of intimal hyperplasia (9768 ± 1826.79 μm² versus 12205 ± 8789.23 μm²; P = 0.796), and further significant lumen deterioration (23722 ± 4508.11 versus 41967 ± 20344.61 μm²; P = 0.05) and total vessel area reduction (42350 ± 5819.70 versus 73190 ± 38902.79 μm²; P = 0.022).Group A and B animals revealed similar nitrated protein percentage (P = NS), but significantly higher protein nitration compared to control group (P < 0.01; P < 0.01, respectively).Transauricular balloon angioplasty constitutes a safe, minimally invasive and highly successful model of induced atherosclerosis in hyperlipidaemic rabbits.

View Article: PubMed Central - HTML - PubMed

Affiliation: Cardiothoracic Surgery Department, University Hospital of Patras, Rion Patras zip 25500, Greece. iokoniari@yahoo.gr.

ABSTRACT

Background: The aim of this study was to demonstrate a percutaneous transauricular method of balloon angioplasty in high-cholesterol fed rabbits, as an innovative atherosclerosis model.

Methods: Twenty male New Zealand rabbits were randomly divided into two groups of ten animals, as follows: atherogenic diet plus balloon angioplasty (group A) and atherogenic diet alone (group B). Balloon angioplasty was performed in the descending thoracic aorta through percutaneous catheterization of the auricular artery. Eight additional animals fed regular diet were served as long term control. At the end of 9 week period, rabbits were euthanized and thoracic aortas were isolated for histological, immunohistochemical and biochemical analysis.

Results: Atherogenic diet induced severe hypercholesterolemia in both group A and B (2802 ± 188.59 and 4423 ± 493.39 mg/dl respectively) compared to the control animals (55.5 ± 11.82 mg/dl; P < 0.001). Group A atherosclerotic lesions appeared to be more advanced histologically (20% type IV and 80% type V) compared to group B lesions (50% type III and 50% type IV). Group A compared to group B atherosclerotic lesions demonstrated similar percentage of macrophages (79.5 ± 9.56% versus 84 ± 12.2%; P = 0.869), more smooth muscle cells (61 ± 14.10% versus 40.5 ± 17.07; P = 0.027), increased intima/media ratio (1.20 ± 0.50 versus 0.62 ± 0.13; P = 0.015) despite the similar degree of intimal hyperplasia (9768 ± 1826.79 μm² versus 12205 ± 8789.23 μm²; P = 0.796), and further significant lumen deterioration (23722 ± 4508.11 versus 41967 ± 20344.61 μm²; P = 0.05) and total vessel area reduction (42350 ± 5819.70 versus 73190 ± 38902.79 μm²; P = 0.022). Group A and B animals revealed similar nitrated protein percentage (P = NS), but significantly higher protein nitration compared to control group (P < 0.01; P < 0.01, respectively). No deaths or systemic complications were reported.

Conclusion: Transauricular balloon angioplasty constitutes a safe, minimally invasive and highly successful model of induced atherosclerosis in hyperlipidaemic rabbits.

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Related in: MedlinePlus

Intraarterial transauricular access in the rabbit thoracic aorta. a. Direct percutaneous catheterization of the central auricular artery with a 22-gauge intravenous catheter, b. Repeated tract dilations of the peripheral transauricular artery with the introduction of a 4-F vascular sheath, c. Insertion of an 6.0-mm/40- mm (Cordis) peripheral dilatation balloon catheter over the guide wire, d-e Roadmap image of the auricular artery and the common carotid artery.
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Figure 6: Intraarterial transauricular access in the rabbit thoracic aorta. a. Direct percutaneous catheterization of the central auricular artery with a 22-gauge intravenous catheter, b. Repeated tract dilations of the peripheral transauricular artery with the introduction of a 4-F vascular sheath, c. Insertion of an 6.0-mm/40- mm (Cordis) peripheral dilatation balloon catheter over the guide wire, d-e Roadmap image of the auricular artery and the common carotid artery.

Mentions: The central auricular artery was the target vessel for cannulation and endovascular access of the descending thoracic aorta respectively. Initially, the auricular artery was punctured with a 22-gauge intravenous catheter (Helmflon; Helm Pharmaceuticals, Hamburg, Germany) approximately at the distal half of its subcutaneous course (Figure 6a). The needle of the catheter was removed, and 5 mL of contrast agent diluted with normal saline (1:1) was infused to obtain roadmap images of the extracranial carotid vasculature. Care was taken to aim at the distal half of the vessel, so as a second more proximal attempt might be performed in case of vasospasm or rupture [8].


Transauricular balloon angioplasty in rabbit thoracic aorta: a novel model of experimental restenosis.

Koniari I, Apostolakis E, Diamantopoulos A, Papadaki H, Papadimitriou E, Poimenidi E, Karnabatidis D, Karahaliou A, Costaridou L, Papalois A, Siablis D, Dougenis D, Alexopoulos D - Lipids Health Dis (2014)

Intraarterial transauricular access in the rabbit thoracic aorta. a. Direct percutaneous catheterization of the central auricular artery with a 22-gauge intravenous catheter, b. Repeated tract dilations of the peripheral transauricular artery with the introduction of a 4-F vascular sheath, c. Insertion of an 6.0-mm/40- mm (Cordis) peripheral dilatation balloon catheter over the guide wire, d-e Roadmap image of the auricular artery and the common carotid artery.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3927627&req=5

Figure 6: Intraarterial transauricular access in the rabbit thoracic aorta. a. Direct percutaneous catheterization of the central auricular artery with a 22-gauge intravenous catheter, b. Repeated tract dilations of the peripheral transauricular artery with the introduction of a 4-F vascular sheath, c. Insertion of an 6.0-mm/40- mm (Cordis) peripheral dilatation balloon catheter over the guide wire, d-e Roadmap image of the auricular artery and the common carotid artery.
Mentions: The central auricular artery was the target vessel for cannulation and endovascular access of the descending thoracic aorta respectively. Initially, the auricular artery was punctured with a 22-gauge intravenous catheter (Helmflon; Helm Pharmaceuticals, Hamburg, Germany) approximately at the distal half of its subcutaneous course (Figure 6a). The needle of the catheter was removed, and 5 mL of contrast agent diluted with normal saline (1:1) was infused to obtain roadmap images of the extracranial carotid vasculature. Care was taken to aim at the distal half of the vessel, so as a second more proximal attempt might be performed in case of vasospasm or rupture [8].

Bottom Line: Group A compared to group B atherosclerotic lesions demonstrated similar percentage of macrophages (79.5 ± 9.56% versus 84 ± 12.2%; P = 0.869), more smooth muscle cells (61 ± 14.10% versus 40.5 ± 17.07; P = 0.027), increased intima/media ratio (1.20 ± 0.50 versus 0.62 ± 0.13; P = 0.015) despite the similar degree of intimal hyperplasia (9768 ± 1826.79 μm² versus 12205 ± 8789.23 μm²; P = 0.796), and further significant lumen deterioration (23722 ± 4508.11 versus 41967 ± 20344.61 μm²; P = 0.05) and total vessel area reduction (42350 ± 5819.70 versus 73190 ± 38902.79 μm²; P = 0.022).Group A and B animals revealed similar nitrated protein percentage (P = NS), but significantly higher protein nitration compared to control group (P < 0.01; P < 0.01, respectively).Transauricular balloon angioplasty constitutes a safe, minimally invasive and highly successful model of induced atherosclerosis in hyperlipidaemic rabbits.

View Article: PubMed Central - HTML - PubMed

Affiliation: Cardiothoracic Surgery Department, University Hospital of Patras, Rion Patras zip 25500, Greece. iokoniari@yahoo.gr.

ABSTRACT

Background: The aim of this study was to demonstrate a percutaneous transauricular method of balloon angioplasty in high-cholesterol fed rabbits, as an innovative atherosclerosis model.

Methods: Twenty male New Zealand rabbits were randomly divided into two groups of ten animals, as follows: atherogenic diet plus balloon angioplasty (group A) and atherogenic diet alone (group B). Balloon angioplasty was performed in the descending thoracic aorta through percutaneous catheterization of the auricular artery. Eight additional animals fed regular diet were served as long term control. At the end of 9 week period, rabbits were euthanized and thoracic aortas were isolated for histological, immunohistochemical and biochemical analysis.

Results: Atherogenic diet induced severe hypercholesterolemia in both group A and B (2802 ± 188.59 and 4423 ± 493.39 mg/dl respectively) compared to the control animals (55.5 ± 11.82 mg/dl; P < 0.001). Group A atherosclerotic lesions appeared to be more advanced histologically (20% type IV and 80% type V) compared to group B lesions (50% type III and 50% type IV). Group A compared to group B atherosclerotic lesions demonstrated similar percentage of macrophages (79.5 ± 9.56% versus 84 ± 12.2%; P = 0.869), more smooth muscle cells (61 ± 14.10% versus 40.5 ± 17.07; P = 0.027), increased intima/media ratio (1.20 ± 0.50 versus 0.62 ± 0.13; P = 0.015) despite the similar degree of intimal hyperplasia (9768 ± 1826.79 μm² versus 12205 ± 8789.23 μm²; P = 0.796), and further significant lumen deterioration (23722 ± 4508.11 versus 41967 ± 20344.61 μm²; P = 0.05) and total vessel area reduction (42350 ± 5819.70 versus 73190 ± 38902.79 μm²; P = 0.022). Group A and B animals revealed similar nitrated protein percentage (P = NS), but significantly higher protein nitration compared to control group (P < 0.01; P < 0.01, respectively). No deaths or systemic complications were reported.

Conclusion: Transauricular balloon angioplasty constitutes a safe, minimally invasive and highly successful model of induced atherosclerosis in hyperlipidaemic rabbits.

Show MeSH
Related in: MedlinePlus