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Transauricular balloon angioplasty in rabbit thoracic aorta: a novel model of experimental restenosis.

Koniari I, Apostolakis E, Diamantopoulos A, Papadaki H, Papadimitriou E, Poimenidi E, Karnabatidis D, Karahaliou A, Costaridou L, Papalois A, Siablis D, Dougenis D, Alexopoulos D - Lipids Health Dis (2014)

Bottom Line: Group A compared to group B atherosclerotic lesions demonstrated similar percentage of macrophages (79.5 ± 9.56% versus 84 ± 12.2%; P = 0.869), more smooth muscle cells (61 ± 14.10% versus 40.5 ± 17.07; P = 0.027), increased intima/media ratio (1.20 ± 0.50 versus 0.62 ± 0.13; P = 0.015) despite the similar degree of intimal hyperplasia (9768 ± 1826.79 μm² versus 12205 ± 8789.23 μm²; P = 0.796), and further significant lumen deterioration (23722 ± 4508.11 versus 41967 ± 20344.61 μm²; P = 0.05) and total vessel area reduction (42350 ± 5819.70 versus 73190 ± 38902.79 μm²; P = 0.022).Group A and B animals revealed similar nitrated protein percentage (P = NS), but significantly higher protein nitration compared to control group (P < 0.01; P < 0.01, respectively).Transauricular balloon angioplasty constitutes a safe, minimally invasive and highly successful model of induced atherosclerosis in hyperlipidaemic rabbits.

View Article: PubMed Central - HTML - PubMed

Affiliation: Cardiothoracic Surgery Department, University Hospital of Patras, Rion Patras zip 25500, Greece. iokoniari@yahoo.gr.

ABSTRACT

Background: The aim of this study was to demonstrate a percutaneous transauricular method of balloon angioplasty in high-cholesterol fed rabbits, as an innovative atherosclerosis model.

Methods: Twenty male New Zealand rabbits were randomly divided into two groups of ten animals, as follows: atherogenic diet plus balloon angioplasty (group A) and atherogenic diet alone (group B). Balloon angioplasty was performed in the descending thoracic aorta through percutaneous catheterization of the auricular artery. Eight additional animals fed regular diet were served as long term control. At the end of 9 week period, rabbits were euthanized and thoracic aortas were isolated for histological, immunohistochemical and biochemical analysis.

Results: Atherogenic diet induced severe hypercholesterolemia in both group A and B (2802 ± 188.59 and 4423 ± 493.39 mg/dl respectively) compared to the control animals (55.5 ± 11.82 mg/dl; P < 0.001). Group A atherosclerotic lesions appeared to be more advanced histologically (20% type IV and 80% type V) compared to group B lesions (50% type III and 50% type IV). Group A compared to group B atherosclerotic lesions demonstrated similar percentage of macrophages (79.5 ± 9.56% versus 84 ± 12.2%; P = 0.869), more smooth muscle cells (61 ± 14.10% versus 40.5 ± 17.07; P = 0.027), increased intima/media ratio (1.20 ± 0.50 versus 0.62 ± 0.13; P = 0.015) despite the similar degree of intimal hyperplasia (9768 ± 1826.79 μm² versus 12205 ± 8789.23 μm²; P = 0.796), and further significant lumen deterioration (23722 ± 4508.11 versus 41967 ± 20344.61 μm²; P = 0.05) and total vessel area reduction (42350 ± 5819.70 versus 73190 ± 38902.79 μm²; P = 0.022). Group A and B animals revealed similar nitrated protein percentage (P = NS), but significantly higher protein nitration compared to control group (P < 0.01; P < 0.01, respectively). No deaths or systemic complications were reported.

Conclusion: Transauricular balloon angioplasty constitutes a safe, minimally invasive and highly successful model of induced atherosclerosis in hyperlipidaemic rabbits.

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Related in: MedlinePlus

Tyrosine nitration of proteins is not increased by the transauricular balloon angioplasty. a. Western Blot analysis for 3′-nitrotyrosine (upper panel) and β-actin (lower panel) in total protein lysates of descending aortas. b. The protein amounts were quantified by densitometric analysis of the corresponding bands and the ratio of nitrated proteins/β-actin was calculated for each lane. Results are expressed as mean ± S.E.M. of the percentage change of the amounts of 3′-nitrotyrosine compared with control animals. n.s, not statistically significant.
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Figure 5: Tyrosine nitration of proteins is not increased by the transauricular balloon angioplasty. a. Western Blot analysis for 3′-nitrotyrosine (upper panel) and β-actin (lower panel) in total protein lysates of descending aortas. b. The protein amounts were quantified by densitometric analysis of the corresponding bands and the ratio of nitrated proteins/β-actin was calculated for each lane. Results are expressed as mean ± S.E.M. of the percentage change of the amounts of 3′-nitrotyrosine compared with control animals. n.s, not statistically significant.

Mentions: The effect of the proposed injury model on protein tyrosine nitration was studied by measuring protein nitrotyrosine levels on aorta samples derived from control and animals fed with high cholesterol diet, injured and non-injured. High cholesterol diet significantly increased protein nitration levels, while transauricular ballon angioplasty did not cause any further increase (Figure 5).


Transauricular balloon angioplasty in rabbit thoracic aorta: a novel model of experimental restenosis.

Koniari I, Apostolakis E, Diamantopoulos A, Papadaki H, Papadimitriou E, Poimenidi E, Karnabatidis D, Karahaliou A, Costaridou L, Papalois A, Siablis D, Dougenis D, Alexopoulos D - Lipids Health Dis (2014)

Tyrosine nitration of proteins is not increased by the transauricular balloon angioplasty. a. Western Blot analysis for 3′-nitrotyrosine (upper panel) and β-actin (lower panel) in total protein lysates of descending aortas. b. The protein amounts were quantified by densitometric analysis of the corresponding bands and the ratio of nitrated proteins/β-actin was calculated for each lane. Results are expressed as mean ± S.E.M. of the percentage change of the amounts of 3′-nitrotyrosine compared with control animals. n.s, not statistically significant.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3927627&req=5

Figure 5: Tyrosine nitration of proteins is not increased by the transauricular balloon angioplasty. a. Western Blot analysis for 3′-nitrotyrosine (upper panel) and β-actin (lower panel) in total protein lysates of descending aortas. b. The protein amounts were quantified by densitometric analysis of the corresponding bands and the ratio of nitrated proteins/β-actin was calculated for each lane. Results are expressed as mean ± S.E.M. of the percentage change of the amounts of 3′-nitrotyrosine compared with control animals. n.s, not statistically significant.
Mentions: The effect of the proposed injury model on protein tyrosine nitration was studied by measuring protein nitrotyrosine levels on aorta samples derived from control and animals fed with high cholesterol diet, injured and non-injured. High cholesterol diet significantly increased protein nitration levels, while transauricular ballon angioplasty did not cause any further increase (Figure 5).

Bottom Line: Group A compared to group B atherosclerotic lesions demonstrated similar percentage of macrophages (79.5 ± 9.56% versus 84 ± 12.2%; P = 0.869), more smooth muscle cells (61 ± 14.10% versus 40.5 ± 17.07; P = 0.027), increased intima/media ratio (1.20 ± 0.50 versus 0.62 ± 0.13; P = 0.015) despite the similar degree of intimal hyperplasia (9768 ± 1826.79 μm² versus 12205 ± 8789.23 μm²; P = 0.796), and further significant lumen deterioration (23722 ± 4508.11 versus 41967 ± 20344.61 μm²; P = 0.05) and total vessel area reduction (42350 ± 5819.70 versus 73190 ± 38902.79 μm²; P = 0.022).Group A and B animals revealed similar nitrated protein percentage (P = NS), but significantly higher protein nitration compared to control group (P < 0.01; P < 0.01, respectively).Transauricular balloon angioplasty constitutes a safe, minimally invasive and highly successful model of induced atherosclerosis in hyperlipidaemic rabbits.

View Article: PubMed Central - HTML - PubMed

Affiliation: Cardiothoracic Surgery Department, University Hospital of Patras, Rion Patras zip 25500, Greece. iokoniari@yahoo.gr.

ABSTRACT

Background: The aim of this study was to demonstrate a percutaneous transauricular method of balloon angioplasty in high-cholesterol fed rabbits, as an innovative atherosclerosis model.

Methods: Twenty male New Zealand rabbits were randomly divided into two groups of ten animals, as follows: atherogenic diet plus balloon angioplasty (group A) and atherogenic diet alone (group B). Balloon angioplasty was performed in the descending thoracic aorta through percutaneous catheterization of the auricular artery. Eight additional animals fed regular diet were served as long term control. At the end of 9 week period, rabbits were euthanized and thoracic aortas were isolated for histological, immunohistochemical and biochemical analysis.

Results: Atherogenic diet induced severe hypercholesterolemia in both group A and B (2802 ± 188.59 and 4423 ± 493.39 mg/dl respectively) compared to the control animals (55.5 ± 11.82 mg/dl; P < 0.001). Group A atherosclerotic lesions appeared to be more advanced histologically (20% type IV and 80% type V) compared to group B lesions (50% type III and 50% type IV). Group A compared to group B atherosclerotic lesions demonstrated similar percentage of macrophages (79.5 ± 9.56% versus 84 ± 12.2%; P = 0.869), more smooth muscle cells (61 ± 14.10% versus 40.5 ± 17.07; P = 0.027), increased intima/media ratio (1.20 ± 0.50 versus 0.62 ± 0.13; P = 0.015) despite the similar degree of intimal hyperplasia (9768 ± 1826.79 μm² versus 12205 ± 8789.23 μm²; P = 0.796), and further significant lumen deterioration (23722 ± 4508.11 versus 41967 ± 20344.61 μm²; P = 0.05) and total vessel area reduction (42350 ± 5819.70 versus 73190 ± 38902.79 μm²; P = 0.022). Group A and B animals revealed similar nitrated protein percentage (P = NS), but significantly higher protein nitration compared to control group (P < 0.01; P < 0.01, respectively). No deaths or systemic complications were reported.

Conclusion: Transauricular balloon angioplasty constitutes a safe, minimally invasive and highly successful model of induced atherosclerosis in hyperlipidaemic rabbits.

Show MeSH
Related in: MedlinePlus