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Transauricular balloon angioplasty in rabbit thoracic aorta: a novel model of experimental restenosis.

Koniari I, Apostolakis E, Diamantopoulos A, Papadaki H, Papadimitriou E, Poimenidi E, Karnabatidis D, Karahaliou A, Costaridou L, Papalois A, Siablis D, Dougenis D, Alexopoulos D - Lipids Health Dis (2014)

Bottom Line: Group A compared to group B atherosclerotic lesions demonstrated similar percentage of macrophages (79.5 ± 9.56% versus 84 ± 12.2%; P = 0.869), more smooth muscle cells (61 ± 14.10% versus 40.5 ± 17.07; P = 0.027), increased intima/media ratio (1.20 ± 0.50 versus 0.62 ± 0.13; P = 0.015) despite the similar degree of intimal hyperplasia (9768 ± 1826.79 μm² versus 12205 ± 8789.23 μm²; P = 0.796), and further significant lumen deterioration (23722 ± 4508.11 versus 41967 ± 20344.61 μm²; P = 0.05) and total vessel area reduction (42350 ± 5819.70 versus 73190 ± 38902.79 μm²; P = 0.022).Group A and B animals revealed similar nitrated protein percentage (P = NS), but significantly higher protein nitration compared to control group (P < 0.01; P < 0.01, respectively).Transauricular balloon angioplasty constitutes a safe, minimally invasive and highly successful model of induced atherosclerosis in hyperlipidaemic rabbits.

View Article: PubMed Central - HTML - PubMed

Affiliation: Cardiothoracic Surgery Department, University Hospital of Patras, Rion Patras zip 25500, Greece. iokoniari@yahoo.gr.

ABSTRACT

Background: The aim of this study was to demonstrate a percutaneous transauricular method of balloon angioplasty in high-cholesterol fed rabbits, as an innovative atherosclerosis model.

Methods: Twenty male New Zealand rabbits were randomly divided into two groups of ten animals, as follows: atherogenic diet plus balloon angioplasty (group A) and atherogenic diet alone (group B). Balloon angioplasty was performed in the descending thoracic aorta through percutaneous catheterization of the auricular artery. Eight additional animals fed regular diet were served as long term control. At the end of 9 week period, rabbits were euthanized and thoracic aortas were isolated for histological, immunohistochemical and biochemical analysis.

Results: Atherogenic diet induced severe hypercholesterolemia in both group A and B (2802 ± 188.59 and 4423 ± 493.39 mg/dl respectively) compared to the control animals (55.5 ± 11.82 mg/dl; P < 0.001). Group A atherosclerotic lesions appeared to be more advanced histologically (20% type IV and 80% type V) compared to group B lesions (50% type III and 50% type IV). Group A compared to group B atherosclerotic lesions demonstrated similar percentage of macrophages (79.5 ± 9.56% versus 84 ± 12.2%; P = 0.869), more smooth muscle cells (61 ± 14.10% versus 40.5 ± 17.07; P = 0.027), increased intima/media ratio (1.20 ± 0.50 versus 0.62 ± 0.13; P = 0.015) despite the similar degree of intimal hyperplasia (9768 ± 1826.79 μm² versus 12205 ± 8789.23 μm²; P = 0.796), and further significant lumen deterioration (23722 ± 4508.11 versus 41967 ± 20344.61 μm²; P = 0.05) and total vessel area reduction (42350 ± 5819.70 versus 73190 ± 38902.79 μm²; P = 0.022). Group A and B animals revealed similar nitrated protein percentage (P = NS), but significantly higher protein nitration compared to control group (P < 0.01; P < 0.01, respectively). No deaths or systemic complications were reported.

Conclusion: Transauricular balloon angioplasty constitutes a safe, minimally invasive and highly successful model of induced atherosclerosis in hyperlipidaemic rabbits.

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Related in: MedlinePlus

Ram-11 and HHF-35 staining in thoracic aortas. a1. Plethora RAM-11 positive foam cells, and a2. significant amounts of positive for a-actin SMCs are observed in angioplasty induced atherosclerotic lesions. b1. Significant foam cell formation, and b2. several a-actin positive SMCs between foam cells in spontaneous atherosclerotic lesions. c1. Lack of foam cell deposition, and c2. Normal presence of HHF-35 positive SMCs in the media of control aortas.
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Figure 3: Ram-11 and HHF-35 staining in thoracic aortas. a1. Plethora RAM-11 positive foam cells, and a2. significant amounts of positive for a-actin SMCs are observed in angioplasty induced atherosclerotic lesions. b1. Significant foam cell formation, and b2. several a-actin positive SMCs between foam cells in spontaneous atherosclerotic lesions. c1. Lack of foam cell deposition, and c2. Normal presence of HHF-35 positive SMCs in the media of control aortas.

Mentions: The atherogenic diet was associated with a significant increase in lipid deposition and foam cell formation, indicated by the increase in RAM-11 immunoreactivity in group A and B animals. The aortas of group A rabbits were mainly strongly positive (n = 8 animals) for RAM-11 staining, except two cases that were moderately positive. Similarly, almost all the aortas of group B rabbits revealed strongly positive (n = 9 animals) RAM-11 immunoreactivity. It is prominent that the percentage of RAM-11 positive cells had no significant difference between spontaneous and angioplasty- induced atherosclerotic lesions (79.5 ± 9.56% versus 84 ± 12,2% respectively; P = 0.869), as shown in Figure 2. Also, the relative number of macrophages was associated with the severity of atherosclerotic lesions, as demonstrated in Figure 3a1 and 3b1.


Transauricular balloon angioplasty in rabbit thoracic aorta: a novel model of experimental restenosis.

Koniari I, Apostolakis E, Diamantopoulos A, Papadaki H, Papadimitriou E, Poimenidi E, Karnabatidis D, Karahaliou A, Costaridou L, Papalois A, Siablis D, Dougenis D, Alexopoulos D - Lipids Health Dis (2014)

Ram-11 and HHF-35 staining in thoracic aortas. a1. Plethora RAM-11 positive foam cells, and a2. significant amounts of positive for a-actin SMCs are observed in angioplasty induced atherosclerotic lesions. b1. Significant foam cell formation, and b2. several a-actin positive SMCs between foam cells in spontaneous atherosclerotic lesions. c1. Lack of foam cell deposition, and c2. Normal presence of HHF-35 positive SMCs in the media of control aortas.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3927627&req=5

Figure 3: Ram-11 and HHF-35 staining in thoracic aortas. a1. Plethora RAM-11 positive foam cells, and a2. significant amounts of positive for a-actin SMCs are observed in angioplasty induced atherosclerotic lesions. b1. Significant foam cell formation, and b2. several a-actin positive SMCs between foam cells in spontaneous atherosclerotic lesions. c1. Lack of foam cell deposition, and c2. Normal presence of HHF-35 positive SMCs in the media of control aortas.
Mentions: The atherogenic diet was associated with a significant increase in lipid deposition and foam cell formation, indicated by the increase in RAM-11 immunoreactivity in group A and B animals. The aortas of group A rabbits were mainly strongly positive (n = 8 animals) for RAM-11 staining, except two cases that were moderately positive. Similarly, almost all the aortas of group B rabbits revealed strongly positive (n = 9 animals) RAM-11 immunoreactivity. It is prominent that the percentage of RAM-11 positive cells had no significant difference between spontaneous and angioplasty- induced atherosclerotic lesions (79.5 ± 9.56% versus 84 ± 12,2% respectively; P = 0.869), as shown in Figure 2. Also, the relative number of macrophages was associated with the severity of atherosclerotic lesions, as demonstrated in Figure 3a1 and 3b1.

Bottom Line: Group A compared to group B atherosclerotic lesions demonstrated similar percentage of macrophages (79.5 ± 9.56% versus 84 ± 12.2%; P = 0.869), more smooth muscle cells (61 ± 14.10% versus 40.5 ± 17.07; P = 0.027), increased intima/media ratio (1.20 ± 0.50 versus 0.62 ± 0.13; P = 0.015) despite the similar degree of intimal hyperplasia (9768 ± 1826.79 μm² versus 12205 ± 8789.23 μm²; P = 0.796), and further significant lumen deterioration (23722 ± 4508.11 versus 41967 ± 20344.61 μm²; P = 0.05) and total vessel area reduction (42350 ± 5819.70 versus 73190 ± 38902.79 μm²; P = 0.022).Group A and B animals revealed similar nitrated protein percentage (P = NS), but significantly higher protein nitration compared to control group (P < 0.01; P < 0.01, respectively).Transauricular balloon angioplasty constitutes a safe, minimally invasive and highly successful model of induced atherosclerosis in hyperlipidaemic rabbits.

View Article: PubMed Central - HTML - PubMed

Affiliation: Cardiothoracic Surgery Department, University Hospital of Patras, Rion Patras zip 25500, Greece. iokoniari@yahoo.gr.

ABSTRACT

Background: The aim of this study was to demonstrate a percutaneous transauricular method of balloon angioplasty in high-cholesterol fed rabbits, as an innovative atherosclerosis model.

Methods: Twenty male New Zealand rabbits were randomly divided into two groups of ten animals, as follows: atherogenic diet plus balloon angioplasty (group A) and atherogenic diet alone (group B). Balloon angioplasty was performed in the descending thoracic aorta through percutaneous catheterization of the auricular artery. Eight additional animals fed regular diet were served as long term control. At the end of 9 week period, rabbits were euthanized and thoracic aortas were isolated for histological, immunohistochemical and biochemical analysis.

Results: Atherogenic diet induced severe hypercholesterolemia in both group A and B (2802 ± 188.59 and 4423 ± 493.39 mg/dl respectively) compared to the control animals (55.5 ± 11.82 mg/dl; P < 0.001). Group A atherosclerotic lesions appeared to be more advanced histologically (20% type IV and 80% type V) compared to group B lesions (50% type III and 50% type IV). Group A compared to group B atherosclerotic lesions demonstrated similar percentage of macrophages (79.5 ± 9.56% versus 84 ± 12.2%; P = 0.869), more smooth muscle cells (61 ± 14.10% versus 40.5 ± 17.07; P = 0.027), increased intima/media ratio (1.20 ± 0.50 versus 0.62 ± 0.13; P = 0.015) despite the similar degree of intimal hyperplasia (9768 ± 1826.79 μm² versus 12205 ± 8789.23 μm²; P = 0.796), and further significant lumen deterioration (23722 ± 4508.11 versus 41967 ± 20344.61 μm²; P = 0.05) and total vessel area reduction (42350 ± 5819.70 versus 73190 ± 38902.79 μm²; P = 0.022). Group A and B animals revealed similar nitrated protein percentage (P = NS), but significantly higher protein nitration compared to control group (P < 0.01; P < 0.01, respectively). No deaths or systemic complications were reported.

Conclusion: Transauricular balloon angioplasty constitutes a safe, minimally invasive and highly successful model of induced atherosclerosis in hyperlipidaemic rabbits.

Show MeSH
Related in: MedlinePlus