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Eicosapentaenoic acid/docosahexaenoic acid 1:1 ratio improves histological alterations in obese rats with metabolic syndrome.

Taltavull N, Muñoz-Cortés M, Lluís L, Jové M, Fortuño A, Molinar-Toribio E, Torres JL, Pazos M, Medina I, Nogués MR - Lipids Health Dis (2014)

Bottom Line: The quantitative data were expressed by mean ± SD and were compared among groups and treatments using ANOVA with post-hoc tests for parametric data and the U-Mann-Whitney for non-parametric data.Qualitative data were expressed in frequencies, and compared with contingency tables using χ² statistics.In both strains EPA:DHA 1:1 treatment improved inflammation related parameters in liver and kidney.

View Article: PubMed Central - HTML - PubMed

Affiliation: Unit of Pharmacology, Faculty of Medicine and Health Sciences, Rovira i Virgili University, Reus, Spain. nuria.taltavull@urv.cat.

ABSTRACT

Background: Marine polyunsaturated fatty acids, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) have been associated with improvement in the Metabolic Syndrome (MS). The aim of this study is to evaluate how three fish-oil diets with different eicosapentaenoic acid/docosahexaenoic acid ratios (EPA/DHA ratio) affect the histology of liver, kidney, adipose tissue and aorta in a preliminary morphological study. This work uses an animal model of metabolic syndrome in comparison with healthy animals in order to provide information about the best EPA:DHA ratio to prevent or to improve metabolic syndrome symptoms.

Methods: 35 Wistar rats, as a control, and 35 spontaneously hypertensive obese rats (SHROB) were fed for 13 weeks with 3 different supplementation of fish oil containing EPA and DHA ratios (1:1, 2:1 and 1:2, respectively). All samples were stained with haematoxylin/eosin stain, except aorta samples, which were stained also with Verhoeff and van Gieson's stain. A histological study was carried out to evaluate changes. These changes were statistically analyzed using SPSS IBM 19 software. The quantitative data were expressed by mean ± SD and were compared among groups and treatments using ANOVA with post-hoc tests for parametric data and the U-Mann-Whitney for non-parametric data. Qualitative data were expressed in frequencies, and compared with contingency tables using χ² statistics.

Results: EPA:DHA 1:1 treatment tended to improve the density and the wrinkling of elastic layers in SHROB rats. Only Wistar rats fed with EPA:DHA 1:1 treatment did not show mast cells in adipose tissue and has less kidney atrophy. In both strains EPA:DHA 1:1 treatment improved inflammation related parameters in liver and kidney.

Conclusions: EPA:DHA 1:1 treatment was the most beneficial treatment since improved many histological parameters in both groups of rats.

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Kidney results. At the top Histological cuts of kidney, Hematoxilin-eosin stain. (A) EPA:DHA 1:1 in SHROB rat (40x) thyroidization zones are marked by black arrows. Thyroidization it’s a kind of atrophy which gives them the appearance of the follicles in the thyroid gland as we can see in the picture (hence the name). (B) EPA:DHA 1:2 in SHROB rat (200x), inflammation in kidney can be observed by the presence of inflammatory cells, also we can observe a normal glomerulus on the top and a glomerulus with glomerulosclerosis marked by a black arrow. At the bottom three graphics in which: (W) refers to Wistar strain, (S) refers to SHROB strain. (A) EPA:DHA 1:1, (B) EPA:DHA 2:1, (C) EPA:DHA 1:2. Equal letters, a, b, c, d, e means statistical differences among strains. (C) We found greater presence of glomerulosclerosis in SHROB rats than in Wistar rats, but significant differences were found only in the EPA:DHA 1:1 treatment (p = 0.005a). (D) SHROB rats had greater thyroidization than Wistar rats in all treatment groups p = 0.003a, p = 0.032b, p = 0.005c. (E) Atrophy was also higher in SHROB rats. EPA:1:1 doesn’t shown atrophy in either both treatments, but no significant differences were founded. (F) Inflammation were better also in EPA:DHA 1:1 treatments in both strains, but without significant differences.
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Figure 6: Kidney results. At the top Histological cuts of kidney, Hematoxilin-eosin stain. (A) EPA:DHA 1:1 in SHROB rat (40x) thyroidization zones are marked by black arrows. Thyroidization it’s a kind of atrophy which gives them the appearance of the follicles in the thyroid gland as we can see in the picture (hence the name). (B) EPA:DHA 1:2 in SHROB rat (200x), inflammation in kidney can be observed by the presence of inflammatory cells, also we can observe a normal glomerulus on the top and a glomerulus with glomerulosclerosis marked by a black arrow. At the bottom three graphics in which: (W) refers to Wistar strain, (S) refers to SHROB strain. (A) EPA:DHA 1:1, (B) EPA:DHA 2:1, (C) EPA:DHA 1:2. Equal letters, a, b, c, d, e means statistical differences among strains. (C) We found greater presence of glomerulosclerosis in SHROB rats than in Wistar rats, but significant differences were found only in the EPA:DHA 1:1 treatment (p = 0.005a). (D) SHROB rats had greater thyroidization than Wistar rats in all treatment groups p = 0.003a, p = 0.032b, p = 0.005c. (E) Atrophy was also higher in SHROB rats. EPA:1:1 doesn’t shown atrophy in either both treatments, but no significant differences were founded. (F) Inflammation were better also in EPA:DHA 1:1 treatments in both strains, but without significant differences.

Mentions: Table 3 and Figure 6 shows the results of the different variables studied in kidney. To sum up, kidney damage was greater in SHROB than in Wistar rats. The 2:1 treatment tends to worsen the kidney parameters in Wistar rats, while the 1:1 treatment improves renal atrophy in SHROB in comparison to the other tretments. The EPA:DHA 1:1 treatment had the lowest presence of atrophy followed by EPA:DHA 1:2. There were no differences in fibrosis, lipid depositions or inflammation of the kidney either between treatments or between strains.


Eicosapentaenoic acid/docosahexaenoic acid 1:1 ratio improves histological alterations in obese rats with metabolic syndrome.

Taltavull N, Muñoz-Cortés M, Lluís L, Jové M, Fortuño A, Molinar-Toribio E, Torres JL, Pazos M, Medina I, Nogués MR - Lipids Health Dis (2014)

Kidney results. At the top Histological cuts of kidney, Hematoxilin-eosin stain. (A) EPA:DHA 1:1 in SHROB rat (40x) thyroidization zones are marked by black arrows. Thyroidization it’s a kind of atrophy which gives them the appearance of the follicles in the thyroid gland as we can see in the picture (hence the name). (B) EPA:DHA 1:2 in SHROB rat (200x), inflammation in kidney can be observed by the presence of inflammatory cells, also we can observe a normal glomerulus on the top and a glomerulus with glomerulosclerosis marked by a black arrow. At the bottom three graphics in which: (W) refers to Wistar strain, (S) refers to SHROB strain. (A) EPA:DHA 1:1, (B) EPA:DHA 2:1, (C) EPA:DHA 1:2. Equal letters, a, b, c, d, e means statistical differences among strains. (C) We found greater presence of glomerulosclerosis in SHROB rats than in Wistar rats, but significant differences were found only in the EPA:DHA 1:1 treatment (p = 0.005a). (D) SHROB rats had greater thyroidization than Wistar rats in all treatment groups p = 0.003a, p = 0.032b, p = 0.005c. (E) Atrophy was also higher in SHROB rats. EPA:1:1 doesn’t shown atrophy in either both treatments, but no significant differences were founded. (F) Inflammation were better also in EPA:DHA 1:1 treatments in both strains, but without significant differences.
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Related In: Results  -  Collection

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Figure 6: Kidney results. At the top Histological cuts of kidney, Hematoxilin-eosin stain. (A) EPA:DHA 1:1 in SHROB rat (40x) thyroidization zones are marked by black arrows. Thyroidization it’s a kind of atrophy which gives them the appearance of the follicles in the thyroid gland as we can see in the picture (hence the name). (B) EPA:DHA 1:2 in SHROB rat (200x), inflammation in kidney can be observed by the presence of inflammatory cells, also we can observe a normal glomerulus on the top and a glomerulus with glomerulosclerosis marked by a black arrow. At the bottom three graphics in which: (W) refers to Wistar strain, (S) refers to SHROB strain. (A) EPA:DHA 1:1, (B) EPA:DHA 2:1, (C) EPA:DHA 1:2. Equal letters, a, b, c, d, e means statistical differences among strains. (C) We found greater presence of glomerulosclerosis in SHROB rats than in Wistar rats, but significant differences were found only in the EPA:DHA 1:1 treatment (p = 0.005a). (D) SHROB rats had greater thyroidization than Wistar rats in all treatment groups p = 0.003a, p = 0.032b, p = 0.005c. (E) Atrophy was also higher in SHROB rats. EPA:1:1 doesn’t shown atrophy in either both treatments, but no significant differences were founded. (F) Inflammation were better also in EPA:DHA 1:1 treatments in both strains, but without significant differences.
Mentions: Table 3 and Figure 6 shows the results of the different variables studied in kidney. To sum up, kidney damage was greater in SHROB than in Wistar rats. The 2:1 treatment tends to worsen the kidney parameters in Wistar rats, while the 1:1 treatment improves renal atrophy in SHROB in comparison to the other tretments. The EPA:DHA 1:1 treatment had the lowest presence of atrophy followed by EPA:DHA 1:2. There were no differences in fibrosis, lipid depositions or inflammation of the kidney either between treatments or between strains.

Bottom Line: The quantitative data were expressed by mean ± SD and were compared among groups and treatments using ANOVA with post-hoc tests for parametric data and the U-Mann-Whitney for non-parametric data.Qualitative data were expressed in frequencies, and compared with contingency tables using χ² statistics.In both strains EPA:DHA 1:1 treatment improved inflammation related parameters in liver and kidney.

View Article: PubMed Central - HTML - PubMed

Affiliation: Unit of Pharmacology, Faculty of Medicine and Health Sciences, Rovira i Virgili University, Reus, Spain. nuria.taltavull@urv.cat.

ABSTRACT

Background: Marine polyunsaturated fatty acids, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) have been associated with improvement in the Metabolic Syndrome (MS). The aim of this study is to evaluate how three fish-oil diets with different eicosapentaenoic acid/docosahexaenoic acid ratios (EPA/DHA ratio) affect the histology of liver, kidney, adipose tissue and aorta in a preliminary morphological study. This work uses an animal model of metabolic syndrome in comparison with healthy animals in order to provide information about the best EPA:DHA ratio to prevent or to improve metabolic syndrome symptoms.

Methods: 35 Wistar rats, as a control, and 35 spontaneously hypertensive obese rats (SHROB) were fed for 13 weeks with 3 different supplementation of fish oil containing EPA and DHA ratios (1:1, 2:1 and 1:2, respectively). All samples were stained with haematoxylin/eosin stain, except aorta samples, which were stained also with Verhoeff and van Gieson's stain. A histological study was carried out to evaluate changes. These changes were statistically analyzed using SPSS IBM 19 software. The quantitative data were expressed by mean ± SD and were compared among groups and treatments using ANOVA with post-hoc tests for parametric data and the U-Mann-Whitney for non-parametric data. Qualitative data were expressed in frequencies, and compared with contingency tables using χ² statistics.

Results: EPA:DHA 1:1 treatment tended to improve the density and the wrinkling of elastic layers in SHROB rats. Only Wistar rats fed with EPA:DHA 1:1 treatment did not show mast cells in adipose tissue and has less kidney atrophy. In both strains EPA:DHA 1:1 treatment improved inflammation related parameters in liver and kidney.

Conclusions: EPA:DHA 1:1 treatment was the most beneficial treatment since improved many histological parameters in both groups of rats.

Show MeSH
Related in: MedlinePlus