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Eicosapentaenoic acid/docosahexaenoic acid 1:1 ratio improves histological alterations in obese rats with metabolic syndrome.

Taltavull N, Muñoz-Cortés M, Lluís L, Jové M, Fortuño A, Molinar-Toribio E, Torres JL, Pazos M, Medina I, Nogués MR - Lipids Health Dis (2014)

Bottom Line: The quantitative data were expressed by mean ± SD and were compared among groups and treatments using ANOVA with post-hoc tests for parametric data and the U-Mann-Whitney for non-parametric data.Qualitative data were expressed in frequencies, and compared with contingency tables using χ² statistics.In both strains EPA:DHA 1:1 treatment improved inflammation related parameters in liver and kidney.

View Article: PubMed Central - HTML - PubMed

Affiliation: Unit of Pharmacology, Faculty of Medicine and Health Sciences, Rovira i Virgili University, Reus, Spain. nuria.taltavull@urv.cat.

ABSTRACT

Background: Marine polyunsaturated fatty acids, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) have been associated with improvement in the Metabolic Syndrome (MS). The aim of this study is to evaluate how three fish-oil diets with different eicosapentaenoic acid/docosahexaenoic acid ratios (EPA/DHA ratio) affect the histology of liver, kidney, adipose tissue and aorta in a preliminary morphological study. This work uses an animal model of metabolic syndrome in comparison with healthy animals in order to provide information about the best EPA:DHA ratio to prevent or to improve metabolic syndrome symptoms.

Methods: 35 Wistar rats, as a control, and 35 spontaneously hypertensive obese rats (SHROB) were fed for 13 weeks with 3 different supplementation of fish oil containing EPA and DHA ratios (1:1, 2:1 and 1:2, respectively). All samples were stained with haematoxylin/eosin stain, except aorta samples, which were stained also with Verhoeff and van Gieson's stain. A histological study was carried out to evaluate changes. These changes were statistically analyzed using SPSS IBM 19 software. The quantitative data were expressed by mean ± SD and were compared among groups and treatments using ANOVA with post-hoc tests for parametric data and the U-Mann-Whitney for non-parametric data. Qualitative data were expressed in frequencies, and compared with contingency tables using χ² statistics.

Results: EPA:DHA 1:1 treatment tended to improve the density and the wrinkling of elastic layers in SHROB rats. Only Wistar rats fed with EPA:DHA 1:1 treatment did not show mast cells in adipose tissue and has less kidney atrophy. In both strains EPA:DHA 1:1 treatment improved inflammation related parameters in liver and kidney.

Conclusions: EPA:DHA 1:1 treatment was the most beneficial treatment since improved many histological parameters in both groups of rats.

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Related in: MedlinePlus

Histological cuts of liver. Histological cuts of liver, Hematoxilin-eosin stain (A) EPA:DHA 2:1 treatment in SHROB rat (40x), shown steatosis with periportal location (B) EPA:DHA 2:1 treatment in SHROB rat (400x), lobular inflammation marked up with a black arrow. The picture also allows us to observe steatosi which is marked up, in part, with red arrows (C) EPA:DHA 2:1 in SHROB rat (600x), on the right some macrophages surrounding a lipidic drop, forming a lipogrnaulome and on the left a lipogranulome also formed by macrophages. The picture also allows us to observe steatosi at higher magnification (D) EPA:DHA 2:1 in Wistar rat (200x), portal inflammation, as we can see the inflammatory cells are surrounding portal space.
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Figure 5: Histological cuts of liver. Histological cuts of liver, Hematoxilin-eosin stain (A) EPA:DHA 2:1 treatment in SHROB rat (40x), shown steatosis with periportal location (B) EPA:DHA 2:1 treatment in SHROB rat (400x), lobular inflammation marked up with a black arrow. The picture also allows us to observe steatosi which is marked up, in part, with red arrows (C) EPA:DHA 2:1 in SHROB rat (600x), on the right some macrophages surrounding a lipidic drop, forming a lipogrnaulome and on the left a lipogranulome also formed by macrophages. The picture also allows us to observe steatosi at higher magnification (D) EPA:DHA 2:1 in Wistar rat (200x), portal inflammation, as we can see the inflammatory cells are surrounding portal space.

Mentions: Results are shown in Figures 4 and 5. To sum up, in liver, the presence of steatosis in the centrilobular zone indicates initial harmful effects. The more widespread it is, the more the injury has progressed and the more severe it is. Therefore, periportal steatosis is worse than centrilobular steatosis, and non-zonal inflammation is the worst. In SHROB rats steatosis tended to be periportal, but no statistically significant differences were found between treatment groups. As in the other tissues, inflammation was evaluated according to the presence of white cells. SHROB rats also showed greater lobular inflammation than Wistar.


Eicosapentaenoic acid/docosahexaenoic acid 1:1 ratio improves histological alterations in obese rats with metabolic syndrome.

Taltavull N, Muñoz-Cortés M, Lluís L, Jové M, Fortuño A, Molinar-Toribio E, Torres JL, Pazos M, Medina I, Nogués MR - Lipids Health Dis (2014)

Histological cuts of liver. Histological cuts of liver, Hematoxilin-eosin stain (A) EPA:DHA 2:1 treatment in SHROB rat (40x), shown steatosis with periportal location (B) EPA:DHA 2:1 treatment in SHROB rat (400x), lobular inflammation marked up with a black arrow. The picture also allows us to observe steatosi which is marked up, in part, with red arrows (C) EPA:DHA 2:1 in SHROB rat (600x), on the right some macrophages surrounding a lipidic drop, forming a lipogrnaulome and on the left a lipogranulome also formed by macrophages. The picture also allows us to observe steatosi at higher magnification (D) EPA:DHA 2:1 in Wistar rat (200x), portal inflammation, as we can see the inflammatory cells are surrounding portal space.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3927584&req=5

Figure 5: Histological cuts of liver. Histological cuts of liver, Hematoxilin-eosin stain (A) EPA:DHA 2:1 treatment in SHROB rat (40x), shown steatosis with periportal location (B) EPA:DHA 2:1 treatment in SHROB rat (400x), lobular inflammation marked up with a black arrow. The picture also allows us to observe steatosi which is marked up, in part, with red arrows (C) EPA:DHA 2:1 in SHROB rat (600x), on the right some macrophages surrounding a lipidic drop, forming a lipogrnaulome and on the left a lipogranulome also formed by macrophages. The picture also allows us to observe steatosi at higher magnification (D) EPA:DHA 2:1 in Wistar rat (200x), portal inflammation, as we can see the inflammatory cells are surrounding portal space.
Mentions: Results are shown in Figures 4 and 5. To sum up, in liver, the presence of steatosis in the centrilobular zone indicates initial harmful effects. The more widespread it is, the more the injury has progressed and the more severe it is. Therefore, periportal steatosis is worse than centrilobular steatosis, and non-zonal inflammation is the worst. In SHROB rats steatosis tended to be periportal, but no statistically significant differences were found between treatment groups. As in the other tissues, inflammation was evaluated according to the presence of white cells. SHROB rats also showed greater lobular inflammation than Wistar.

Bottom Line: The quantitative data were expressed by mean ± SD and were compared among groups and treatments using ANOVA with post-hoc tests for parametric data and the U-Mann-Whitney for non-parametric data.Qualitative data were expressed in frequencies, and compared with contingency tables using χ² statistics.In both strains EPA:DHA 1:1 treatment improved inflammation related parameters in liver and kidney.

View Article: PubMed Central - HTML - PubMed

Affiliation: Unit of Pharmacology, Faculty of Medicine and Health Sciences, Rovira i Virgili University, Reus, Spain. nuria.taltavull@urv.cat.

ABSTRACT

Background: Marine polyunsaturated fatty acids, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) have been associated with improvement in the Metabolic Syndrome (MS). The aim of this study is to evaluate how three fish-oil diets with different eicosapentaenoic acid/docosahexaenoic acid ratios (EPA/DHA ratio) affect the histology of liver, kidney, adipose tissue and aorta in a preliminary morphological study. This work uses an animal model of metabolic syndrome in comparison with healthy animals in order to provide information about the best EPA:DHA ratio to prevent or to improve metabolic syndrome symptoms.

Methods: 35 Wistar rats, as a control, and 35 spontaneously hypertensive obese rats (SHROB) were fed for 13 weeks with 3 different supplementation of fish oil containing EPA and DHA ratios (1:1, 2:1 and 1:2, respectively). All samples were stained with haematoxylin/eosin stain, except aorta samples, which were stained also with Verhoeff and van Gieson's stain. A histological study was carried out to evaluate changes. These changes were statistically analyzed using SPSS IBM 19 software. The quantitative data were expressed by mean ± SD and were compared among groups and treatments using ANOVA with post-hoc tests for parametric data and the U-Mann-Whitney for non-parametric data. Qualitative data were expressed in frequencies, and compared with contingency tables using χ² statistics.

Results: EPA:DHA 1:1 treatment tended to improve the density and the wrinkling of elastic layers in SHROB rats. Only Wistar rats fed with EPA:DHA 1:1 treatment did not show mast cells in adipose tissue and has less kidney atrophy. In both strains EPA:DHA 1:1 treatment improved inflammation related parameters in liver and kidney.

Conclusions: EPA:DHA 1:1 treatment was the most beneficial treatment since improved many histological parameters in both groups of rats.

Show MeSH
Related in: MedlinePlus