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Toxicological Study of Ocimum sanctum Linn Leaves: Hematological, Biochemical, and Histopathological Studies.

Gautam MK, Goel RK - J Toxicol (2014)

Bottom Line: Subacute treatment with OSE did not show any change in body weight, food and water consumption, and hematological and biochemical profiles.In addition, no change was observed both in macroscopic and microscopic aspects of vital organs in rats.Our result showed that Ocimum sanctum extract could be safe for human use.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India.

ABSTRACT
The present study was aimed to study the acute and subacute toxicity studies with orally administered 50% ethanolic leaves extract of Ocimum sanctum Linn (OSE). In acute toxicity tests, four groups of mice (n = 6/group/sex) were orally treated with doses of 200, 600, and 2000 mg/kg, and general behavior, adverse effects, and mortality were recorded for up to 14 days. In subacute toxicity study, rats received OSE by gavage at the doses of 200, 400, and 800 mg/kg/day (n = 6/group/sex) for 28 days, and biochemical, hematological, and histopathological changes in tissues (liver, kidney, spleen, heart, and testis/ovary) were determined. OSE did not produce any hazardous symptoms or death and CNS and ANS toxicities in the acute toxicity test. Subacute treatment with OSE did not show any change in body weight, food and water consumption, and hematological and biochemical profiles. In addition, no change was observed both in macroscopic and microscopic aspects of vital organs in rats. Our result showed that Ocimum sanctum extract could be safe for human use.

No MeSH data available.


Related in: MedlinePlus

Histology of kidney (H&E, 100x) of control and OSE-treated animals. (a) Section of kidney from control animal showed normal size of glomeruli with normal tubules; ((b), (c), and (d)) kidney from OSE (200, 400, and 800 mg/kg)-treated animals exhibit normal size of glomeruli with normal tubules.
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fig2: Histology of kidney (H&E, 100x) of control and OSE-treated animals. (a) Section of kidney from control animal showed normal size of glomeruli with normal tubules; ((b), (c), and (d)) kidney from OSE (200, 400, and 800 mg/kg)-treated animals exhibit normal size of glomeruli with normal tubules.

Mentions: The organs like liver, kidney, heart, spleen, and testis or ovary isolated in various groups did not reveal any abnormalities in their gross examinations and difference in their mean weights both in treated and control groups (Table 4). The histological studies with liver, spleen, kidney, heart, and testis/ovary did not reveal any pathological changes after treatment even with higher dose of 800 mg dose of OSE when administered for 28 days (Figures 1, 2, 3, 4, 5, and 6).


Toxicological Study of Ocimum sanctum Linn Leaves: Hematological, Biochemical, and Histopathological Studies.

Gautam MK, Goel RK - J Toxicol (2014)

Histology of kidney (H&E, 100x) of control and OSE-treated animals. (a) Section of kidney from control animal showed normal size of glomeruli with normal tubules; ((b), (c), and (d)) kidney from OSE (200, 400, and 800 mg/kg)-treated animals exhibit normal size of glomeruli with normal tubules.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3927567&req=5

fig2: Histology of kidney (H&E, 100x) of control and OSE-treated animals. (a) Section of kidney from control animal showed normal size of glomeruli with normal tubules; ((b), (c), and (d)) kidney from OSE (200, 400, and 800 mg/kg)-treated animals exhibit normal size of glomeruli with normal tubules.
Mentions: The organs like liver, kidney, heart, spleen, and testis or ovary isolated in various groups did not reveal any abnormalities in their gross examinations and difference in their mean weights both in treated and control groups (Table 4). The histological studies with liver, spleen, kidney, heart, and testis/ovary did not reveal any pathological changes after treatment even with higher dose of 800 mg dose of OSE when administered for 28 days (Figures 1, 2, 3, 4, 5, and 6).

Bottom Line: Subacute treatment with OSE did not show any change in body weight, food and water consumption, and hematological and biochemical profiles.In addition, no change was observed both in macroscopic and microscopic aspects of vital organs in rats.Our result showed that Ocimum sanctum extract could be safe for human use.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India.

ABSTRACT
The present study was aimed to study the acute and subacute toxicity studies with orally administered 50% ethanolic leaves extract of Ocimum sanctum Linn (OSE). In acute toxicity tests, four groups of mice (n = 6/group/sex) were orally treated with doses of 200, 600, and 2000 mg/kg, and general behavior, adverse effects, and mortality were recorded for up to 14 days. In subacute toxicity study, rats received OSE by gavage at the doses of 200, 400, and 800 mg/kg/day (n = 6/group/sex) for 28 days, and biochemical, hematological, and histopathological changes in tissues (liver, kidney, spleen, heart, and testis/ovary) were determined. OSE did not produce any hazardous symptoms or death and CNS and ANS toxicities in the acute toxicity test. Subacute treatment with OSE did not show any change in body weight, food and water consumption, and hematological and biochemical profiles. In addition, no change was observed both in macroscopic and microscopic aspects of vital organs in rats. Our result showed that Ocimum sanctum extract could be safe for human use.

No MeSH data available.


Related in: MedlinePlus