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Electroanalysis of NADH Using Conducting and Redox Active Polymer/Carbon Nanotubes Modified Electrodes-A Review

View Article: PubMed Central - PubMed

ABSTRACT

Past few decades, conducting and redox active polymers play a critical role in the development of transducers for biosensing. It has been evidenced by increasing numerous reports on conducting and redox active polymers incorporated electrodes for assay of biomolcules. This review highlights the potential uses of electrogenerated polymer modified electrodes and polymer/carbon nanotubes composite modified electrodes for electroanalysis of reduced form of nicotinamide adenine dinuceltoide (NADH). In addition, carbon electrodes modified with organic and inorganic materials as modifier have been discussed in detail for the quantification of NADH based on mediator or mediator-less methods.

No MeSH data available.


Related in: MedlinePlus

Cyclic voltammograms of (a and b) poly(azure A) modified screen-printed carbon electrode in the absence (a) and presence (b and c) of 1mM NADH. Voltammogram (c) was obtained at a bare screen-printed carbon electrode. Scan rate: 5mVs-1. (Reproduced with permission from Gao et al., Talanta 2004, 62, 477–482)
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f2-sensors-08-00739: Cyclic voltammograms of (a and b) poly(azure A) modified screen-printed carbon electrode in the absence (a) and presence (b and c) of 1mM NADH. Voltammogram (c) was obtained at a bare screen-printed carbon electrode. Scan rate: 5mVs-1. (Reproduced with permission from Gao et al., Talanta 2004, 62, 477–482)

Mentions: The electrooxidation polymerization of phenothiazine derivatives, including azure A and toluidine blue O, has been studied at screen-printed carbon electrodes in neutral phosphate buffer. Both compounds yield strongly adsorbed electroactive polymer with reversible behavior and formal potential-closed to 0.04V at pH 6.9. Figure 2 shows typical voltammograms demonstrating the electrocatalytic activity of the poly(azure A) modified screen-printed carbon electrode for the oxidation of NADH. The reversible redox response of the poly(azure A) film can be observed over a wide range of potentials ca. -0.2 – 0.2V (versus Ag/AgCl) and its E0′ was located at -0.04V (versus Ag/AgCl) as shown in voltammogram (curve a). Voltammograms (curve b) observed in the presence of NADH showed a enhanced oxidation current and a large negative shift in the anodic peak potential of about 500mV, compared with that obtained at the bare screen-printed carbon electrode (curve c). These results demonstrated the electrocatalytic oxidation of NADH by the poly(azure A) film as described in equation 2. Further, the modified screen-printed carbon electrodes were found to be promising as an amperometric detector for the flow injection analysis (FIA) of NADH, typically with a dynamic range of 0.5–100μM [92].


Electroanalysis of NADH Using Conducting and Redox Active Polymer/Carbon Nanotubes Modified Electrodes-A Review
Cyclic voltammograms of (a and b) poly(azure A) modified screen-printed carbon electrode in the absence (a) and presence (b and c) of 1mM NADH. Voltammogram (c) was obtained at a bare screen-printed carbon electrode. Scan rate: 5mVs-1. (Reproduced with permission from Gao et al., Talanta 2004, 62, 477–482)
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3927499&req=5

f2-sensors-08-00739: Cyclic voltammograms of (a and b) poly(azure A) modified screen-printed carbon electrode in the absence (a) and presence (b and c) of 1mM NADH. Voltammogram (c) was obtained at a bare screen-printed carbon electrode. Scan rate: 5mVs-1. (Reproduced with permission from Gao et al., Talanta 2004, 62, 477–482)
Mentions: The electrooxidation polymerization of phenothiazine derivatives, including azure A and toluidine blue O, has been studied at screen-printed carbon electrodes in neutral phosphate buffer. Both compounds yield strongly adsorbed electroactive polymer with reversible behavior and formal potential-closed to 0.04V at pH 6.9. Figure 2 shows typical voltammograms demonstrating the electrocatalytic activity of the poly(azure A) modified screen-printed carbon electrode for the oxidation of NADH. The reversible redox response of the poly(azure A) film can be observed over a wide range of potentials ca. -0.2 – 0.2V (versus Ag/AgCl) and its E0′ was located at -0.04V (versus Ag/AgCl) as shown in voltammogram (curve a). Voltammograms (curve b) observed in the presence of NADH showed a enhanced oxidation current and a large negative shift in the anodic peak potential of about 500mV, compared with that obtained at the bare screen-printed carbon electrode (curve c). These results demonstrated the electrocatalytic oxidation of NADH by the poly(azure A) film as described in equation 2. Further, the modified screen-printed carbon electrodes were found to be promising as an amperometric detector for the flow injection analysis (FIA) of NADH, typically with a dynamic range of 0.5–100μM [92].

View Article: PubMed Central - PubMed

ABSTRACT

Past few decades, conducting and redox active polymers play a critical role in the development of transducers for biosensing. It has been evidenced by increasing numerous reports on conducting and redox active polymers incorporated electrodes for assay of biomolcules. This review highlights the potential uses of electrogenerated polymer modified electrodes and polymer/carbon nanotubes composite modified electrodes for electroanalysis of reduced form of nicotinamide adenine dinuceltoide (NADH). In addition, carbon electrodes modified with organic and inorganic materials as modifier have been discussed in detail for the quantification of NADH based on mediator or mediator-less methods.

No MeSH data available.


Related in: MedlinePlus