Limits...
Molecular identification of macroscopic and microscopic cysts of sarcocystis in sheep in north khorasan province, iran.

Bahari P, Salehi M, Seyedabadi M, Mohammadi A - Int J Mol Cell Med (2014)

Bottom Line: Genotypes were identified by BLAST search and homology analysis.Macrocysts were seen in two muscle tissues.Digestion method and PCR analysis revealed positive results in all samples taken from heart, liver, diaphragm, and muscle.

View Article: PubMed Central - PubMed

Affiliation: Vector-Borne Diseases Research Center, North Khorasan University of Medical & Laboratory of North Khorasan Veterinary Head office,Bojnurd, Iran.

ABSTRACT
Sarcocystis is an obligatory intracellular protozoan parasite which can infect humans and animals. Sheep are intermediate hosts of four Sarcocystis species: Sarcocystis tenella, Sarcocystis gigantea, Sarcocystis arieticanis, and Sarcocystis medusiformis The purpose of this study was to perform a molecular identification of the macroscopic and microscopic cysts of Sarcocystis in sheep. In this investigation, the macroscopic and microscopic cysts of Sarcocystis were assessed in slaughtered sheep. The digestion method was used for bradyzoites observation in heart, liver, diaphragm and muscle samples. PCR analysis was conducted on macroscopic and microscopic cysts and also all other samples. Sequencing was performed for ten PCR products. Genotypes were identified by BLAST search and homology analysis. Macrocysts were seen in two muscle tissues. Digestion method and PCR analysis revealed positive results in all samples taken from heart, liver, diaphragm, and muscle. Genotyping of ten tissue samples proved that the genotype of macroscopic belonged to Sarcocystis gigantea and microscopic cysts to Sarcocystis tenella. Microscopic cysts are more prevalent than macroscopic cysts and they can cause enormous economic losses.

No MeSH data available.


Related in: MedlinePlus

PCR-product of microscopic and macroscopic cyst.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3927390&req=5

Figure 4: PCR-product of microscopic and macroscopic cyst.

Mentions: In this study, two samples of muscle had macroscopic cysts (Figure1 and table1). In addition, these muscles were found to be infected with microscopic cysts by digestion method. The results of digestion method showed that all samples of heart, diaphragm, muscle and liver were infected with bradyzoites of Sarcocystis (Figure 2). PCR analysis of macrocysts and microcysts as well as all samples showed a specific 600 bp band on the agarose gel (Figures 3 and 4). The results obtained from sequencing of ten samples (2 hearts, 2 diaphragms, 4 muscles and 2 livers) showed that the genotype of macroscopic and microscopic cysts correspond to Sarcocystis gigantea and Sarcocystis tenella, respectively.


Molecular identification of macroscopic and microscopic cysts of sarcocystis in sheep in north khorasan province, iran.

Bahari P, Salehi M, Seyedabadi M, Mohammadi A - Int J Mol Cell Med (2014)

PCR-product of microscopic and macroscopic cyst.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3927390&req=5

Figure 4: PCR-product of microscopic and macroscopic cyst.
Mentions: In this study, two samples of muscle had macroscopic cysts (Figure1 and table1). In addition, these muscles were found to be infected with microscopic cysts by digestion method. The results of digestion method showed that all samples of heart, diaphragm, muscle and liver were infected with bradyzoites of Sarcocystis (Figure 2). PCR analysis of macrocysts and microcysts as well as all samples showed a specific 600 bp band on the agarose gel (Figures 3 and 4). The results obtained from sequencing of ten samples (2 hearts, 2 diaphragms, 4 muscles and 2 livers) showed that the genotype of macroscopic and microscopic cysts correspond to Sarcocystis gigantea and Sarcocystis tenella, respectively.

Bottom Line: Genotypes were identified by BLAST search and homology analysis.Macrocysts were seen in two muscle tissues.Digestion method and PCR analysis revealed positive results in all samples taken from heart, liver, diaphragm, and muscle.

View Article: PubMed Central - PubMed

Affiliation: Vector-Borne Diseases Research Center, North Khorasan University of Medical & Laboratory of North Khorasan Veterinary Head office,Bojnurd, Iran.

ABSTRACT
Sarcocystis is an obligatory intracellular protozoan parasite which can infect humans and animals. Sheep are intermediate hosts of four Sarcocystis species: Sarcocystis tenella, Sarcocystis gigantea, Sarcocystis arieticanis, and Sarcocystis medusiformis The purpose of this study was to perform a molecular identification of the macroscopic and microscopic cysts of Sarcocystis in sheep. In this investigation, the macroscopic and microscopic cysts of Sarcocystis were assessed in slaughtered sheep. The digestion method was used for bradyzoites observation in heart, liver, diaphragm and muscle samples. PCR analysis was conducted on macroscopic and microscopic cysts and also all other samples. Sequencing was performed for ten PCR products. Genotypes were identified by BLAST search and homology analysis. Macrocysts were seen in two muscle tissues. Digestion method and PCR analysis revealed positive results in all samples taken from heart, liver, diaphragm, and muscle. Genotyping of ten tissue samples proved that the genotype of macroscopic belonged to Sarcocystis gigantea and microscopic cysts to Sarcocystis tenella. Microscopic cysts are more prevalent than macroscopic cysts and they can cause enormous economic losses.

No MeSH data available.


Related in: MedlinePlus