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Sulfated polysaccharides purified from two species of padina improve collagen and epidermis formation in the rat.

Kordjazi M, Shabanpour B, Zabihi E, Faramarzi MA, Feizi F, Ahmadi Gavlighi H, Feghhi MA, Hosseini SA - Int J Mol Cell Med (2013)

Bottom Line: The sulfated polysaccharides extracted from two algae species habitats in Persian Gulf were studied in vivo for their effects on collagen formation and epidermal regeneration.The polysaccharides purified from P. boergesenii had higher sulfate content (32.6±1%) compared to P. tetrastromatica (19±1%).The superior healing properties of P. boergesenii polysaccharides might be correlated to its higher sulfate content.

View Article: PubMed Central - PubMed

Affiliation: Department of Fisheries, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.

ABSTRACT
Sulfated polysaccharides have shown promising effects on wound healing processes along with many other biological activities. The sulfated polysaccharides extracted from two algae species habitats in Persian Gulf were studied in vivo for their effects on collagen formation and epidermal regeneration. The polysaccharides were purified from aqueous extracts of P. tetrastromatica and P. boergesenii using CaCl2 and ethanol precipitation. The sulfate content of each polysaccharide was determined. Two identical wounds (either burn or excision) were made on the back of 4 groups of male Wistar rats (10 rats per group) under anesthesia. The algal polysaccharide ointments (2%) were applied twice daily on one side and the other wound was treated with Eucerin (as control). The rats were sacrificed on day 7 or 14, and then the wound samples were examined for epidermal thickness by light microscope. Furthermore, hydroxyproline content (as a marker of collagen formation) was spectro-photometrically measured. The polysaccharides purified from P. boergesenii had higher sulfate content (32.6±1%) compared to P. tetrastromatica (19±1%). Both algal polysaccharides showed some improvements in collagen formation (hydroxyproline content) and epidermal thickness in both wound models compared to the vehicle. The sulfated polysaccharides purified from P. tetrastromatica and P. boergesenii seaweeds are able to induce collagen formation and epidermal regeneration in the two wound models. The superior healing properties of P. boergesenii polysaccharides might be correlated to its higher sulfate content. Both algal polysaccharides are good candidates for wound healing clinical trials.

No MeSH data available.


Related in: MedlinePlus

The photographs of excisional wounds treated by two different algal extract ointments on different days after treat-ment. A: Control wound corresponding to P. tetrastromatica; B: Wound treated by P. tetrastromatica; C: Control wound corresponding to P. boergesenii; D: Wound treated by P. boergesenii. Both algal extract ointments (B, D) have signifi-cant effects on wound healing compared to the control (A, C). The healing effect is obvious from day 7th after excision
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Figure 4: The photographs of excisional wounds treated by two different algal extract ointments on different days after treat-ment. A: Control wound corresponding to P. tetrastromatica; B: Wound treated by P. tetrastromatica; C: Control wound corresponding to P. boergesenii; D: Wound treated by P. boergesenii. Both algal extract ointments (B, D) have signifi-cant effects on wound healing compared to the control (A, C). The healing effect is obvious from day 7th after excision

Mentions: Since the polysaccharides method of extraction/purification was specific to fucoidans, it could be assumed that the sulfated polysaccharides purified by this procedure mostly belong to fucoidans. The sulfate content of P. boergesenii extracted polysaccharides (32.6±1%) was quite higher than P. tetrastromatica (19±1%). The higher sulfate content might be attributed to the better wound healing properties (13, 16). Eventually the better healing effects of P. boergesenii on cellular and molecular changes in burn or excision wounds could be observed even by gross daily examination of the wounds (Fig. 3, 4).


Sulfated polysaccharides purified from two species of padina improve collagen and epidermis formation in the rat.

Kordjazi M, Shabanpour B, Zabihi E, Faramarzi MA, Feizi F, Ahmadi Gavlighi H, Feghhi MA, Hosseini SA - Int J Mol Cell Med (2013)

The photographs of excisional wounds treated by two different algal extract ointments on different days after treat-ment. A: Control wound corresponding to P. tetrastromatica; B: Wound treated by P. tetrastromatica; C: Control wound corresponding to P. boergesenii; D: Wound treated by P. boergesenii. Both algal extract ointments (B, D) have signifi-cant effects on wound healing compared to the control (A, C). The healing effect is obvious from day 7th after excision
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3927382&req=5

Figure 4: The photographs of excisional wounds treated by two different algal extract ointments on different days after treat-ment. A: Control wound corresponding to P. tetrastromatica; B: Wound treated by P. tetrastromatica; C: Control wound corresponding to P. boergesenii; D: Wound treated by P. boergesenii. Both algal extract ointments (B, D) have signifi-cant effects on wound healing compared to the control (A, C). The healing effect is obvious from day 7th after excision
Mentions: Since the polysaccharides method of extraction/purification was specific to fucoidans, it could be assumed that the sulfated polysaccharides purified by this procedure mostly belong to fucoidans. The sulfate content of P. boergesenii extracted polysaccharides (32.6±1%) was quite higher than P. tetrastromatica (19±1%). The higher sulfate content might be attributed to the better wound healing properties (13, 16). Eventually the better healing effects of P. boergesenii on cellular and molecular changes in burn or excision wounds could be observed even by gross daily examination of the wounds (Fig. 3, 4).

Bottom Line: The sulfated polysaccharides extracted from two algae species habitats in Persian Gulf were studied in vivo for their effects on collagen formation and epidermal regeneration.The polysaccharides purified from P. boergesenii had higher sulfate content (32.6±1%) compared to P. tetrastromatica (19±1%).The superior healing properties of P. boergesenii polysaccharides might be correlated to its higher sulfate content.

View Article: PubMed Central - PubMed

Affiliation: Department of Fisheries, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.

ABSTRACT
Sulfated polysaccharides have shown promising effects on wound healing processes along with many other biological activities. The sulfated polysaccharides extracted from two algae species habitats in Persian Gulf were studied in vivo for their effects on collagen formation and epidermal regeneration. The polysaccharides were purified from aqueous extracts of P. tetrastromatica and P. boergesenii using CaCl2 and ethanol precipitation. The sulfate content of each polysaccharide was determined. Two identical wounds (either burn or excision) were made on the back of 4 groups of male Wistar rats (10 rats per group) under anesthesia. The algal polysaccharide ointments (2%) were applied twice daily on one side and the other wound was treated with Eucerin (as control). The rats were sacrificed on day 7 or 14, and then the wound samples were examined for epidermal thickness by light microscope. Furthermore, hydroxyproline content (as a marker of collagen formation) was spectro-photometrically measured. The polysaccharides purified from P. boergesenii had higher sulfate content (32.6±1%) compared to P. tetrastromatica (19±1%). Both algal polysaccharides showed some improvements in collagen formation (hydroxyproline content) and epidermal thickness in both wound models compared to the vehicle. The sulfated polysaccharides purified from P. tetrastromatica and P. boergesenii seaweeds are able to induce collagen formation and epidermal regeneration in the two wound models. The superior healing properties of P. boergesenii polysaccharides might be correlated to its higher sulfate content. Both algal polysaccharides are good candidates for wound healing clinical trials.

No MeSH data available.


Related in: MedlinePlus