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Facts in artifacts.

Bindhu P, Krishnapillai R, Thomas P, Jayanthi P - J Oral Maxillofac Pathol (2013)

Bottom Line: Defects are common in tissue sections as a result of faulty procedures.They lead to misinterpretation of histopathological diagnosis but at times they throw limelight into diagnosis.This paper attempts to put together all the facts regarding the various artifacts that are encountered in histopathology.

View Article: PubMed Central - PubMed

Affiliation: Departments of Oral Pathology and Microbiology, Annoor Dental College, Muvattupuzha, Ernakulam, Kerala, India.

ABSTRACT
Examination of microscopic sections of animal tissues reveals facts which are not always related to its normal histology or pathology. Processing of tissue specimens consists of lengthy procedures from the stage of surgical removal to the stained and mounted microscopic sections. Defects are common in tissue sections as a result of faulty procedures. These defects are referred to as artifacts. They lead to misinterpretation of histopathological diagnosis but at times they throw limelight into diagnosis. This paper attempts to put together all the facts regarding the various artifacts that are encountered in histopathology.

No MeSH data available.


Related in: MedlinePlus

(a) Clefts formed as a result of dissolution of cholesterol crystals. (b) Max Joseph space formed as a result of basilar degeneration (H&E stain, ×100)
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Figure 5: (a) Clefts formed as a result of dissolution of cholesterol crystals. (b) Max Joseph space formed as a result of basilar degeneration (H&E stain, ×100)

Mentions: Cholesterol clefts in radicular cysts or periapical granuloma are produced as a result of dissolution of lipids during processing that leave behind needle like spaces [Figure 5a]. Lacunar cells, the diagnostic clue to nodular sclerosis, a variant of Hodgkin's lymphoma is an artifact induced by formalin fixation and absent with other fixatives. These cells are formed by retraction of cytoplasm towards the nuclear membrane thus giving the appearance of cells enclosed within lacunae.[17] Max Joseph Space (Caspary Joseph Space) associated with lichen planus is an artifactual space in the subepithelial region caused during processing and is attributed to basal cell degeneration[18] [Figure 5b]. Formalin induced fluorescence can detect melanin pigment in amelanotic melanoma where melanin is not demonstrable in routine hematoxylin and eosin (H and E) section.[19]


Facts in artifacts.

Bindhu P, Krishnapillai R, Thomas P, Jayanthi P - J Oral Maxillofac Pathol (2013)

(a) Clefts formed as a result of dissolution of cholesterol crystals. (b) Max Joseph space formed as a result of basilar degeneration (H&E stain, ×100)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3927342&req=5

Figure 5: (a) Clefts formed as a result of dissolution of cholesterol crystals. (b) Max Joseph space formed as a result of basilar degeneration (H&E stain, ×100)
Mentions: Cholesterol clefts in radicular cysts or periapical granuloma are produced as a result of dissolution of lipids during processing that leave behind needle like spaces [Figure 5a]. Lacunar cells, the diagnostic clue to nodular sclerosis, a variant of Hodgkin's lymphoma is an artifact induced by formalin fixation and absent with other fixatives. These cells are formed by retraction of cytoplasm towards the nuclear membrane thus giving the appearance of cells enclosed within lacunae.[17] Max Joseph Space (Caspary Joseph Space) associated with lichen planus is an artifactual space in the subepithelial region caused during processing and is attributed to basal cell degeneration[18] [Figure 5b]. Formalin induced fluorescence can detect melanin pigment in amelanotic melanoma where melanin is not demonstrable in routine hematoxylin and eosin (H and E) section.[19]

Bottom Line: Defects are common in tissue sections as a result of faulty procedures.They lead to misinterpretation of histopathological diagnosis but at times they throw limelight into diagnosis.This paper attempts to put together all the facts regarding the various artifacts that are encountered in histopathology.

View Article: PubMed Central - PubMed

Affiliation: Departments of Oral Pathology and Microbiology, Annoor Dental College, Muvattupuzha, Ernakulam, Kerala, India.

ABSTRACT
Examination of microscopic sections of animal tissues reveals facts which are not always related to its normal histology or pathology. Processing of tissue specimens consists of lengthy procedures from the stage of surgical removal to the stained and mounted microscopic sections. Defects are common in tissue sections as a result of faulty procedures. These defects are referred to as artifacts. They lead to misinterpretation of histopathological diagnosis but at times they throw limelight into diagnosis. This paper attempts to put together all the facts regarding the various artifacts that are encountered in histopathology.

No MeSH data available.


Related in: MedlinePlus