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Facts in artifacts.

Bindhu P, Krishnapillai R, Thomas P, Jayanthi P - J Oral Maxillofac Pathol (2013)

Bottom Line: Defects are common in tissue sections as a result of faulty procedures.They lead to misinterpretation of histopathological diagnosis but at times they throw limelight into diagnosis.This paper attempts to put together all the facts regarding the various artifacts that are encountered in histopathology.

View Article: PubMed Central - PubMed

Affiliation: Departments of Oral Pathology and Microbiology, Annoor Dental College, Muvattupuzha, Ernakulam, Kerala, India.

ABSTRACT
Examination of microscopic sections of animal tissues reveals facts which are not always related to its normal histology or pathology. Processing of tissue specimens consists of lengthy procedures from the stage of surgical removal to the stained and mounted microscopic sections. Defects are common in tissue sections as a result of faulty procedures. These defects are referred to as artifacts. They lead to misinterpretation of histopathological diagnosis but at times they throw limelight into diagnosis. This paper attempts to put together all the facts regarding the various artifacts that are encountered in histopathology.

No MeSH data available.


(a) Displacement of bone during microtomy in association with the use of dull knife. (b) Microscopic section showing folding. (c) Residual wax within the stained section. (d) Stain deposits within salivary gland tissue (H&E stain, ×100)
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Figure 3: (a) Displacement of bone during microtomy in association with the use of dull knife. (b) Microscopic section showing folding. (c) Residual wax within the stained section. (d) Stain deposits within salivary gland tissue (H&E stain, ×100)

Mentions: Thick and thin sections and chatter/venetian blind artifact are formed as a result of loosely attached microtome knife or tissue block, steep angle of the cutting knife, hard tissue or wax, and presence of calcification in tissues[15] [Figure 2b]. Scratch lines appear in the sections due to small nicks in the knife edge, large knife clearance angle, hard material embedded in the wax or hard material in the tissue[15] [Figure 2c]. Crumbling of sections occur on cutting if the knife is blunt or the wax is too soft or due to contamination of wax with clearing agent or water or due to slow cooling of wax[15] [Figure 2d]. Loss of bevel on the knife edge produces compression of the block which in turn leads to formation of creases in the cut sections.[15] Displacement of tissue components especially bone during microtomy is a common finding in association with the use of dull knife, soft embedding medium, rough sectioning, incorrect blotting and poor adhesion of sections to the glass slide [Figure 3a].


Facts in artifacts.

Bindhu P, Krishnapillai R, Thomas P, Jayanthi P - J Oral Maxillofac Pathol (2013)

(a) Displacement of bone during microtomy in association with the use of dull knife. (b) Microscopic section showing folding. (c) Residual wax within the stained section. (d) Stain deposits within salivary gland tissue (H&E stain, ×100)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3927342&req=5

Figure 3: (a) Displacement of bone during microtomy in association with the use of dull knife. (b) Microscopic section showing folding. (c) Residual wax within the stained section. (d) Stain deposits within salivary gland tissue (H&E stain, ×100)
Mentions: Thick and thin sections and chatter/venetian blind artifact are formed as a result of loosely attached microtome knife or tissue block, steep angle of the cutting knife, hard tissue or wax, and presence of calcification in tissues[15] [Figure 2b]. Scratch lines appear in the sections due to small nicks in the knife edge, large knife clearance angle, hard material embedded in the wax or hard material in the tissue[15] [Figure 2c]. Crumbling of sections occur on cutting if the knife is blunt or the wax is too soft or due to contamination of wax with clearing agent or water or due to slow cooling of wax[15] [Figure 2d]. Loss of bevel on the knife edge produces compression of the block which in turn leads to formation of creases in the cut sections.[15] Displacement of tissue components especially bone during microtomy is a common finding in association with the use of dull knife, soft embedding medium, rough sectioning, incorrect blotting and poor adhesion of sections to the glass slide [Figure 3a].

Bottom Line: Defects are common in tissue sections as a result of faulty procedures.They lead to misinterpretation of histopathological diagnosis but at times they throw limelight into diagnosis.This paper attempts to put together all the facts regarding the various artifacts that are encountered in histopathology.

View Article: PubMed Central - PubMed

Affiliation: Departments of Oral Pathology and Microbiology, Annoor Dental College, Muvattupuzha, Ernakulam, Kerala, India.

ABSTRACT
Examination of microscopic sections of animal tissues reveals facts which are not always related to its normal histology or pathology. Processing of tissue specimens consists of lengthy procedures from the stage of surgical removal to the stained and mounted microscopic sections. Defects are common in tissue sections as a result of faulty procedures. These defects are referred to as artifacts. They lead to misinterpretation of histopathological diagnosis but at times they throw limelight into diagnosis. This paper attempts to put together all the facts regarding the various artifacts that are encountered in histopathology.

No MeSH data available.