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Facts in artifacts.

Bindhu P, Krishnapillai R, Thomas P, Jayanthi P - J Oral Maxillofac Pathol (2013)

Bottom Line: Defects are common in tissue sections as a result of faulty procedures.They lead to misinterpretation of histopathological diagnosis but at times they throw limelight into diagnosis.This paper attempts to put together all the facts regarding the various artifacts that are encountered in histopathology.

View Article: PubMed Central - PubMed

Affiliation: Departments of Oral Pathology and Microbiology, Annoor Dental College, Muvattupuzha, Ernakulam, Kerala, India.

ABSTRACT
Examination of microscopic sections of animal tissues reveals facts which are not always related to its normal histology or pathology. Processing of tissue specimens consists of lengthy procedures from the stage of surgical removal to the stained and mounted microscopic sections. Defects are common in tissue sections as a result of faulty procedures. These defects are referred to as artifacts. They lead to misinterpretation of histopathological diagnosis but at times they throw limelight into diagnosis. This paper attempts to put together all the facts regarding the various artifacts that are encountered in histopathology.

No MeSH data available.


Related in: MedlinePlus

(a) Tangential section of epithelium caused by improper orientation. (b) Thick and thin section formed due to loosely attached microtome knife. (c) Knife scoring appeared in the section due to a small nick in the knife edge. (d) Folds and wrinkles within the histological section produced by a blunt microtome knife (H&E stain, ×100)
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Figure 2: (a) Tangential section of epithelium caused by improper orientation. (b) Thick and thin section formed due to loosely attached microtome knife. (c) Knife scoring appeared in the section due to a small nick in the knife edge. (d) Folds and wrinkles within the histological section produced by a blunt microtome knife (H&E stain, ×100)

Mentions: Floaters or cross-contamination artifacts are small pieces of tissue that appear on a slide which do not belong to that particular area and have floated in during grossing, processing or floatation of cut-sections. They may also arise from sloppy procedures on the cutting bench such as dirty towels, instruments or gloves that have remnants of tissue that is carried over to the next case. Therefore, it is essential to do only one specimen at a time and clean thoroughly before opening the container of the next case. Thin and narrow tissue specimens tend to curl during processing. This will cause difficulty in orienting the tissue while embedding leading to formation of tangential sections [Figure 2a].


Facts in artifacts.

Bindhu P, Krishnapillai R, Thomas P, Jayanthi P - J Oral Maxillofac Pathol (2013)

(a) Tangential section of epithelium caused by improper orientation. (b) Thick and thin section formed due to loosely attached microtome knife. (c) Knife scoring appeared in the section due to a small nick in the knife edge. (d) Folds and wrinkles within the histological section produced by a blunt microtome knife (H&E stain, ×100)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3927342&req=5

Figure 2: (a) Tangential section of epithelium caused by improper orientation. (b) Thick and thin section formed due to loosely attached microtome knife. (c) Knife scoring appeared in the section due to a small nick in the knife edge. (d) Folds and wrinkles within the histological section produced by a blunt microtome knife (H&E stain, ×100)
Mentions: Floaters or cross-contamination artifacts are small pieces of tissue that appear on a slide which do not belong to that particular area and have floated in during grossing, processing or floatation of cut-sections. They may also arise from sloppy procedures on the cutting bench such as dirty towels, instruments or gloves that have remnants of tissue that is carried over to the next case. Therefore, it is essential to do only one specimen at a time and clean thoroughly before opening the container of the next case. Thin and narrow tissue specimens tend to curl during processing. This will cause difficulty in orienting the tissue while embedding leading to formation of tangential sections [Figure 2a].

Bottom Line: Defects are common in tissue sections as a result of faulty procedures.They lead to misinterpretation of histopathological diagnosis but at times they throw limelight into diagnosis.This paper attempts to put together all the facts regarding the various artifacts that are encountered in histopathology.

View Article: PubMed Central - PubMed

Affiliation: Departments of Oral Pathology and Microbiology, Annoor Dental College, Muvattupuzha, Ernakulam, Kerala, India.

ABSTRACT
Examination of microscopic sections of animal tissues reveals facts which are not always related to its normal histology or pathology. Processing of tissue specimens consists of lengthy procedures from the stage of surgical removal to the stained and mounted microscopic sections. Defects are common in tissue sections as a result of faulty procedures. These defects are referred to as artifacts. They lead to misinterpretation of histopathological diagnosis but at times they throw limelight into diagnosis. This paper attempts to put together all the facts regarding the various artifacts that are encountered in histopathology.

No MeSH data available.


Related in: MedlinePlus