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Estimation and correlative study of salivary nitrate and nitrite in tobacco related oral squamous carcinoma and submucous fibrosis.

Shende V, Biviji A, Akarte N - J Oral Maxillofac Pathol (2013)

Bottom Line: Betel nut chewing plays significant role in etiology of OSMF.The nut alkaloids have been shown experimentally to result in stimulation of collagen synthesis by fibroblasts in vitro, which can induce precancerous conditions.We conclude that the major inducer of oral squamous cell carcinoma (OSCC) is exposure to tobacco.

View Article: PubMed Central - PubMed

Affiliation: Department Oral Pathology and Microbiology, KMCT Dental College, Nagpur, India.

ABSTRACT

Unlabelled: Oral cancer is one of the ten leading cancers of the world. In India, it is one of the common cancers and is an important public health problem. Tobacco plays significant role in etiology of oral squamous carcinoma. Tobacco which is chewed or smoked contains many alkaloids which are known carcinogens. Oral submucous fibrosis (OSMF) is a disease of the Indian subcontinent, which through immigration has a worldwide distribution. Betel nut chewing plays significant role in etiology of OSMF. The nut alkaloids have been shown experimentally to result in stimulation of collagen synthesis by fibroblasts in vitro, which can induce precancerous conditions.

Materials and methods: The present study was undertaken to detect nitrate and nitrite factor in saliva of cases with oral carcinoma, OSMF and normal individuals without any habits and to determine whether increased salivary nitrate and nitrite level is significant in oral carcinoma and submucous fibrosis using biochemical parameters.

Conclusion: We conclude that the major inducer of oral squamous cell carcinoma (OSCC) is exposure to tobacco. Recent studies have demonstrated that oxidative and nitrosative stress contributes to the development of oral carcinogenesis through deoxyribonucleic acid (DNA) damage. Salivary composition of OSCC patients is substantially altered with respect to free radical-involved mechanisms.

No MeSH data available.


Related in: MedlinePlus

Salivary nitrate level
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Figure 1: Salivary nitrate level

Mentions: Aliquots of standard nitrate solution in the range of 0.1-10 mg NO3-N/L were taken using 50 ml as a final volume. The solution was evaporated to dryness on water bath. The residue was dissolved using glass rod with 2 ml phenol disulfonic acid reagent. It was diluted with distilled water and transferred to Nessler tube. Then 8 ml of 12N KOH was added and made to 100 ml. The color developed was measured at 410 nm with a light path of 10 m. Calibration curve was drawn on cm-cm graph paper using optical density against concentration of nitrate. This graph was used for calculating nitrate in the samples [Figure 1].


Estimation and correlative study of salivary nitrate and nitrite in tobacco related oral squamous carcinoma and submucous fibrosis.

Shende V, Biviji A, Akarte N - J Oral Maxillofac Pathol (2013)

Salivary nitrate level
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3927339&req=5

Figure 1: Salivary nitrate level
Mentions: Aliquots of standard nitrate solution in the range of 0.1-10 mg NO3-N/L were taken using 50 ml as a final volume. The solution was evaporated to dryness on water bath. The residue was dissolved using glass rod with 2 ml phenol disulfonic acid reagent. It was diluted with distilled water and transferred to Nessler tube. Then 8 ml of 12N KOH was added and made to 100 ml. The color developed was measured at 410 nm with a light path of 10 m. Calibration curve was drawn on cm-cm graph paper using optical density against concentration of nitrate. This graph was used for calculating nitrate in the samples [Figure 1].

Bottom Line: Betel nut chewing plays significant role in etiology of OSMF.The nut alkaloids have been shown experimentally to result in stimulation of collagen synthesis by fibroblasts in vitro, which can induce precancerous conditions.We conclude that the major inducer of oral squamous cell carcinoma (OSCC) is exposure to tobacco.

View Article: PubMed Central - PubMed

Affiliation: Department Oral Pathology and Microbiology, KMCT Dental College, Nagpur, India.

ABSTRACT

Unlabelled: Oral cancer is one of the ten leading cancers of the world. In India, it is one of the common cancers and is an important public health problem. Tobacco plays significant role in etiology of oral squamous carcinoma. Tobacco which is chewed or smoked contains many alkaloids which are known carcinogens. Oral submucous fibrosis (OSMF) is a disease of the Indian subcontinent, which through immigration has a worldwide distribution. Betel nut chewing plays significant role in etiology of OSMF. The nut alkaloids have been shown experimentally to result in stimulation of collagen synthesis by fibroblasts in vitro, which can induce precancerous conditions.

Materials and methods: The present study was undertaken to detect nitrate and nitrite factor in saliva of cases with oral carcinoma, OSMF and normal individuals without any habits and to determine whether increased salivary nitrate and nitrite level is significant in oral carcinoma and submucous fibrosis using biochemical parameters.

Conclusion: We conclude that the major inducer of oral squamous cell carcinoma (OSCC) is exposure to tobacco. Recent studies have demonstrated that oxidative and nitrosative stress contributes to the development of oral carcinogenesis through deoxyribonucleic acid (DNA) damage. Salivary composition of OSCC patients is substantially altered with respect to free radical-involved mechanisms.

No MeSH data available.


Related in: MedlinePlus