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VEGF and CD 34: A correlation between tumor angiogenesis and microvessel density-an immunohistochemical study.

Kukreja I, Kapoor P, Deshmukh R, Kulkarni V - J Oral Maxillofac Pathol (2013)

Bottom Line: Thirty-three cases of OSCC were stained with hematoxylin and eosin, (H and E) to confirm the diagnosis and immunohistochemically using VEGF and CD 34 antibody.The result was subjected to statistical analysis using analysis of variance (ANOVA) test and Fisher's exact test.Tumor MVD was found to be independent of expression of VEGF.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral and Maxillofacial Pathology, Bharati Vidyapeeth Dental college and Hospital, Pune, Maharashtra, India.

ABSTRACT

Background: Angiogenesis is the formation of new vessels from preexisting ones which takes place by capillary sprouting. It is seen in healing, at sites of injury and collateral circulation in ischemia. It is also seen in tumors, as once the original blood supply of a tumor is exhausted it cannot grow without further blood supply. Also as the angiogenic capacity of a tumor increases, its microvasculature, that is, microvessel density (MVD) also increases. Based on this literary evidence we carried out an immunohistochemical (IHC) study to observe the relationship between the expression of vascular endothelial growth factor (VEGF) [angiogenesis] and CD 34 [MVD] in oral squamous cell carcinoma (OSCC).

Aim: To evaluate the correlation between expression of VEGF and CD 34, the role of MVD in progression of OSCC and to compare the degree of angiogenesis in different grades of OSCC.

Settings and design: In this study we observed the relation between angiogenesis and MVD and the overall effect of this on oral cancer.

Materials and methods: Thirty-three cases of OSCC were stained with hematoxylin and eosin, (H and E) to confirm the diagnosis and immunohistochemically using VEGF and CD 34 antibody. The slides were evaluated for positivity and intensity of staining.

Statistical analysis: The result was subjected to statistical analysis using analysis of variance (ANOVA) test and Fisher's exact test.

Results: VEGF positivity as well as MVD was found to be independent of the grade of the tumor. Tumor MVD was found to be independent of expression of VEGF.

No MeSH data available.


Related in: MedlinePlus

Comparison of MVD count with respect to VEGF intensity staining
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Figure 10: Comparison of MVD count with respect to VEGF intensity staining

Mentions: Table 3 and Figure 10 show the comparison of MVD count with respect to VEGF intensity staining. Out of a total of 33 cases, four cases show negative staining with VEGF and a mean MVD count of 11.42 ± 2.50 SD, twenty cases show a staining intensity of 1+ with VEGF and a mean MVD count of 9.35 ± 5.43 SD, eight cases show a staining intensity of 2+ and a mean MVD count of 10.17 ± 5.20 and only one case shows a staining intensity of 3+ with VEGF and a mean MVD count of 9.33 ± 0.00 SD. By using ANOVA test P value is 0.897, that is, >0.05; therefore, there is no significant correlation between mean CD 34 counts with respect to VEGF intensity staining.


VEGF and CD 34: A correlation between tumor angiogenesis and microvessel density-an immunohistochemical study.

Kukreja I, Kapoor P, Deshmukh R, Kulkarni V - J Oral Maxillofac Pathol (2013)

Comparison of MVD count with respect to VEGF intensity staining
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3927337&req=5

Figure 10: Comparison of MVD count with respect to VEGF intensity staining
Mentions: Table 3 and Figure 10 show the comparison of MVD count with respect to VEGF intensity staining. Out of a total of 33 cases, four cases show negative staining with VEGF and a mean MVD count of 11.42 ± 2.50 SD, twenty cases show a staining intensity of 1+ with VEGF and a mean MVD count of 9.35 ± 5.43 SD, eight cases show a staining intensity of 2+ and a mean MVD count of 10.17 ± 5.20 and only one case shows a staining intensity of 3+ with VEGF and a mean MVD count of 9.33 ± 0.00 SD. By using ANOVA test P value is 0.897, that is, >0.05; therefore, there is no significant correlation between mean CD 34 counts with respect to VEGF intensity staining.

Bottom Line: Thirty-three cases of OSCC were stained with hematoxylin and eosin, (H and E) to confirm the diagnosis and immunohistochemically using VEGF and CD 34 antibody.The result was subjected to statistical analysis using analysis of variance (ANOVA) test and Fisher's exact test.Tumor MVD was found to be independent of expression of VEGF.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral and Maxillofacial Pathology, Bharati Vidyapeeth Dental college and Hospital, Pune, Maharashtra, India.

ABSTRACT

Background: Angiogenesis is the formation of new vessels from preexisting ones which takes place by capillary sprouting. It is seen in healing, at sites of injury and collateral circulation in ischemia. It is also seen in tumors, as once the original blood supply of a tumor is exhausted it cannot grow without further blood supply. Also as the angiogenic capacity of a tumor increases, its microvasculature, that is, microvessel density (MVD) also increases. Based on this literary evidence we carried out an immunohistochemical (IHC) study to observe the relationship between the expression of vascular endothelial growth factor (VEGF) [angiogenesis] and CD 34 [MVD] in oral squamous cell carcinoma (OSCC).

Aim: To evaluate the correlation between expression of VEGF and CD 34, the role of MVD in progression of OSCC and to compare the degree of angiogenesis in different grades of OSCC.

Settings and design: In this study we observed the relation between angiogenesis and MVD and the overall effect of this on oral cancer.

Materials and methods: Thirty-three cases of OSCC were stained with hematoxylin and eosin, (H and E) to confirm the diagnosis and immunohistochemically using VEGF and CD 34 antibody. The slides were evaluated for positivity and intensity of staining.

Statistical analysis: The result was subjected to statistical analysis using analysis of variance (ANOVA) test and Fisher's exact test.

Results: VEGF positivity as well as MVD was found to be independent of the grade of the tumor. Tumor MVD was found to be independent of expression of VEGF.

No MeSH data available.


Related in: MedlinePlus