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VEGF and CD 34: A correlation between tumor angiogenesis and microvessel density-an immunohistochemical study.

Kukreja I, Kapoor P, Deshmukh R, Kulkarni V - J Oral Maxillofac Pathol (2013)

Bottom Line: Thirty-three cases of OSCC were stained with hematoxylin and eosin, (H and E) to confirm the diagnosis and immunohistochemically using VEGF and CD 34 antibody.The result was subjected to statistical analysis using analysis of variance (ANOVA) test and Fisher's exact test.Tumor MVD was found to be independent of expression of VEGF.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral and Maxillofacial Pathology, Bharati Vidyapeeth Dental college and Hospital, Pune, Maharashtra, India.

ABSTRACT

Background: Angiogenesis is the formation of new vessels from preexisting ones which takes place by capillary sprouting. It is seen in healing, at sites of injury and collateral circulation in ischemia. It is also seen in tumors, as once the original blood supply of a tumor is exhausted it cannot grow without further blood supply. Also as the angiogenic capacity of a tumor increases, its microvasculature, that is, microvessel density (MVD) also increases. Based on this literary evidence we carried out an immunohistochemical (IHC) study to observe the relationship between the expression of vascular endothelial growth factor (VEGF) [angiogenesis] and CD 34 [MVD] in oral squamous cell carcinoma (OSCC).

Aim: To evaluate the correlation between expression of VEGF and CD 34, the role of MVD in progression of OSCC and to compare the degree of angiogenesis in different grades of OSCC.

Settings and design: In this study we observed the relation between angiogenesis and MVD and the overall effect of this on oral cancer.

Materials and methods: Thirty-three cases of OSCC were stained with hematoxylin and eosin, (H and E) to confirm the diagnosis and immunohistochemically using VEGF and CD 34 antibody. The slides were evaluated for positivity and intensity of staining.

Statistical analysis: The result was subjected to statistical analysis using analysis of variance (ANOVA) test and Fisher's exact test.

Results: VEGF positivity as well as MVD was found to be independent of the grade of the tumor. Tumor MVD was found to be independent of expression of VEGF.

No MeSH data available.


Related in: MedlinePlus

Histopathological image shows CD 34 staining for moderately differentiated squamous cell carcinoma (IHC stain, ×400)
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Figure 8: Histopathological image shows CD 34 staining for moderately differentiated squamous cell carcinoma (IHC stain, ×400)

Mentions: Table 2 and Figure 6 show the comparison of MVD count with respect to grade of the tumor. Poorly differentiated OSCC shows an average count of 9.64 with SD of ±4.22 [Figure 7]. Moderately differentiated OSCC shows an average of 5.63 with SD of ±3.04 [Figure 8]. Well differentiated OSCC shows an average count of 12.08 with SD of ±5.39 [Figure 9]. By using analysis of variance (ANOVA) test P value is 0.027, that is, <0.05. Therefore, the results are statistically significant. No correlation is seen between the MVD counts and the grade of the tumor. However, it is observed that well-differentiated OSCC showed the maximum MVD.


VEGF and CD 34: A correlation between tumor angiogenesis and microvessel density-an immunohistochemical study.

Kukreja I, Kapoor P, Deshmukh R, Kulkarni V - J Oral Maxillofac Pathol (2013)

Histopathological image shows CD 34 staining for moderately differentiated squamous cell carcinoma (IHC stain, ×400)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3927337&req=5

Figure 8: Histopathological image shows CD 34 staining for moderately differentiated squamous cell carcinoma (IHC stain, ×400)
Mentions: Table 2 and Figure 6 show the comparison of MVD count with respect to grade of the tumor. Poorly differentiated OSCC shows an average count of 9.64 with SD of ±4.22 [Figure 7]. Moderately differentiated OSCC shows an average of 5.63 with SD of ±3.04 [Figure 8]. Well differentiated OSCC shows an average count of 12.08 with SD of ±5.39 [Figure 9]. By using analysis of variance (ANOVA) test P value is 0.027, that is, <0.05. Therefore, the results are statistically significant. No correlation is seen between the MVD counts and the grade of the tumor. However, it is observed that well-differentiated OSCC showed the maximum MVD.

Bottom Line: Thirty-three cases of OSCC were stained with hematoxylin and eosin, (H and E) to confirm the diagnosis and immunohistochemically using VEGF and CD 34 antibody.The result was subjected to statistical analysis using analysis of variance (ANOVA) test and Fisher's exact test.Tumor MVD was found to be independent of expression of VEGF.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral and Maxillofacial Pathology, Bharati Vidyapeeth Dental college and Hospital, Pune, Maharashtra, India.

ABSTRACT

Background: Angiogenesis is the formation of new vessels from preexisting ones which takes place by capillary sprouting. It is seen in healing, at sites of injury and collateral circulation in ischemia. It is also seen in tumors, as once the original blood supply of a tumor is exhausted it cannot grow without further blood supply. Also as the angiogenic capacity of a tumor increases, its microvasculature, that is, microvessel density (MVD) also increases. Based on this literary evidence we carried out an immunohistochemical (IHC) study to observe the relationship between the expression of vascular endothelial growth factor (VEGF) [angiogenesis] and CD 34 [MVD] in oral squamous cell carcinoma (OSCC).

Aim: To evaluate the correlation between expression of VEGF and CD 34, the role of MVD in progression of OSCC and to compare the degree of angiogenesis in different grades of OSCC.

Settings and design: In this study we observed the relation between angiogenesis and MVD and the overall effect of this on oral cancer.

Materials and methods: Thirty-three cases of OSCC were stained with hematoxylin and eosin, (H and E) to confirm the diagnosis and immunohistochemically using VEGF and CD 34 antibody. The slides were evaluated for positivity and intensity of staining.

Statistical analysis: The result was subjected to statistical analysis using analysis of variance (ANOVA) test and Fisher's exact test.

Results: VEGF positivity as well as MVD was found to be independent of the grade of the tumor. Tumor MVD was found to be independent of expression of VEGF.

No MeSH data available.


Related in: MedlinePlus