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VEGF and CD 34: A correlation between tumor angiogenesis and microvessel density-an immunohistochemical study.

Kukreja I, Kapoor P, Deshmukh R, Kulkarni V - J Oral Maxillofac Pathol (2013)

Bottom Line: Thirty-three cases of OSCC were stained with hematoxylin and eosin, (H and E) to confirm the diagnosis and immunohistochemically using VEGF and CD 34 antibody.The result was subjected to statistical analysis using analysis of variance (ANOVA) test and Fisher's exact test.Tumor MVD was found to be independent of expression of VEGF.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral and Maxillofacial Pathology, Bharati Vidyapeeth Dental college and Hospital, Pune, Maharashtra, India.

ABSTRACT

Background: Angiogenesis is the formation of new vessels from preexisting ones which takes place by capillary sprouting. It is seen in healing, at sites of injury and collateral circulation in ischemia. It is also seen in tumors, as once the original blood supply of a tumor is exhausted it cannot grow without further blood supply. Also as the angiogenic capacity of a tumor increases, its microvasculature, that is, microvessel density (MVD) also increases. Based on this literary evidence we carried out an immunohistochemical (IHC) study to observe the relationship between the expression of vascular endothelial growth factor (VEGF) [angiogenesis] and CD 34 [MVD] in oral squamous cell carcinoma (OSCC).

Aim: To evaluate the correlation between expression of VEGF and CD 34, the role of MVD in progression of OSCC and to compare the degree of angiogenesis in different grades of OSCC.

Settings and design: In this study we observed the relation between angiogenesis and MVD and the overall effect of this on oral cancer.

Materials and methods: Thirty-three cases of OSCC were stained with hematoxylin and eosin, (H and E) to confirm the diagnosis and immunohistochemically using VEGF and CD 34 antibody. The slides were evaluated for positivity and intensity of staining.

Statistical analysis: The result was subjected to statistical analysis using analysis of variance (ANOVA) test and Fisher's exact test.

Results: VEGF positivity as well as MVD was found to be independent of the grade of the tumor. Tumor MVD was found to be independent of expression of VEGF.

No MeSH data available.


Related in: MedlinePlus

(a) Histopathological image shows poorly differentiated oral squamous cell carcinoma: VEGF staining intensity 3× (IHC stain, ×100). (b) Histopathological image shows poorly differentiated oral squamous cell carcinoma. (H&E stain, ×100)
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Figure 3: (a) Histopathological image shows poorly differentiated oral squamous cell carcinoma: VEGF staining intensity 3× (IHC stain, ×100). (b) Histopathological image shows poorly differentiated oral squamous cell carcinoma. (H&E stain, ×100)

Mentions: Using the above criteria, the following results were obtained. Table 1 and Figure 1 show the histopathological grades vs the intensity of staining with VEGF. Out of 15 cases of poorly differentiated squamous cell carcinoma, four cases showed negative staining with VEGF [Figure 2a and b], six cases showed a staining intensity of 1+ with VEGF, four cases showed a staining intensity of 2+ and one case showed a staining intensity of 3+ [Figure 3a and b]. In cases of moderately differentiated squamous cell carcinoma all six cases showed a staining intensity of 1+ [Figure 4a and b]. Out of 12 cases of well differentiated squamous cell carcinoma, eight cases showed a staining intensity of 1+ and four cases showed a staining intensity of 2+ with VEGF [Figure 5a and b]. By using Fisher's exact test P value was 0.094, since P > 0.05, therefore, there is no association between tumor grade and VEGF staining intensity.


VEGF and CD 34: A correlation between tumor angiogenesis and microvessel density-an immunohistochemical study.

Kukreja I, Kapoor P, Deshmukh R, Kulkarni V - J Oral Maxillofac Pathol (2013)

(a) Histopathological image shows poorly differentiated oral squamous cell carcinoma: VEGF staining intensity 3× (IHC stain, ×100). (b) Histopathological image shows poorly differentiated oral squamous cell carcinoma. (H&E stain, ×100)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3927337&req=5

Figure 3: (a) Histopathological image shows poorly differentiated oral squamous cell carcinoma: VEGF staining intensity 3× (IHC stain, ×100). (b) Histopathological image shows poorly differentiated oral squamous cell carcinoma. (H&E stain, ×100)
Mentions: Using the above criteria, the following results were obtained. Table 1 and Figure 1 show the histopathological grades vs the intensity of staining with VEGF. Out of 15 cases of poorly differentiated squamous cell carcinoma, four cases showed negative staining with VEGF [Figure 2a and b], six cases showed a staining intensity of 1+ with VEGF, four cases showed a staining intensity of 2+ and one case showed a staining intensity of 3+ [Figure 3a and b]. In cases of moderately differentiated squamous cell carcinoma all six cases showed a staining intensity of 1+ [Figure 4a and b]. Out of 12 cases of well differentiated squamous cell carcinoma, eight cases showed a staining intensity of 1+ and four cases showed a staining intensity of 2+ with VEGF [Figure 5a and b]. By using Fisher's exact test P value was 0.094, since P > 0.05, therefore, there is no association between tumor grade and VEGF staining intensity.

Bottom Line: Thirty-three cases of OSCC were stained with hematoxylin and eosin, (H and E) to confirm the diagnosis and immunohistochemically using VEGF and CD 34 antibody.The result was subjected to statistical analysis using analysis of variance (ANOVA) test and Fisher's exact test.Tumor MVD was found to be independent of expression of VEGF.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral and Maxillofacial Pathology, Bharati Vidyapeeth Dental college and Hospital, Pune, Maharashtra, India.

ABSTRACT

Background: Angiogenesis is the formation of new vessels from preexisting ones which takes place by capillary sprouting. It is seen in healing, at sites of injury and collateral circulation in ischemia. It is also seen in tumors, as once the original blood supply of a tumor is exhausted it cannot grow without further blood supply. Also as the angiogenic capacity of a tumor increases, its microvasculature, that is, microvessel density (MVD) also increases. Based on this literary evidence we carried out an immunohistochemical (IHC) study to observe the relationship between the expression of vascular endothelial growth factor (VEGF) [angiogenesis] and CD 34 [MVD] in oral squamous cell carcinoma (OSCC).

Aim: To evaluate the correlation between expression of VEGF and CD 34, the role of MVD in progression of OSCC and to compare the degree of angiogenesis in different grades of OSCC.

Settings and design: In this study we observed the relation between angiogenesis and MVD and the overall effect of this on oral cancer.

Materials and methods: Thirty-three cases of OSCC were stained with hematoxylin and eosin, (H and E) to confirm the diagnosis and immunohistochemically using VEGF and CD 34 antibody. The slides were evaluated for positivity and intensity of staining.

Statistical analysis: The result was subjected to statistical analysis using analysis of variance (ANOVA) test and Fisher's exact test.

Results: VEGF positivity as well as MVD was found to be independent of the grade of the tumor. Tumor MVD was found to be independent of expression of VEGF.

No MeSH data available.


Related in: MedlinePlus